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A novel polymerase chain reaction to detect brucella canis in dogs
(Kafkas Üniversitesi, 2015) Aras, Zeki; Taşpınar, Mehmet; Aydın, İbrahim
In this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis in vaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and the deletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were used for the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated by only amplification of 214 bp product. It detected at least 2.7 x 101 CFU/g of bacteria diluted in vaginal swab samples indicates that the polymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides a simple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.
Antibiogram Results of Escherichia coli in Calf Diarrhea and Escherichia coli Bacteria in Aksaray Province in The Last Three Months
(Afyon Kocatepe Üniv. Veteriner Fak., 2023) Haydardedeoglu, Ali Evren; Aydemir, Melek; Şenoğlu, Elif Selin; Aras, Zeki
Escherichia coli is a gram-negative, facultative anaerobic, motile, non-spore-forming rod-shaped bacterium belonging to the Enterobacteriaceae family. Pathogenic E. coli are divided into two groups: extraintestinal and intestinal. Intestinal Escherichia coli pathotypes: enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), Vero- or Shiga-toxin-producing E. coli (VTEC or STEC), enterohemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). Extraintestinal pathogenic E. coli: These can be listed as septicemic pathogenic E. coli, uropathogenic E. coli, avian pathogenic E. coli, breast pathogenic E. coli and those that cause uterine infections, endometrial pathogenic E. coli. Enterotoxigenic Escherichia coli (ETEC) is the most common cause of neonatal diarrhea in calves and is a bacterial disease that colonizes the small intestine, produces enterotoxin, and occurs among calves during the neonatal period. The aim of this study is to determine the E.coli isolates that cause neonatal calf diarrhea in the Aksaray region between January, February and March 2021. To be used in the study, internal organ samples (liver, heart, lung and mesenteric lymph node) of neonatal calves that died due to diarrhea were taken from 20 different cattle farms in the Aksaray region. One calf that died in each farm was included in the sampling, and a total of 20 calves were sampled. E.coli was isolated in 12 (60%) of the internal organ samples covering these three months. According to the antibiogram results of the samples, 50% of the isolates were converted to Amoxicillin and Erythromycin, 33.3% to Tetracycline, 58.3% to Trimethoprim-sulfamethoxazole, 66.6% to Streptomycin, 75% to Flofenicol, Gentamicin and Enrofloxacin, % 83.3 of them were found to be sensitive to Cefloxacin and Cefloxacinium.
Brucella dna'sının teshisi amacıyla altın nanoparçacıklarının kullanıldıgı aunps-prob nanoteshis metodunun gelistirilmesi
(TÜBİTAK, 2017) Aras, Zeki; Uçan, Uçkun Salih; Sayın, Zafer
Brusellozis; hayvanlarda genital organlara yerleşerek yavru atmalara ve infertiliteye neden olmakta, ülkelerin ekonomilerini olumsuz yönde etkilemektedir. Son yıllarda, bakteriyel hastalıkların teşhisi amacıyla altın nano parçacıklarının (AuNPs) kullanıldığı AuNPs-prob nanoteşhis yöntemleri geliştirilmiştir. Bu metot ile en az miktardaki genomik DNA’lar bile kullanılan nano parçacıklardan dolayı başarı ile tespit edilmektedir. Fakat Brucella enfeksiyonlarının hızlı nano teşhisi amacıyla geliştirilmiş herhangi bir AuNPs-prob nanoteşhis metodu bulunmamaktadır. Bu araştırma projesi ile, ülkemiz hayvancılığına ciddi zararlar veren brusellozisin direk teşhisi amacıyla hızlı, güvenilir, yüksek hassasiyete sahip ve DNA amplifikasyonu gerektirmeyen AuNPs-prob nanoteşhis yönteminin geliştirilmesi amaçlandı. Bu amaçla, Brucella cinsine özgü 16S-23S rRNA veya omp2 gen bölgeleri ile hibridize olabilecek 3 prob dizayn edildi, elde edilen problar altın nanoparçacıklarına tutturularak AuNPs-prob nanoteşhis metodu geliştirildi. Metodun spesifite, sensitivite ve tekraralanabilirliğini test etmek için 50 adet koyun aborte fetüs mide içeriği, 50 adet inek aborte fetüs mide içeriği, 50 adet inek süt örneği ve 50 adet koyun süt örneği kullanıldı. Örnekler Brucella varlığı yönünden kültür ve PZR yöntemleri ile incelendi. Brucella yönünden pozitif bulunmuş atık fetüs örneklerinin ait olduğu 12 sürüden toplanmış 