Aksaray Üniversitesi Kurumsal Akademik Arşivi
DSpace@Aksaray, Aksaray Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Amphibian egg jelly as a biocompatible material: physicochemical characterization and selective cytotoxicity against melanoma cells
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Koç Bilican, Behlül; Karaduman Yeşildal, Tuğçe; Tornacı, Selay; Cansaran Duman, Demet; Öner, Ebru Toksoy; Gül, Serkan; Kaya, Murat
Extensive research on amphibians has focused on areas such as morphological and molecular taxonomy, ecology, embryology, and molecular phylogeny. However, the structure and biotechnological potential of egg jelly—which plays a protective and nutritive role for embryos—have remained largely unexplored. This study presents, for the first time, a detailed physicochemical analysis of the egg jelly of Pelophylax ridibundus, an amphibian species, using Fourier Transform Infrared Spectroscopy, Thermogravimetric Analyzer, X-ray Diffraction, and elemental analysis. The carbohydrate content was determined via High-Performance Liquid Chromatography analysis, and the protein content was identified using Liquid Chromatography-Tandem Mass Spectrometry analysis. Additionally, it was revealed that this jelly exhibits a significant cytotoxic effect on melanoma cells (viability < 30%) while showing no cytotoxicity on healthy dermal fibroblast cells (viability > 70%). Consequently, this non-toxic, biologically derived, and cultivable material is proposed as a promising candidate for cancer applications, paving the way for further research in the field.
Molecular prevalence and phylogenetic analysis of theileria ovis and theileria lestoquardi in small ruminants in Siirt province, Türkiye
(Wiley-Blackwell Publishing Ltd, 2025) Aslan Çelik, Burçak; Kara, Murat; Ayan, Adnan; Selçuk, Muhammed Ahmed; Çelik, Özgür Yaşar
Theileriosis is a haemoparasitic disease of domestic and wild ruminants, caused by Theileria species, transmitted by Ixodid tick species, particularly prevalent in tropical and subtropical regions. This study aimed to investigate the prevalence of Theileria ovis and Theileria lestoquardi in sheep and goats in Siirt province using molecular methods and to analyse their evolutionary relationships with published sequences. Materials and Methods: The animal material of the study consisted of 350 sheep and 350 goats in Siirt province and its districts. Blood samples were collected from the jugular veins of the animals and put into EDTA tubes. DNA extraction, PCR amplification and sequence analyses were performed on the collected samples. Results: According to the analysis results, the prevalence of T. ovis was found to be 5.43% (19/350, 95% confidence interval [CI] = 3.50%–8.32%) in sheep, 4.57% (16/350, 95% CI = 2.83%–7.30%) in goats and 5% (35/700) in total. A higher prevalence was determined in females (5.20%) and in the 2–3 age group (5.52%). Among sheep breeds, the highest prevalence was detected in Hamdani sheep (8.26%), while in goats, it was detected in Aleppo goats (7.27%). Among locations, the highest prevalence was determined in the Tillo district (10.34%). As a result of the research, Theileria lestoquardi was not found in either sheep or goats. Conclusion: This research confirmed the presence of T. ovis in sheep and goats in Siirt province, but T. lestoquardi was not detected. In future studies, a more detailed investigation of the presence of vector ticks and the development of regional control strategies is recommended.
Pd@SA/β-CD/Fe3O4: Pd nanoparticles on sodium alginate hydrogel beads reinforced with β-cyclodextrin and Fe3O4 particles for effective reductive elimination of organic pollutants
(Elsevier B.V., 2025) Elhüseyni, Rukiye; Çalışkan, Melike; Yılmaz Baran, Nuray; Baran, Talat
The increased use of toxic organic pollutants, such as organic dyes and nitroaromatics, in various industrial applications has led to their leak into wastewater. The inability to remove these toxic pollutants, which are non-biodegradable and stable, from wastewater poses a threat to many living organisms, from human health to the environment. Therefore, the effective removal of these pollutants from water/wastewater is crucial. In this study, we designed heterogeneous catalyst system composed of palladium nanoparticles decorated on sodium alginate (SA) hydrogel beads reinforced with β-cyclodextrin (β-CD) and Fe3O4 for the catalytic elimination of organic dyes (methylene blue (MB), methyl orange (MO), and rhodamine B (RhB)) and nitroaromatics (p-nitro-o-phenylenediamine (p-NPDA), (p-nitroaniline (p-NA), p-nitrophenol (p-NP), and o-nitroaniline (o-NA)). Morphological and structural confirmation of Pd@SA/β-CD/Fe3O4 was conducted using TEM, FE-SEM, FT-IR, EDS, BET, TG, and XRD analyses. FE-SEM and TEM analyses confirmed that the prepared Pd NPs had a spherical shape with a particle size of 10 nm, while EDS, BET, TG, FT-IR, and XRD analyses confirmed their magnetic properties and chemical structure. The catalytic capability of Pd@SA/β-CD/Fe3O4 was then evaluated for the reduction of MO, MB, RhB, p-NA, p-NP, o-NA, and p-NPDA using NaBH4, and the reductions were monitored by UV–Vis analyses. Pd@SA/β-CD/Fe3O4 with 10 nm particle size served as an effective nanocatalyst, facilitating the reduction of these contaminants within a short reaction time (0–105 s). Kinetic studies were conducted, and the rate constants (k) were determined as 0.009 s−1 for p-NP, 0.022 s−1 for o-NA, 0.012 s−1 for p-NPDA, 0.018 s−1 for p-NA, 0.054 s−1 for RhB, and 0.01 s−1 for MO. Thanks to its magnetic nature, Pd@SA/β-CD/Fe3O4 was easily recovered and reused up to six cycles, affirming its potential as a practical, economical, and sustainable catalyst. © 2025 Elsevier B.V.
