Yazar "Boydak, Didem" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Black cumin may be a potential drug for development of carbontetrachloride-induced lung damage in rats
    (Mattioli 1885 S.p.A., 2016) Aslan, Abdullah; Boydak, Didem; Can, Muhammed İsmail; Kuloğlu, Tuncay; Başpınar, Serpil
    The study examines whether Black cumin (Nigella sativa L.) plays a protective role against the damage in the lung by administering carbontetrachloride (CCl4) to rats. 28 male Wistar albino (n=28, 8 weeks old) rats were used in the study. The rats divided into 4 groups according to their live weights. The groups were: (i) Negative Control (NC): Normal water consuming group to which no CCl4 and Black cumin (BC) is administered; (ii) Positive Control (PC): Normal water consuming group to which no CCl4 is administered but BC is administered; (iii) CCl4 Group: Normal water consuming and group to which CCl4 is administered (1.5 ml/kg live weight, ip); (iv) CCl4 + BC group: CCl4 and BC administered group (1.5 ml/kg live weight, ip). Tissue apoptotic index was examined via TUNEL method. MDA (malondialdehyde) determination in lung tissue was made using spectrophotometer. As a results, MDA amount decreased in the CCl4 + BC group (6,33 ± 1,54 nmol/g) in comparison to CCl4 group (8,66 ± 1,58 nmol/g) whereas it was observed in the CCl4+BC group (15,35 ± 0,21%) that the apoptotic index (TUNEL results) decreases in comparison with the CCl4 group (27,48 ± 0,28%) thus approaching normal values. DNA damage ratio decreased in the CCl4 + BC group in comparison to CCl4 group. These results show that BC plant protects the lung against oxidative damage. © Mattioli 1885.
  • Küçük Resim
    Öğe
    Nigella sativa improves the carbon tetrachloride-induced lung damage in rats through repression of erk/akt pathway
    (Bangladesh Pharmacological Society, 2015) Aslan, Abdullah; Boydak, Didem; Can, Muhammed İsmail; Kuloğlu, Tuncay
    The objective of this study was to examine whether Nigella sativa plays a protective role against the damage in the lung by administering carbon tetra-chloride (CCl4) to rats. Male Wistar albino (n=28, 8 weeks old) rats were divided into 4 groups: a) negative control: Normal water consuming group to which no CCl4 and N. sativa was administered; b) Positive control: Normal water consuming group to which no CCl4 was administered but N. sativa was administered; c) CCl4 Group: Normal water consuming and group to which CCl4 was administered (1.5 mL/kg, ip); d) N. sativa plus CCl4group: CCl4and N. sativa administered group (1.5 mL/kg, ip). Caspase-3, caspase -9, erk, akt protein syntheses were examined via Western blotting. Malondialdehyde determination in lung tissue was made using spectrophotometer. As a results, malondialdehyde amount was decreased in the CCl4 plus N. sativa group in comparison to CCl4 group whereas caspase-3, caspase-9 was increased and erk, akt had decreased. These results show that N. sativa protects the lung against oxidative damage.