410 kan örneği RBPT ve SAT testleri ile incelendi ve sonuçları AuNPs-prob nanoteşhis yöntem sonuçları ile karşılaştırıldı. Toplam 200 saha örneğinin 28’i kültür, 30’u PZR ve 25’i AuNPs-prob nanoteşhis yöntemi ile Brucella yönünden pozitif olarak bulundu. Geliştirilen yöntemin belirleyebildiği en az DNA miktarı B. melitensis, B. aborus ve B. canis referans suşları için sırasıyla 93, 101 ve 88 ng olarak tespit edildi. AuNPs-prob nanoteşhis metodunun sensitivitesi %89.3, spesifitesi %100 ve tekrarlanabilirliği %100 olarak hesap edildi. Serolojik olarak Brucella pozitif olan 12 sürüden toplanmış 12 aborte fetüs mide içeriğinin 11’inde Brucella DNA’sı AuNPs-prob nanoteşhis yöntemi ile tespit edildi. Sonuç olarak, brusellozisin teşhisine yönelik AuNPs-prob nanoteşhis yöntemi bu proje kapsamında geliştirildi. Bu yöntem ile brusellozisin hem insanlarda hem de hayvanlarda hızlı, ucuz, güvenli ve en önemlisi küçük laboratuarlarda pahalı PZR vb cihazlara gereksinim duyulmadan teşhisi sağlanacaktır.
Decontamination effect of zinc oxide nanoparticles, rosmarinic acid and anatolian propolis on foodborne bacteria
(Turkish Science and Technology Publishing (TURSTEP), 2022) Kevenk, Tahsin Onur; Aras, Zeki
Zinc oxide (ZnO) has been used in the pharmaceutical, cosmetic, paint, textile, and food industries to coat surfaces and absorb UV rays. As a result of its antimicrobial properties in nanoscale, it may be a promising chemical for decontamination. Zinc oxide nanoparticles (ZnO-NPs) are generally considered safe (GRAS) for their stability under challenging processing conditions. Our study investigated the antimicrobial activities of rosmarinic acid and propolis, prepared at different concentrations together with ZnO-NPs. For this purpose, six leading foodborne pathogens and a starter culture were chosen. The invitro decontamination effects of ZnO-NPs, rosmarinic acid, and propolis combinations on selected bacteria in the first 24 hours were demonstrated by bacterial counts at regular intervals. According to our results, propolis and ZnO-NPs showed remarkable results together. In addition, rosmarinic acid’s lower concentrations were also found to induce the decontamination effect of ZnO-NPs. Although the antimicrobial effect of ZnO-NPs, when used alone, was less than when used in combination, it was still found to be sufficient.
Detection and molecular typing of Clostridium perfringens isolates from beef, chicken and turkey meats
(Elsevıer Scı Ltd, 2015) Aras, Zeki; Hadımlı, Hasan Hüseyin
Here we describe a study investigating the presence of Clostridium perfringens strains in meat samples and the toxin genes in the isolates by PCR. This study, for the first time, demonstrated the presence of C perfringens type E in turkey meats, while C perfringens type C strains isolated from chicken meats. (C) 2014 Elsevier Ltd. All rights reserved.
Determination of Carbapenem-Resistant Enterobacteriaceae from Chicken Meat by Advanced Modified ISO 21528-1:2017 Method
(University of Agricultural Sciences and Veterinary, 2022) Kevenk, Tahsin Onur; Aras, Zeki
Antibiotic resistance is critical today, and carbapenem-resistant Enterobacteriaceae (CRE) are the current reflection of this threat in terms of public health. Our study aimed to investigate the effectiveness of a known ISO method used to detect of CREs in chicken meat sold in the Aksaray Region. A total of 150 chicken samples (50 drumsticks, 50 breasts, and 50 wings) were analyzed in terms of CRE by modifying the ISO 21528-1:2017 method. For this purpose, meropenem and ertapenem powders were added into buffered peptone water and Violet Red Bile Glucose Agar at the resistance levels determined in EUCAST. At the same time, target DNA extraction was performed from all samples with a tissue isolation kit (Hybrigen) and stored for PCR to support and strengthen our results to compare the cultural method's results. According to the results of the cultural procedure, the existing flora of chicken meats was suppressed by antibiotic supplements, and no suspicious colonies were detected. Likewise, CRE was not detected in DNA samples obtained from 150 chicken meat samples. Carbapenem resistance, described as the last fortress, is today's significant public health problem. According to our results, CRE was not isolated in 150 chicken meat samples offered for sale in our region.
