Aksaray Üniversitesi Kurumsal Akademik Arşivi
DSpace@Aksaray, Aksaray Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Effects of Morin on the Wnt, Notch1/Hes1, KI-67/3-Nitrotyrosine and Damage Signaling Pathways in Rats Subjected to Experimental Testicular Ischemia/Reperfusion
(Springer International Publishing, 2025) Öztürk, Ayşe Betül; Şimşek, Hasan; Akaras, Nurhan; Kandemir, Fatih Mehmet
Testicular torsion, which occurs when the testicle rotates around the axis of the spermatic cord, is a serious cause of hospital admission, mostly in newborns and children, but also in adults. Oxidative stress is an important mediator of the development of complications. Morin has anti-inflammatory, anti-autophagic, and anti-apoptotic activities and especially strong antioxidant activity. This study aimed to determine the effects of Morin on testicular torsion injury. Methods: 35 Wistar rats were divided into 5 groups (n = 7): Control, Morin, I/R, I/R + MRN50, and I/R + MRN100. Parameters are effective in oxidative stress, inflammation, endoplasmic reticulum stress, apoptosis, and autophagy damage and Wnt pathway parameters, KI-67, and 3-NT levels were analyzed by biochemical, molecular, and histological methods. Results: I/R injury significantly increased oxidative stress (MDA, p < 0.001) and reduced antioxidant activity (GSH, SOD, CAT, GPx; p < 0.001). MRN administration reversed these effects, with higher doses showing greater improvement (p < 0.01 for CAT, p < 0.001 for others). Inflammation markers (NF-kB, IL-1β, TNF-α, COX-2, iNOS) were elevated in the I/R group, but MRN reduced their expression (p < 0.001). MRN also mitigated ER stress and reactivated the Wnt signaling pathway, particularly at 100 mg/kg (p < 0.001). Additionally, MRN reduced apoptosis (Caspase-3, Bax, p < 0.001) and autophagy (Beclin-1, LC3A, LC3B, p < 0.001), and improved testicular histology and sperm parameters. MRN treatment restored sperm density, motility, and viability (p < 0.05), with higher doses proving more effective. Conclusion: MRN has effects properties in testicular I/R injury by inhibiting many damage pathways and activating protective mechanisms.
Determination of the effect of Tarantula cubensis alcoholic extract on cadmium embryotoxicity
(Urmia University - Faculty of Veterinary Medicine, 2025) Canbar, Rahmi; Akçakavak, Gökhan; Uslu, Muhittin; Arslan, Mustafa Sedat; Kızılay, Harun
Tarantula cubensis alcoholic extract (TCAE) is a homeopathic product used in the veterinary field. This study aimed to determine the effects of TCAE on cadmium (Cd) toxicity in the embryo. The study used 220 fertile, incubated chicken eggs divided into 11 equal groups on the 7th day of incubation. The groups comprised untreated and physiological saline control groups, a group with TCAE alone, four groups with varying doses of Cd alone and four groups with the same doses of Cd plus TCAE. At the end of the incubation period, the eggs were opened, kidney and liver tissue samples were taken for histopathology and the number of dead and living embryos were recorded. In the prsent study, the median lethal dose of Cd was determined to be 0.029 mg per egg and the median lethal dose of Cd plus TCAE was determined to be 0.020 mg per egg. The histopathological examinations determined that kidney and liver damage were increased when TCAE and Cd were administered together, that was higher than when Cd was given alone. Thus, TCAE, which had no toxic effect on the embryo when used alone, might increase the embryotoxic activity of Cd. However, more detailed studies are needed.
