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Öğe A novel affinity disks for bovine serum albumin purification(Humana Press, 2015) Tüzmen, Nalan; Kalburcu, Tülden; Uygun, Deniz Aktaş; Akgöl, Sinan; Denizli, AdilThe adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as -58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process.Öğe ?-Amylase immobilization onto dye attached magnetic beads: Optimization and characterization(Elsevier Science Bv, 2012) Tüzmen, Nalan; Kalburcu, Tülden; Denizli, AdilMagnetic poly(2-hydroxyethylmethacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of Fe3O4 nano-powder. Cibacron Blue F3GA (CB) was covalently immobilized to the mPHEMA beads via nucleophilic substitution reaction between chloride of its triazine ring and hydroxyl groups of HEMA under alkaline conditions. The mPHEMA/CB beads (100-140 mu m in diameter) carrying 68.3 mu mol CB/g polymer were used in a-amylase adsorption studies to assess the effects of pH, initial protein concentration, temperature and ionic strength on enzyme activity. Maximum adsorption capacity of mPHEMA/CB beads was found to be 401 +/- 11 mg/g carrier. The adsorbed amounts of a-amylase per unit mass of magnetic beads reached a plateau value at about 1.0 mg/mL at pH 5.0. The optimal pH for free and immobilized a-amylase was 7.0 and 8.0, respectively. The immobilized enzyme exhibited better thermostability than the free one. The free enzyme lost all of its activity within 35 days whereas the immobilized enzyme lost about 27% of its activity during the same period. It was also observed that the enzyme could be repeatedly adsorbed and desorbed onto the mPHEMA/CB beads. (C) 2012 Elsevier B.V. All rights reserved.Öğe Bacterial cellulose nanofibers for separation, drug delivery, wound dressing, and tissue engineering applications(Elsevier, 2022) Tamahkar, Emel; Topçu, Aykut Arif; Perçin, Işık; Aslıyüce, Sevgi; Denizli, AdilBacterial cellulose (BC) nanofibers with high purity, high surface/volume ratio, high biocompatibility, high porosity, high tensile strength, and high water holding capacity are emerging attractive biomaterial. BC nanofibers can be prepared simply via bacterial incubation withalow cost inadetermined shape owing to its in-situ moldability. BC nanofibers due to its structural and morphological properties have demonstrated great potential asanative form or with various modifications using polymers, micro/nanoparticles, and small molecules for diverse application areas. This review aims to discuss the applicability and efficiency of the BC nanofibers and its composites focusing on the unique characteristics of BC in the fields of separation, drug delivery, wound dressing, and tissue engineering. It overviews the recent developments about the fabrication methods of BC that was used asaseparation medium, adrug delivery vehicle, awound dressing material, andascaffold.Öğe Cadmium removal performances of different dye ligands attached cryogel disks(Croatian Chemical Society, 2015) Keleş, Burak; İnanan, Tülden; Tüzmen, Nalan; Denizli, AdilPoly(HEMA) cryogel disks were synthesized by free radical polymerization of 2-hydroxyethylmethacrylate (HEMA), and then Cibacron Blue F3GA (CB), Reactive Green 19 (RG) and Congo Red (CR) were immobilized as dye ligands. Disks were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and swelling degree, porosity calculations. Cd2+ adsorption experiments were performed for 60 min. Maximum adsorption capacities were determined as 25.5 mg/g; 48.0 mg/g and 28.5 mg/g at pH = 7.0 for poly(HEMA)-CB; poly(HEMA)-RG and poly(HEMA)-CR, respectively. Langmuir isotherm fitted best with the adsorption data and adsorption thermodynamics showed that Cd2+ adsorption is thermodynamically favorable and a physisorption process. A great majority of adsorbed Cd2+ desorbed with 1 M NaCl and cryogel disks can be re-used in adsorption experiments. Cd2+ removal efficiencies of disks from human plasma are approximately 45 %. Dye-attached cryogel disks synthesized in this study have potential in use for environmental and therapeutic applications.Öğe Cholesterol removal onto the different hydrophobic nanospheres: A comparison study(Elsevier, 2014) Kalburcu, Tülden; Öztürk, Nevra; Tüzmen, Nalan; Akgöl, Sinan; Denizli, AdilThe aim of this study is to prepare two different hydrophobic polymeric nanospheres for cholesterol removal. Poly(HEMA-MAT) nanospheres with an average size of 100 nm and poly(HEMA-MAP) nanospheres with an average size of 158 nm were produced by surfactant free emulsion polymerization of HEMA and MAT and MAP monomers. These hydrophobic nanospheres were characterized by FTIR, SEM, elemental analysis, particle size and surface area measurements. Cholesterol removal experiments were performed in a batch experimental set-up and removal medium was methanol. Cholesterol adsorption capacity of poly(HEMA-MAP) nanospheres was approximately three times higher than that of poly(HEMA-MAT) nanospheres. (C) 2013 The Korean Society of Industrial and Engineering Chemistry.