Determining the protective effect of a boron adjuvanted vaccine with an experimental infection model in mice
(TÜBİTAK, 2023) Sayın, Zafer; Uslu, Ali; Özdemir, Özgür; Başoğlu, Abdullah; Balevi, Aslı; Uçan, Uçkun Sait; Aras, Zeki; Erganiş, Osman
Boron is a required trace element for plants, humans, and animals and it has significant influence over biological functions and the immune system. Vaccines have aided humans in the control of many contagious diseases around the world. The success of global immunization campaigns may be possible with the development of effective and low-cost vaccines and adjuvants. For this reason, the present study aimed to determine the adjuvant activity of easily accessible boron compounds. In this study, six different vaccines were prepared, including inactive Staphylococcus aureus bacteria, boric acid, aluminum hydroxide, montanide ISA 50, and ISA 206, ISA 50 + boric acid, and ISA 206 + boric acid combinations. The rate of survival following the challenge, the bacterial load on internal organs, and histopathological findings at the vaccine injection site and in the internal organs were evaluated in vaccinated mice. In addition, the levels of interferon - ? and tumor necrosis factor - ? were measured in the vaccinated seropotency groups. The rate of postchallenge mortality was 50% in the control group, and no mortality was encountered in the boric acid group. The boric acid adjuvanted vaccine decreased the bacterial load and postchallenge abscess in the internal organs and also local inflammatory reactions due to montanide adjuvants in combinations. No difference was found in the interferon - ? and tumor necrosis factor - ? levels between the control and vaccine groups. It was concluded that boric acid can be used as an adjuvant in inactivated vaccines.
Evaluation of boron’s adjuvant activity in inactive bacterin vaccines using the mice model
(Humana Press, 2021) Sayın, Zafer; Uslu, Ali; Erganiş, Osman; Başo?lu, Abdullah; Özdemir, Özgür; Sakmanoğlu, Aslı; Uçan, Uçkun Sait; Aras, Zeki
Vaccination is the most effective, reliable, and economical way of preventing or reducing the effect of infectious diseases. When preparing inactive vaccines, a range of additives called adjuvants are necessary to enhance the magnitude of the immune response. Boron has a wide range of industrial and medical applications, and its positive effects on distinct functions have been described in plants, humans, and animals. However, no studies exist about the possible adjuvant activities of boron compounds in vaccines. Hence, in this study, the potential adjuvant effect of boric acid was explored and compared with common veterinary adjuvants in a mice model. Staphylococcus aureus (S. aureus) used as vaccine antigen was isolated from dairy cows with bovine mastitis. Vaccines adjuvanted with boric acid, aluminum hydroxide, Montanide ISA 50 and ISA 206, and Montanide + boric acid combinations were prepared. The efficacy of vaccines was evaluated according to local reactions at the injection site, C-reactive protein, total Ig G, total Ig M, and anti-S. aureus antibody levels in mice. Boric acid reduced local inflammatory reactions induced by the Montanide adjuvants. Moreover, mice vaccinated with boric acid-adjuvanted vaccine had higher levels of anti-S. aureus antibody than those in the controls (P < 0.05) and were similar to the levels found in mice sensitized with aluminum hydroxide. Total Ig G and Ig M results were, however, unsuitable for the assessment of adjuvant activity for this study. In conclusion, this study revealed that boric acid has an adjuvant potential in inactive bacterin vaccines, but further target animal studies are needed.
Evaluation of fetal heart serum amyloid a concentrations in infectious cattle abortion cases
(Elsevier Ltd, 2022) Aras, Zeki; Yavuz, Orhan
Serum amyloid A (SAA) concentrations have been investigated in diseases of human and domestic animals and increased SAA levels have been reported in infectious diseases. In the present study, we determined the fetal heart blood SAA concentrations in aborted bovine fetuses and investigated the relationship between the level of SAA and causative infectious agents. A total of 46 heart blood samples were collected from aborted bovine fetuses between July 2018 and July 2019 and were assigned to two groups according to microbiological, pathological and molecular results. Group 1: An infectious disease was diagnosed by detecting a microorganism (21 cases); Group 2: An infectious or inflammatory disease was not detected (25 cases). The fetal heart blood SAA concentrations were measured by commercial ELISA test. Serum amyloid A concentrations in aborted bovine fetuses were elevated from 6.1 to ?40 mg/L in 17 of 21 cases in group 1. In group 2, SAA concentrations were less than 2.5 mg/L in 23 of 25 cases. This difference was statistically significant between group 1 and group 2. These findings suggest that SAA concentrations in fetal heart blood from bovine fetuses is potentially a novel marker for distinguishing between infectious and non-infectious bovine abortion cases.