The role of glutamine in growth, health and sensory quality of female rainbow trout (oncorhynchus mykiss)
(Wiley-Blackwell Publishing Ltd, 2025) Öz, Mustafa; İnanan, Burak Evren
This study aimed to investigate the effects of dietary glutamine supplementation on growth performance, haematological and biochemical responses, oxidative stress parameters and sensory quality of fillets in female rainbow trout. Methodology: A total of 360 female rainbow trout were randomly divided into four groups and fed diets supplemented with 0.0% (control), 0.5%, 1.5% and 2.0% glutamine for 60 days. Growth indices were measured, and blood samples were analysed for haematological and biochemical parameters. Muscle tissue was examined for oxidative stress markers. Sensory evaluation was performed on fillets by trained panellist. Data were analysed using one-way ANOVA and Duncan's multiple comparison test. Results: Glutamine supplementation at 1.5% and 2.0% significantly improved final body weight, weight gain and feed conversion ratio (p < 0.05). Haematological indices, such as red blood cell (RBC), haemoglobin and haematocrit levels, increased in the treated groups. Significant enhancements in total protein and albumin levels were observed, along with reduced malondialdehyde (MDA) levels, indicating improved oxidative status. Sensory evaluation revealed better odour, flavour and texture in fillets from glutamine-supplemented groups, particularly at 1.5% and 2.0% inclusion levels. Conclusions: Dietary glutamine supplementation, particularly at 1.5%–2.0%, enhances growth, physiological health, oxidative balance and sensory characteristics in female rainbow trout. These findings support the practical use of glutamine as a functional feed additive in sustainable aquaculture systems.
Evaluating the efficacy and benefits of vNOTES for ovarian cystectomy: a systematic review and meta-analysis
(Elsevier Ireland Ltd, 2025) Kaya, Cihan; Aktoz, Fatih; Güneş, Ali Can; Tercan, Can; Saçıntı, Koray Görkem; Yassa, Murat; AlAli, Hussein
This meta-analysis aims to evaluate the effectiveness and overall outcomes of vNOTES in ovarian cystectomy compared to conventional laparoscopy (CL). Study design: A systematic review and meta-analysis were conducted by searching PubMed/MEDLINE, EBSCO, Cochrane, and Embase databases for studies comparing vNOTES and laparoscopy for ovarian cystectomy. A comprehensive literature search was performed to identify relevant publications up to June 21, 2024. The search strategy utilized a combination of Medical Subject Headings (MeSH) terms and relevant keywords. Eligible studies included randomized controlled trials, cohort studies, and case-control studies, while case reports and studies lacking a direct comparison were excluded. Two independent reviewers assessed study eligibility and performed Risk of Bias evaluation. Quantitative synthesis was performed using meta-analysis techniques. The protocol was registered in the PROSPERO database (CRD42024560612). Results: Six studies reported operative times, with vNOTES showing a significantly shorter duration compared to CL (mean difference [MD]: −13.05 min, 95 % confidence interval [CI]: −19.04 to −7.07, I2 = 67 %). Five studies demonstrated that vNOTES was associated with shorter hospital stays (MD: −0.43 days, 95 % CI: −0.74 to −0.12, I2 = 90 %). Pain scores were also significantly lower in vNOTES patients (3 studies, MD: −1.25, 95 % CI: −1.49 to −1.01, I2 = 43 %). However, no significant differences were observed between the groups regarding hemoglobin drop, blood loss, or conversion rates. Conclusion: This systematic review and meta-analysis highlight the advantages of vNOTES ovarian cystectomy, including reduced operative times, faster recovery, lower postoperative pain, and shorter hospital stays. These findings support the wider adoption of vNOTES as an effective alternative to conventional laparoscopy for ovarian cystectomy, offering potential benefits for both patients and surgical efficiency.
