Yazar "Ataman, Mehmet Bozkurt" seçeneğine göre listele

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    Combination of cysteamine and lipoic acid ımproves the post-thawed bull sperm parameters
    (2016) Güngör, Şükrü; Aksoy, Adil; Yeni, Deniz; Avdatek, Fatih; Öztürk, Caner; Ataman, Mehmet Bozkurt; Coyan, Kenan; Bucak, Mustafa Numan
    The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P>0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P < 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull sperm
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    DNA damaging effect of paclitaxel in the epididymal sperms as a chemotherapeutic agent and possible remedies to prevent this effect: A study on reproductive potential of male cancer patients of reproductive age
    (Elsevier Ltd., 2019) İli, Pınar; Sarı, Fikret; Bucak, Mustafa Numan; Öztürk, Caner; Güngör, Şükrü; Ataman, Mehmet Bozkurt
    Cancer is a major public health problem, young cancer patients therefore undergo chemotherapy, andmost of them may lose their fertility. DNA damage level provides important clues about the quality andreproductive potential of spermatozoa. In this study, we evaluated the levels of both DNA fragmentationand abnormal DNA integrity in the epididymal sperms of New Zealand rabbit (Oryctolagus cuniculus)after cryopreservation using the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay and the toluidine blue (TB) staining methods and assessed the effects of pacli-taxel, resveratrol,L-glutamine (LG), and basal medium eagle (BME) solution on DNA damage. Paclitaxelinduced the levels of both DNA damages in the sperms, but resveratrol ameliorated this effect. LG andBME supplementation to the extender prevented the sperm samples from DNA fragmentation aftercryopreservation. Chemotherapy drugs containing paclitaxel can cause the sperm DNA to be damaged,and hence adversely affect the fertility of male cancer patients of reproductive age. The administration ofresveratrol together with paclitaxel may ameliorate the DNA damage inducing effect of paclitaxel. Spermbanking and cryopreservation with the appropriate cryoprotectants such as LG and BME prior to cancertreatment can also be suggested to all male cancer patients of reproductive age facing cancer treatmentfor fertility preservation.
  • Küçük Resim
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    Effect of paclitaxel and resveratrol on New Zealand rabbit semen
    (Wolters Kluwer Medknow Publications, 2018) Öztürk, Caner; Ataman, Mehmet Bozkurt
    Objective: To examine the effects of paclitaxel and resveratrol on rabbit semen. Methods: This study consisted of four groups: control group (40 mL saline), paclitaxel group (5 mg/kg paclitaxel), resveratrol group (4 mg/kg resveratrol) and paclitaxel+resveratrol group (5 mg/kg paclitaxel+4 mg/kg resveratrol). Administrations were i.v. (in 40 mL saline) and continued 8 weeks. Sperm motility was evaluated using phase-contrast microscopy. Mitochondrial activity, membrane and acrosome integrity were performed by fluorescence staining. Lipid peroxidation, total glutathione and antioxidant potential levels were determined by spectrophotometry. Results: Paclitaxel decreased the sperm motility and fluorescence staining results compared to the control (P<0.05). The paclitaxel and resveratrol group showed better results of the same parameters compared to the paclitaxel group (P<0.05). No significant difference was observed in lipid peroxidation, total glutathione, antioxidant potential and fertility results (P>0.05). Results of this study showed that paclitaxel decreased semen parameters and resveratrol had a protective effect on these parameters. Conclusions: Paclitaxel has negative effects on spermatological indicators and biochemical assays, while resveratrol prevents these negative effects of paclitaxel.
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    Effects of arginine and trehalose on post-thawed bovine sperm quality
    (Akademiai Kiado Rt., 2017) Öztürk, Caner; Güngör, Şükrü; Ataman, Mehmet Bozkurt; Bucak, Mustafa Numan; Başpınar, Nuri; İli, Pınar; İnanc, Muhammed Enes
    The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 degrees C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 +/- 8.21%), CASA motility (12.2 +/- 5.69%) and progressive motility (3.52 +/- 2.13%), compared with the controls (43 +/- 2.73%, 55.4 +/- 6.78% and 33.48 +/- 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 +/- 3.99% and 44.1 +/- 2.18%) compared with the control (13 +/- 8.15 and 31.7 +/- 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.