Genotypic characterization of Bordetella bronchiseptica strains isolated from stray and pet dogs
(University of Agriculture, 2016) Sayın, Zafer; Sakmano?lu, Aslı; Erganiş, Osman; Uçan, Uçkun Sait; Hadımlı, Hasan Hüseyin; Aras, Zeki; Sanioglu, Gökçenur; Coşkun, Alparslan Aslan
Bordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. © 2016 PVJ. All rights reserved.
Identification of chlamydophila abortus in abomasum content of aborted sheep and goats by PCR
(Zoological Society Of Pakistan, 2021) Aras, Zeki; Gölen, Gökçenur Sanioğlu; Sayın, Zafer; Haydardedeoğlu, Ali Evren
Chlamydophila abortus C. abortus cause placentitis, abortion, weak neonates and infertility in sheep and goats worldwide. The aim of this study was to investigate the presence of C. abortus in abomasum content of aborted sheep and goat fetuses from provinces of the Central Anatolia of Turkey by PCR. Atotal of 105 aborted sheep fetuses and 15 aborted goat fetuses were collected from 120 different herds in the lambing seasons 2015-2016. Chlamydial DNA was detected in seven (5.8%) of the 120 abomasum content of fetuses. The 15 aborted goat fetuses were found to be negative for C. abortus. The control and vaccination programs for the fight against C. abortus infection should be determined and implemented in Turkey.
Immunohistochemical studies on infectious laryngotracheitis in the respiratory tract lesions in naturally infected laying hens
(Kafkas Üniversitesi, 2018) Terzi, Funda; Aras, Zeki; Özdemir, Özgür; Yavuz, Orhan
In this study, naturally infected by Gallid Herpesvirus type-1 in laying hens to be diagnosed by pathological and PCR methods. Sixty pieces of hens were collected in coops from Central Anatolia region. After necropsy, routine pathological processes were applied to the trachea/ larynx, sinuses, lungs and air sacs. All organs were also stained by immunoperoxidase method, and PCR methods were applied to formalin fixed paraffin embedded (FFPE) tissues. Immunohistochemically, the positivities were seen in trachea/larynx (78.3%), sinuses (61.6%), lungs (45%) and air sacs (50%). Positive reactions were observed, in mucous and gland epithelia especially located at intracytoplasmic and rarely intranuclear. PCR positivity was observed in the trachea/larynx in 15 (25%) cases, in infraorbital sinus in 11 (18.3%) cases, in lungs in 8 (13.3%) cases and in air sacs in 6 (10%) cases following the tests performed. Following these results, it is easily concluded that histopathology and immunoperoxidase method can usable for diagnosing of the ILT. However, PCR results made by FFPE tissues showed that this method is not adequate to diagnose the ILT alone.
Mycoplasma infections in dairy cattle farms in Turkey
(TÜBİTAK, 2016) Sayın, Zafer; Sakmanoğlu, Aslı; Uçan, Uçkun Sait; Uslu, Ali; Hadimli, Hasan Hüseyin; Aras, Zeki; Özdemir, Özgür; Erganiş, Osman
Mycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) isolates were identified as Mycoplasma bovis (M. bovis). Eleven (6.3%) isolates were identified as M. alkalescens, 2 (1.1%) were M. canis, and 10 (5.8%) were M. bovigenitalium. The isolation rate was found to be increasing annually. In conclusion, mycoplasmosis is a common problem in Holstein dairy cattle farms in Turkey, and M. bovis is the most frequently encountered cause of mycoplasma infections. The isolation rate seems to have increased in correlation with increased live cattle imports. Additionally, M. alkalescens and M. canis were isolated and identified in respiratory tract infections in cattle from Turkey for the first time.