Öğe Conducting polymers for gene delivery(CRC Press, 2022) Topçu, Aykut Arif; Özgür, Erdoğan; Denizli, AdilGene therapy is an alternative approach to insert the gene into a patient's cell to treat various diseases such as cancer, inherited disorders, and viral infections instead of using drugs or operations. For this aim, the viral and nonviral vectors are used in gene delivery studies and the nonviral vectors received significant attention because of simplicity, low-immune response, and controllability. Conducting polymers (CPs) known as Nobel Prize winner materials with unique features such as ease of synthesis, in vivo and in vitro biocompatibility, electric conductivity are promising materials for wide applications, e.g., biosensor platforms, photocatalytic applications, and biomedical studies. Furthermore, depending on the redox state, the molecules could be entrapped or released by applying the electrical stimulation; so, these conjugated polymers are also potentially used in gene delivery studies. In this context, the widely used CPs, their chemical synthesis, their interactions with DNA, siRNA, their entry into the cells, and their gene delivery performances are highlighted.Öğe Design and preparation of imprinted surface plasmon resonance (SPR) nanosensor for detection of Zn(II) ions(Taylor & Francis Online, 2019) Jalilzadeh, Mitra; Çimen, Duygu; Özgür, Erdoğan; Esen, Cem; Denizli, AdilA novel Zn(II) ions imprinted poly (2-hydroxyethyl Methacrylate-N-methacryloyl-(L)-histidine methyl ester) poly(HEMAH) surface plasmon resonance (SPR) nanosensor were designed for detection of Zn(II) ions in aqueous solution and artificial plasma providing a low cost, rapid and reliable results compared to other techniques such as atomic absorption spectroscopy, inductively coupled plasma-mass spectrometer, X-ray fluorescence with synchrotron radiation. Zn(II) ions imprinted nanofilm on the SPR chip surface was synthesized by bulk polymerization. Characterization of Zn(II) ions imprinted nanosensor was performed by contact angle measurement, atomic force microscopy (AFM), ellipsometry and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR). Designed nanosensor was applied for selective detection of Zn(II) ions in aqueous solution within the range of 0.5–1.0 mg/mL. The limit of detection (LOD) and limit of quantification (LOQ) were calculated as 0.19 and 0.64 ng/mL, respectively. Association kinetics analysis, Scatchard, Langmuir, Freundlich, Langmuir–Freundlich, Tempkin and Dubinin-Radushkevich isotherms were analyzed to the experimental data in order to identify the adsorption behavior. The selectivity of the SPR nanosensor was examined by using competitive metal ions such as Cd(II), Cu(II), Pb(II), and Fe(II). To evaluate the imprinting effect of Zn(II) ions imprinted (MIP) and non-imprinted (NIP) nanosensor was also prepared as the control. Repeatability of the response signal was tested by four times adsorption–desorption–regeneration cycle.Öğe Immobilization of catalase via adsorption onto metal-chelated affinity cryogels(Elsevier, 2012) Tüzmen, Nalan; Kalburcu, Tülden; Denizli, AdilA poly (acrylamide-allylglycidyl ether) [p(AAm-AGE)] cryogel was prepared by radical polymerization of acrylamide (AAm) and allylglycidyl ether (AGE). Cibacron Blue F3GA (CB) was covalently attached to the p(AAm-AGE) cryogel via the reaction between the chloride groups of the reactive dyes and the epoxide groups of the AGE. The CB-attached p(AAm-AGE) cryogel was chelated with Fe3+ ions. This immobilized metal ion affinity chromatography (IMAC) cryogel carrying 25.8 +/- 2.0 mu mol Fe3+ ions was used in adsorption studies to interrogate the effects of pH, protein initial concentration, flow rate, temperature and ionic strength on enzyme activity. Maximum adsorption capacities were found to be 75.7 +/- 1.2 mg/g for p(AAm-AGE)-CB-Fe3+ cryogels and 60.6 +/- 1.0 mg/g for p(AAm-AGE)-CB cryogels, respectively. The adsorbed amounts of catalase per unit mass of cryogel reached a plateau value at about 1.5 mg/mL at pH 6.0. The K-m values were found to be 0.73 +/- 0.02 g/L for the free catalase and 0.18 +/- 0.02 g/L for the immobilized catalase. The V-max value of free catalase (2.0 x 10(3) U/mg enzyme) was found to be lower than that of the immobilized catalase (2.5 x 10(3) U/mg enzyme). It was also observed that the enzyme could be repeatedly adsorbed and desorbed onto the p(AAm-AGE)-CB-Fe3+ cryogel. (C) 2011 Elsevier Ltd. All rights reserved.Öğe Immobilized metal ion affinity nanospheres for ?