Ameliorative effect of morin on diclofenac-induced testicular toxicity in rats: An investigation into different signal pathways
(Mashhad University of Medical Sciences, 2025) Şimşek, Hasan; Akaras, Nurhan; Gür, Cihan; Küçükler, Sefa; İleritürk, Mustafa; Kandemir, Fatih Mehmet
Diclofenac (Diclo) is a therapeutic agent used in the treatment of pain and inflammatory diseases, but it is also toxic to the human body. Morin is a flavonoid found naturally in plants and has many biological and pharmacological activities, including anti-inflammatory, anti-oxidant, and anticancer activities. This study aimed to investigate the efficacy of Morin in Diclo-induced testicular toxicity. Materials and Methods: Morin (50 mg/kg and 100 mg/kg) was administered orally for five days, while Diclo was administered intraperitoneally at 50 mg/kg on days 4 and 5. Biochemical, molecular, and histological methods were used to investigate oxidative stress, inflammation, apoptosis, and endoplasmic reticulum (ER) stress damage indicators in testicular tissue. Results: Morin treatment attenuated Diclo-induced oxidative stress damage by increasing anti-oxidant levels (SOD, CAT, GPx, GSH, Nrf-2, HO-1, and NQO1) and decreasing MDA levels, an indicator of lipid peroxidation. Morin reduced levels of the inflammatory mediators NF-κB protein. Increases in apoptotic Bax and Caspase-3 by Diclo were reduced by Morin, while decreased antiapoptotic Bcl-2 level was increased. Morin reduced Diclo-induced ER stress injury by decreasing ATF-6, PERK, IRE1, GRP-78, and CHOP levels. Also, Diclo decreased COX-2 levels. Conclusion: Overall, Morin may be an effective treatment of choice for testicular tissue damage associated with Diclo toxicity and may reduce the level of damage.
Effects of erythropoietin on cardiac morphometry in exercised male and female adolescent rats
(Urmia University - Faculty of Veterinary Medicine, 2025) Ateş, Mehmet Burak; Akçakavak, Gökhan; Özdemir, Özgür; Özdemir, Mehmet; Bozkurt, İbrahim
Erythropoietin (EPO) is a glycoprotein hormone predominantly produced in the kidneys, primarily stimulating erythroid cell proliferation in the bone marrow. The present study investigated the impact of EPO combined with swimming exercise on cardiac morphometry in adolescent male and female rats. The 4-week study involved 48 rats (24 males and 24 females), which were divided into four main groups of six males and six females each. The control group was administered intraperitoneal saline four times a week. The swimming exercise group also received intraperitoneal saline, followed by 30 min of swimming exercise, four times a week. The drug control group was given 50.00 IU kg-1 epoetin alfa intraperitoneally, four times a week. Lastly, the Swimming + Drug group received 50.00 IU kg-1 epoetin alfa intraperitoneally, four times a week, followed by 30 min of swimming exercise. The post-study measurements demonstrated that EPO administration did not result in notable alterations in crucial parameters, including the left ventricular mass index, left ventricular mass, and left ventricular posterior wall in the context of left ventricular hypertrophy in both genders. However, in female rats, EPO-only group and the combined EPO and exercise group showed significant thinning of the right ventricular wall and interventricular septum indicating potential cardiac dilatation. The results highlight the necessity of considering gender-specific responses when evaluating EPO's cardiovascular effects, particularly concerning the right ventricle, and suggest further investigation into the long-term consequences of these observed changes.
Use of the CHA2DS2‑VASc Score to Predict Concurrent Critical Coronary Artery Stenosis in Patients with Severe Carotid Artery Disease
(Wolters Kluwer Medknow Publications, 2025) Baran, O.; Yılmaz, Y.; İnci, S.; Çetinkaya, Z.; Keleşoğlu, S.
There is a significant correlation between the severity of atherosclerosis in one arterial region and the occurrence of atherosclerotic disease in other regions. Identifying and managing vascular disease in patients with multifocal arterial disease is often complex. The CHA2DS2-VASc score encompasses several cardiovascular risk factors and was initially used to assess the risk of thromboembolism, stroke, and death in patients with atrial fibrillation. In recent years, this score has been proposed to predict the prognosis of various cardiovascular diseases. Aim: The study aimed to investigate the prevalence of concomitant coronary artery disease (CAD) and the correlation between the CHA2DS2-VASc score and CAD in patients who were scheduled for carotid stenting due to carotid artery stenosis (CAS) but had no history of CAD. Methods: A total of 452 patients were included in the study, 213 with symptomatic CAS and 239 with asymptomatic CAS. The patients were separated into two groups: those with and without. Results: One hundred forty-eight (32.7%) of 452 patients had critical CAD. Multivariate logistic regression analysis showed that a high CHA2DS2-VASc score (OR: 4.283, 95% CI: 2.903–6.321, P < 0.001) was an independent predictor of the development of CAD. Receiver operating characteristic curve (ROC) analysis showed 64.9% sensitivity and 82% specificity in detecting CAD of the CHA2DS2-VASc score at >4 cutoff [Area under ROC curve = 0.781 (95% CI: 0.724–0.838), P < 0.001]. Conclusion: When our results were analyzed, a CHA2DS2-VASc score of >4 was highly significant in predicting severe CAD.