Occurrence and antibiotic susceptibility of Listeria species in Turkey meats
(KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES, 2015) Aras, Zeki; Ardıç, Mustafa
The aims of this study were to investigate the occurrence of Listeria species in turkey meats and to check the antimicrobial susceptibility of the isolated strains. Hundred and fifteen raw turkey meat samples were randomly collected from the supermarkets, butchers and restaurants. Strain isolation and identification were made according to the ISO11290-1 method. Antimicrobial susceptibility was determined by the standard disc diffusion method. A total of 47 Listeria spp. were isolated from 115 (40.9%) raw turkey meat samples. The isolates were distributed between L. monocytogenes (25.53%), L. innocua (34.04%), L. grayi (31.91%) and L. welshimeri (8.51%). A total of 55.3 % of Listeria spp. isolates were multi-resistant to at least 3 of the antimicrobial agent tested. The level of multi-resistance was higher in L. monocytogenes strains (66.7%) than in L. innocua (62.5%) and L. grayi (53.3%). Listeria spp. isolates were highly resistant to ampicillin, cephalothin, penicillin, meticillin, oxacillin, and trimethoprime-sulfamethoxazole. The isolates particularly L. monocytogenes are increasingly resistant to one or more antibiotics and may represent a potential risk for public health because these antibiotics are common used in treatment of listeriosis. The correct and controlled use of antibiotics in veterinary medicine is important to the emergence of resistant strains.
Occurrence of infectious laryngotracheitis outbreaks in commercial layer hens detected by ELISA
(Taylor and Francis Inc., 2018) Aras, Zeki; Yavuz, Orhan; Sanioğlu Gölen, Gökçenur
Infectious laryngotracheitis (ILT) is an acute respiratory disease of chickens and a cause of great economic loss in commercial layers. The aims of this study were to investigate the prevalence of ILT in the field outbreaks and to compare the characteristics of ILT-infected and free flocks of commercial layers. A total of 625 blood serum samples were collected from 25 different layer flocks. The presence of antibodies against infectious laryngotracheitis virus (ILTV) in each sample was determined by ELISA. Of the 625 serum samples, 266 (42.56%) were found to be positive for ILTV antibodies. A total of 16 (64%) flocks were detected ILT positive by ELISA method. The mortality of infected flocks was statistically higher (P < 0.05) than uninfected flocks. The egg production of positive flocks was lower than that of the free flocks, but this difference was not statistically significant. The average live weight of hens in infected flocks was lower (P > 0.05) than hens in free flocks. In conclusion, the results of this study indicated a high prevalence of ILT infection in the commercial layer flocks in Konya region, Turkey. In outbreaks, ILT significantly increased the mortality rate and decreased the average live weight in layer hens. © 2018, © 2018 Taylor & Francis.
Salmonella detection in different types of packed raw poultry meat by culture elisa and pcr methods
(Parlar Scientific Publications, 2022) Aras, Zeki; Sanioğlu Gölen, Gökçenur; Kevenk, Tahsin Onur
Chicken meat is one of the most widely consumed nutrients worldwide. This study aimed to investigate the presence of Salmonella spp. in raw chicken meats by culture, ELISA, and PCR methods. A total of 200 raw chicken meat samples (80 whole chicken carcasses, 60 drumsticks, and 60 wings) were randomly collected from the butchers, supermarkets, and restaurants. The samples were analyzed by culture technique, ELISA, and PCR methods. Salmonella spp. was isolated from 34 of 200 (17 %) chicken meat samples, and 83 % were found negative. Salmonella spp. was isolated from the whole chicken carcass, drumstick, and wing samples as 15 %, 20 %, and 16.6 %, respectively. All samples were also investigated by ELISA and PCR at the same time. According to these results, Salmonella spp. was detected 13% and 16%, respectively. The high prevalence of Salmonella contamination of raw chicken meats may constitute a potential public health risk. To reduce contamination of Salmonella spp., it was stated that good manufacturing practices, good hygiene practices, and HACCP-based quality assurance systems should be applied.