-amylase immobilization(Scientific Technical Research Council Turkey-Tubitak, 2014) Kalburcu, Tülden; Tüzmen, Münire Nalan; Akgöl, Sinan; Denizli, AdilImmobilized metal chelate affinity chromatography (IMAC) support was practiced for alpha-amylase immobilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni2+ [p(HEMA-MAT)-Ni2+] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMA-MAT)-Ni2+ nanospheres were carried out by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). In addition, average particle size, size distribution, and surface charge were specified. The amount of N-methacryloylamidotryptophan (MAT) incorporated to polymer was determined as 1.95 mmol/g polymers by using nitrogen stoichiometry. The specific surface areas of poly(hydroxyethylmethacrylate) [p(HEMA)] and p(HEMA-MAT) nanospheres were calculated as 1856 m(2)/g and 1914 m(2)/g, respectively. Protein adsorption increased with increasing initial protein concentration and maximum alpha-amylase adsorption on p(HEMA-MAT)-Ni2+ nanospheres was observed at pH 4.0. Both free and immobilized alpha-amylase showed pH optimum at pH 7.0. It was determined that the immobilized alpha-amylase had better thermostability than the free one. Immobilization of the enzyme did not significantly change the kinetic parameters. The storage stability of alpha-amylase increased upon immobilization. It was also observed that p(HEMA-MAT)-Ni2+ nanospheres can be repeatedly used for alpha-amylase immobilization.Öğe Indian saffron - Turmeric (Curcuma longa) embedded supermacroporous cryogel discs for heavy metal removal(Biointerface Research Applied Chemistry, 2019) Aslıyüce, Sevgi; Bereli, Nilay; Topçu, Aykut; Ramteke, Pramod W.; Denizli, AdilCryogels are used in a variety of environmental and biotechnological processes. Cryogels are polymeric materials with large pores and open flow channels. Turmeric is a very popular spice, especially in India, which has been shown to contain curcumin alkaloids to treat a variety of many diseases. Playing a protective and therapeutic role against the diseases results from being able to bind to various targets. In this study, Indian saffron (Turmeric) embedded poly(2-hydroxyethyl methacrylate) cryogel discs (Tur-PHEMA/CDs) have been prepared to remove heavy metal ions from waste-water, which is a major environmental problem by utilizing the heavy metal binding property of turmeric. Tur-PHEMA/CDs were used to remove Cu(II), Pb(II), Cd(II) ions. Poly(2-hydroxyethyl methacrylate) cryogel discs (PHEMA/CDs) were also used as control polymer. The prepared cryogels are characterized by multiple experimental tests. The Tur-PHEMA/CDs and PHEMA/CDs with respectively swelling ratio of 83.6% and 71.2% were used in heavy metal ions adsorption studies. pH values of the solution were changed in the range of 3.0-6.0 to determine optimum pH. Maximum adsorption capacities of the Tur-PHEMA/CDs from aqueous solution were 18.36 mg/g for Cu(II), 8.99 mg/g for Pb(II) and 5.76 mg/g for Cd(II). The affinity order of heavy metal ions on mass basis was Cu(II) > Pb(II) > Cd(II) from synthetic wastewater. EDTA solution (0.5 M) was used for desorbing of heavy metal ions.Öğe Inspirations of biomimetic affinity ligands: a review(American Chemical Society, 2022) Topçu, Aykut Arif; Kılıç, Seçkin; Özgür, Erdo?an; Türkmen, Deniz; Denizli, AdilAffinity chromatography is a well-known method dependent on molecular recognition and is used to purify biomolecules by mimicking the specific interactions between the biomolecules and their substrates. Enzyme substrates, cofactors, antigens, and inhibitors are generally utilized as bioligands in affinity chromatography. However, their cost, instability, and leakage problems are the main drawbacks of these bioligands. Biomimetic affinity ligands can recognize their target molecules with high selectivity. Their cost-effectiveness and chemical and biological stabilities make these antibody analogs favorable candidates for affinity chromatography applications. Biomimetics applies to nature and aims to develop nanodevices, processes, and nanomaterials. Today, biomimetics provides a design approach to the biomimetic affinity ligands with the aid of computational methods, rational design, and other approaches to meet the requirements of the bioligands and improve the downstream process. This review highlighted the recent trends in designing biomimetic affinity ligands and summarized their binding interactions with the target molecules with computational approaches.Öğe Microcontact imprinted plasmonic nanosensors: Powerful tools in the detection of salmonella paratyphi(MDPI, 2017) Perçin, Işık; İdil, Neslihan; Bakhshpour, Monireh; Yılmaz, Erkut; Mattiasson, Bo; Denizli, AdilIdentification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 x 10(6)-15 x 10(6) CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 x 10(6) CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.