Anchusa Azurea var. Kurdica köklerinin farklı özütlerinin yaygın Kronik hastalıkların tedavisinde Kullanım potansiyellerinin belirlenmesi
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aksaray Üniversitesi
Erişim Hakkı
Attribution-NonCommercial 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Günümüzde yaygın olarak görülen bazı kronik hastalıkların hala bilinen bir tedavisi yoktur. Bu nedenle bu hastalıkların tedavisi için yöntem ve ilaç geliştirme çalışmaları devam etmektedir. Sentetik ilaçlar tedavide başarılı bir şekilde kullanılsa bile, bazı yan etkileri nedeniyle bu ilaç geliştirme çalışmalarında doğal ürünlerin tercih edilmesi giderek yaygınlaşmaktadır. Bu çalışma Anchusa azurea var. kurdica köklerinin antioksidan ve enzim inhibisyon aktivitelerinin belirlenerek bitkinin diyabet, Alzheimer ve kanser gibi hastalıkların tedavisinde kullanılabilme potansiyelinin ortaya konulmasına odaklanmıştır. Burada kök kısımlarından farklı çözücüler ile elde edilen özütlerinin antioksidan aktiviteleri serbest radikal giderme aktivitesi (DPPH, ABTS), FRAP, CUPRAC, fosfomolibdat ve metal şelatlama aktivite testleri ile belirlenmiştir. Enzim inhibisyon aktiviteleri kolinesteraz (asetil ve bütiril), ?-amilaz, ?-glukozidaz ve tirozinaz enzimleri için araştırılmıştır. Ayrıca özütlerin toplam fenolik ve flavonoid içerikleri yanında fenolik bileşimleri de belirlenmiştir. Antioksidan aktivite testlerinde genel olarak metanol özütü diğerlerine kıyasla yüksek aktivite gösterirken, enzim inhibisyonunda etil asetat özütü daha aktif bulunmuştur. Metanol ekstraktındaki toplam fenolik içerik 22,12 mg GAE/g ekstrakt olarak bulunurken, flavonoid içeriği tüm ekstraktlarda oldukça düşük seviyelerde bulunmuştur. Özütlerin fenolik bileşimlerinde kuersetin ve rosmarinik asit yüksek oranda bulunan fenolik bileşenler olmuştur. Elde edilen verilere göre A. azurea var. kurdica kök özütleri iyi düzeyde antioksidan ve enzim inhibisyon aktivitesi göstermiştir. Sonuçlar bitkinin kronik hastalıklarının tedavisine yönelik ilaç geliştirme çalışmalarında hammadde olarak kullanım potansiyelinin yüksek olduğunu göstermiştir
Today, some common chronic diseases still have no known treatment. For this reason, studies on method and drug development for the treatment of these diseases continue. Even if synthetic drugs are used successful in treatment, it is becoming increasingly common to prefer natural products in these drug development studies due to they have some side effects. This study focused on determining the antioxidant and enzyme inhibition activities of Anchusa azurea var. kurdica roots and revealing the plant's potential to be used in the treatment of diseases such as diabetes, Alzheimer's and cancer. Herein, antioxidant activities of extracts obtained from root parts with different solvents were determined by free radical scavenging activity (DPPH, ABTS), FRAP, CUPRAC, phosphomolybdate and metal chelation activity tests. Enzyme inhibition activities were investigated for cholinesterase (acetyl and butyryl), ?-amylase, ?-glucosidase and tyrosinase enzymes. In addition, the total phenolic and flavonoid contents of the extracts were determined as well as their phenolic compounds. In antioxidant activity tests, methanol extract generally showed higher activity compared the others, while ethyl acetate extract was found to be more active in enzyme inhibition. While the total phenolic content in the methanol extract was found at 22.12 mg GAE/g extract, the flavonoid content was found at very low levels in all extracts. Quercetin and rosmarinic acid were high amounts components in the phenolic compositions of the extracts. According to the obtained datas, A. azurea var. kurdica root extracts showed a good antioxidant and enzyme inhibition activities. The results showed that the plant has a high potential to be used as raw material for drug development studies for the treatment of chronic diseases.
Today, some common chronic diseases still have no known treatment. For this reason, studies on method and drug development for the treatment of these diseases continue. Even if synthetic drugs are used successful in treatment, it is becoming increasingly common to prefer natural products in these drug development studies due to they have some side effects. This study focused on determining the antioxidant and enzyme inhibition activities of Anchusa azurea var. kurdica roots and revealing the plant's potential to be used in the treatment of diseases such as diabetes, Alzheimer's and cancer. Herein, antioxidant activities of extracts obtained from root parts with different solvents were determined by free radical scavenging activity (DPPH, ABTS), FRAP, CUPRAC, phosphomolybdate and metal chelation activity tests. Enzyme inhibition activities were investigated for cholinesterase (acetyl and butyryl), ?-amylase, ?-glucosidase and tyrosinase enzymes. In addition, the total phenolic and flavonoid contents of the extracts were determined as well as their phenolic compounds. In antioxidant activity tests, methanol extract generally showed higher activity compared the others, while ethyl acetate extract was found to be more active in enzyme inhibition. While the total phenolic content in the methanol extract was found at 22.12 mg GAE/g extract, the flavonoid content was found at very low levels in all extracts. Quercetin and rosmarinic acid were high amounts components in the phenolic compositions of the extracts. According to the obtained datas, A. azurea var. kurdica root extracts showed a good antioxidant and enzyme inhibition activities. The results showed that the plant has a high potential to be used as raw material for drug development studies for the treatment of chronic diseases.
Açıklama
Anahtar Kelimeler
Bioactivity, Traditional Medicine, Phytochemistry, Chronic Diseases, Natural Compounds, Biyoaktivite, Geleneksel Tıp, Fitokimya, Kronik Hastalıklar, Doğal Bileşenler
Kaynak
Journal of Science and Engineering (ASUJSE)
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
1