Serologic prevalence of Ornithobacterium rhinotracheale infection in commercial layers
(Selçuk Üniversitesi, 2016) Aras, Zeki; Sayın, Zafer; Sanioğlu Gölen, Gökçenur
Aras Z, Sayin Z, Sanioglu G. Serologic prevalence of Ornithobacterium rhinotracheale infection in commercial layers.Aim: The purpose of this study was to determine the serological prevalence of Ornithobacterium (O.) rhinotracheale infections in commercial layers. Materials and Methods: A total of 650 blood serum samples were collected from 26 different layer flocks located in Konya, Aksaray, Karaman, Ankara, and Gaziantep provinces. None of the chickens have been vaccinated against O. rhinotracheale prior to sampling. The presence of antibodies against O. rhinotracheale in each sample was determined by ELISA. Results: Of the 650 serum samples, 113 (17.4%) were found to be positive for O. rhinotracheale antibodies. These 113 positive sera were collected from 12 (46.2%) out of 26 flocks. The twelve infected flocks belonged to 12 different farms form Konya, Gaziantep, Ankara and Karaman. Conclusion: The results of this study indicated that O. rhinotracheale infection is at high prevalence in the commercial layer flocks from this part of Turkey
Sığır abortlarında Chlamydophila abortus varlığının PZR ile araştırılması
(Selçuk Üniversitesi, 2017) Aras, Zeki; Sayın, Zafer; Sanioğlu Gölen, Gökçenur
8 species recorded so far (USTAOLU 2004, KAYA & ALTINDA 2009) out of the 461 known
Ticari yumurtacı tavuklarda Escherichia coli O157:H7 varlığının araştırılması
(2015) Aras, Zeki; Sanioğlu Gölen, Gökçenur
Bu çalışmada klinik yönden sağlıklı görünen ticari yumurtacı tavuk işletmelerinde bulunan tavuklardan toplanan kloakal sıvap örneklerinde Escherichia coli (E. coli) O157:H7 varlığının araştırılması amaçlandı. Gereç ve Yöntem: Konya ili ve çevresinde bulunan 10 farklı kümesteki yumurtacı tavuklardan 200 kloakal sıvap örneği toplandı. Tüm örnekler bakteriyolojik kültür yöntemi ile de- ğerlendirildi. İzolatların verotoksin üretimi lateral flow assay kiti ile belirlendi. Bulgular: Toplam 200 kloakal sıvap örneğinden 19 (%9.5) sorbitol negatif E. coli izole edildi. İzolatlardan 3 (%1.5) ü E. coli O157 olarak identifiye edildi ve bu suşlardan 1 (%0.5) i E. coli O157:H7 pozitif olarak bulundu. İzole edilen E. coli O157:H7 suşunun verotoksin 1 ve 2 yi ürettiği belirlendi. Öneriler: Yumurtacı tavuk kloakal sıvap örneklerinden E. coli O157 ve E. coli O157:H7 belirlendiği dikkate alındığında veteriner halk sağlığı açısından önemli olabileceği ifade edilebilir
Türkiye'de mastitisli inek sütlerinde mecC geni taşıyan metisilin dirençli Staphylococcus aureus varlığının belirlenmesi
(Selçuk Üniversitesi, 2016) Sayın, Zafer; Sakmanoğlu, Aslı; Uçan, Uçkun Sait; Pınarkara, Yasemin; Uslu, Ali; Aras, Zeki; Erganiş, Osman
Amaç: Mastitisli ineklerin süt örneklerinden izole edilen ve fenotipik olarak metisilin dirençli Staphylococcus aureus (MRSA) olduğu belirlenen izolatlarda, mecC gen varlığının araştırılması amaçlandı.Gereç ve Yöntem: Çalışma materyalini mastitisli ineklerden izole edilen 150 adet Staphylococcus aureus (S. aureus) suşu oluşturdu. İzolatlardaki metisilin direnci sefoksitin disk di-füzyon testi ve ticari PBP2a lateks aglütinasyon testi ile belir-lendi. MRSA izolatlarında mecA ve mecC gen varlığı Polime-raz Zincir Reaksiyonu (PZR) ile analiz edildi.Bulgular: Yüz elli adet S. aureus izolatından 28'inin (%18.6) sefoksitin disk difüzyon testi ile fenotipik olarak metisilin dirençli olduğu belirlendi. MRSA izolatlarının 21'inde (%75) lateks test ile aglütinasyon ve PZR ile mecA geni belirlendi. Yedi (%25) izolatta aglütinasyon ve mecA geni belirlenemez-ken, mecC geni taşıdığı tespit edildi. Öneri: Türkiye'de mastitisli ineklerde mecC MRSA izolasyon oranının beklenmedik şekilde yüksek olduğu ve fenotipik olarak MRSA olarak identifiye edilen izolatların genotipik doğrulanmasında mecC geni yönünden de analiz edilmesi gerektiği kanaatine varıldı.

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