Öğe Molecularly imprinted cryogel membranes for mitomycin C delivery(TAYLOR & FRANCIS, 2017) Öncel, Pınar; Çetin, Kemal; Topçu, Aykut Arif; Yavuz, Handan; Denizli, AdilIn this study, cryogel-based implantable molecularly imprinted drug delivery systems were designed for the delivery of antineoplastic agent. Mitomycin C imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-glutamic acid) cryogel membranes were produced by free-radical bulk polymerization under partially frozen conditions. The membranes were characterized by swelling tests, Fourier transform infrared spectroscopy, scanning electron microscopy, surface area measurements and in vitro hemocompatibility tests. In vitro delivery studies were carried out to examine the effects of cross-linker ratio and template content. Mitomycin C imprinted cryogel membranes have megaporous structure (10-100 mu m in diameter). The cumulative release of mitomycin C was decreased with increasing cross-linking agent ratio and increased with the amount of template in the cryogel structure. The nature of transport mechanism of the mitomycin C from the membranes was non-Fickian.Öğe Molecularly imprinted polymers as a tool for biomolecule separation(Elsevier, 2017) Andaç, Müge; Baydemir, Gözde; Denizli, AdilThe publications in molecularly imprinted materials have increased drastically in recent years, with the development of polymerization systems. The memory in shape, interaction and size of binding cavities has allowed the use of these materials within different applications, particularly in affinity purification and separation in chromatography, sample preparation, and detection in biosensors. Due to their high selectivity, specificity, efficient mass transfer and good reproducibility, molecularly imprinted materials have become attractive for researchers in biomolecular and biomacromolecular recognition. In this review, the recent developments of molecularly imprinted materials in affinity separation and purification systems are reviewed comprehensively. Some significant applications were also highlighted for further investigation.Öğe MXenes and Its Composites for Biomedical Applications(Wiley, 2024) Topçu, Aykut Arif; Kılıç, Seçkin; Denizli, AdilMXenes, referred to as 2D emerging materials from the transition metal carbides, nitrides, or carbonitrides family, have taken the attention of the scientific community since 2011 because of having unique features, for instance, biocompatibility, high hydrophilicity, mechanical, electrical, optical, and antibacterial properties, as well as photothermal conversion efficiency (PCE), large surface area, and so on. Due to the combination of their versatile chemistry and tunable properties, MXenes play a crucial role in various fields e.g. electrocatalysis, energy storage, corrosion protection, electromagnetic, optical, electronic, and high-temperature structural applications. Furthermore, ease of the surface modification of these emerging materials with the biomolecules, nanoparticles, and quantum dots can enhance the superior features of MXenes and make them favorable materials used in various biomedical applications and bioimaging. The new generation of 2D materials has paved way the for biosensor studies, including wearable biosensing platforms, point-of-care devices, theranostics, photothermal therapy of cancer, drug delivery studies, and antibacterial applications. In this context, we first introduce the basic properties, unique features, and preparation protocols of MXene and its composites. After that, we highlighted the possible use of MXenes and their composites for different biomedical applications with recent and relevant research articles.Öğe Nanobiosensors for mycotoxins detection in foodstuff: Qualitative and quantitative assessments(Elsevier, 2024) Çalışır, Merve; Özgür, Erdoğan; Çimen, Duygu; Topçu, Aykut Arif; Erkek, Muhammed; Bereli, Nilay; Denizli, AdilMycotoxins are naturally toxic compounds with low molecular weight and formed as a result of the secondary metabolism of fungi (mold) species. Since they are harmful to many living organisms, cost-effective, sensitive, and reliable detection methods are crucial for preventing the unwanted side effects of these toxic metabolites. Enzyme-linked immunoassay (ELISA), chromatographic methods like thin layer chromatography (TLC), and biosensors are often used as analytical approaches for the detection of mycotoxins. In this context, we summarized the traditional methods for mycotoxin detection and focused on the potential use of nanobiosensor platforms for mycotoxin sensing in foodstuffs.Öğe Plastic antibody based surface plasmon resonance nanosensors for selective atrazine detection(Elsevier, 2017) Yılmaz, Erkut; Özgür, Erdoğan; Bereli, Nilay; Türkmen, Deniz; Denizli, AdilThis study reports a surface plasmon resonance (SPR) based affinity sensor system with the use of molecular imprinted nanoparticles (plastic antibodies) to enhance the pesticide detection. Molecular imprinting based affinity sensor is prepared by the attachment of atrazine (chosen as model pesticide) imprinted nanoparticles onto the gold surface of SPR chip. Recognition element of the affinity sensor is polymerizable form of aspartic acid. The imprinted nanoparticles were characterized via FTIR and zeta-sizer measurements. SPR sensors are characterized with atomic force microscopy (AFM), scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The imprinted nanoparticles showed more sensitivity to atrazine than the non-imprinted ones. Different concentrations of atrazine solutions are applied to SPR system to determine the adsorption kinetics. Langmuir adsorption model is found as the most suitable model for this affinity nanosensor system. In order to show the selectivity of the atrazine-imprinted nanoparticles, competitive adsorption of atrazine, simazine and amitrole is investigated. The results showed that the imprinted nanosensor has high selectivity and sensitivity for atrazine. (C) 2016 Elsevier B.V. All rights reserved.Öğe Poly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M(MDPI Multidisciplinary Digital Publishing Institute, 2020) Bakhshpour, Monireh; Topçu, Aykut Arif; Bereli, Nilay; Alkan, Hüseyin; Denizli, AdilHuman immunoglobulin M (hIgM) antibodies are considered as hopeful tools for diseases therapy. Therefore, chromatography approaches are used to purify hIgM with a single step. In this study, we prepared a poly(hydroxyethyl methacrylate) based immunoaffinity p(HEMA-I) cryogel column by using cyanamide to immobilize antihuman immunoglobulin on the p(HEMA) cryogel for purification of hIgM in aqueous solution and artificial human plasma. The characterization of the p(HEMA) cryogel column was performed by using a scanning electron microscope (SEM), micro-computerized tomography (µ-CT), Fourier transform infrared spectroscopy (FTIR), swelling degree and macro-porosity. Further, the optimizations of various parameters were performed such as, pH, ionic strength, temperature and concentration of hIgM in aqueous solutions. In addition, the Langmuir adsorption model was supported by experimental results. Maximum adsorbed amount of hIgM corresponded to 11.1 mg/g at pH 5.75 [morpholino ethanesulfonic acid (MES buffer)]. Our results indicated that the p(HEMA-I) cryogel column can be reused at least 10 times without significant loss in adsorption capacity. As a natural source, artificial human plasma was selected for hIgM adsorption and the purity of hIgM was evaluated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). © 2020 by the authors. Licensee MDPI, Basel, Switzerland.Öğe Preparation of cryogel columns for depletion of hemoglobin from human blood(Taylor & Francis, 2016) Derazshamshir, Ali; Baydemir, Gözde; Yılmaz, Fatma; Bereli, Nilay; Denizli, AdilIn this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.Öğe Preparation of molecular imprinted injectable polymeric micro cryogels for control release of mitomycin C(Springer Science and Business Media Deutschland GmbH, 2023) Zabihi, Soheil; Bakhshpour, Monireh; Çalışır, Merve; Topçu, Aykut Arif; Denizli, AdilIn this work, microscale poly-2-hydroxyethyl methacrylate [p(HEMA)]-based cryogels were fabricated as a drug delivery material using molecular imprinting technology for controlled release of mitomycin C (MMC) as an anti-cancer drug. MMC imprinted pHEMA-based micro cryogels (pMIPs) were prepared according to free-radical polymerization by using N-methacryloyl-(l)-histidine methyl ester (MAH) as an amino-acid-based polymerizable functional monomer using a micro stencil array chip with microwells of 200 ?m diameter and 500 ?m thickness. Following that, scanning electron microscope, swelling test, and Fourier Transform Infrared Spectroscopy were used for the characterization studies of pMIPs. After the characterization studies, MMC release performance of pMIPs was investigated towards the different pH values and various MMC concentrations in the aqueous solutions. The in vitro cytotoxicity studies of the pMIPs and the non-imprinted pHEMA based micro cryogels (pNIPs) were examined using L929 cell line. According to the experimental findings, the incorporation of MAH monomer could increase the release performance of pMIPs and the release of MMC from the pMIPs was non-Fickian in the aqueous solution. pMIPs did not show any noticeable cytotoxicity and could be potentially used as a new drug carrier for MMC release.
