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    An investigation of the bactericidal and fungicidal effects of algerian propolis extracts and essential oils
    (Sivas Cumhuriyet Üniversitesi, 2022) Aşan Özüsağlam, Meltem; Tacer, Songül; Boulechfar, Safia; Zellagui, Amar
    Natural products have been used in medicine for variety of purposes for centuries. As a natural product, propolis is gaining increasing importance today due to its antimicrobial activity against pathogenic microorganisms. Hence, the aim of the present study was to investigate the antimicrobial activity of extracts and essential oils of propolis collected from various regions of Algeria against food-borne and clinically pathogen microorganisms including Salmonella enteritidis RSKK 171, Shigella sonnei MU:57 and Candida glabrata RSKK 04019. The antimicrobial activity of the propolis extracts and essential oils were evaluated using disc diffusion method. The results showed that all propolis extracts and essential oils exhibited antimicrobial activity against the tested microorganisms with inhibition zones varied from 8.31 mm to 14.53 mm. The minimal inhibitory concentration (MIC) and minimal bactericidal or fungicidal concentration (MBC or MFC) of the samples were determined by microdilution-broth method. The MIC and MBC or MFC values were in the range of 0.25-2 ?g/?l and 0.25-4 ?g/?l. Therefore, propolis extracts and essential oils from different regions of Algeria have potential to be used as a natural additive for food and pharmaceutical industries.
  • Küçük Resim
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    Anticholinesterase, anti-?-glucosidase, antioxidant and antimicrobial effects of four Algerian propolis
    (Springer, 2022) Boulechfar, Safa; Zellagui, Amar; Bensouici, Chawki; Aşan Özüsağlam, Meltem; Tacer, Songül; Hanene, Djeghim
    This study aimed to investigate the functional properties of four Algerian propolis collected from El-Menia, Oum el Bouaghi, El Harrouch and Collo regions. The total bioactive content, antioxidant, anti-enzymatic and antimicrobial effects of the four propolis methanolic extracts were evaluated using in vitro assays. The highest amount of total phenolic (561.99 ± 3.50 ?g GAE/mg E) and flavonoid content (76.98 ± 0.26 ?g QE/mg E) was found in propolis from El-Menia. Antioxidant tests (DPPH, ABTS, CUPRAC and ferric reducing power) revealed the strong scavenging and reducing abilities of the extracts. Anti-enzymatic assays against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and ?-glucosidase showed that all extracts possessed a potent inhibitory effect on ?-glucosidase better than acarbose and revealed the ability of extracts to inhibit BChE more effectively than AChE. However, the extract of propolis from El Menia was the best inhibitor of the three key enzymes with IC50 values of 11.40 ± 0.58 µg/mL 16.06 ± 0.85 µg/mL and 71.29 ± 2.73 µg/mL against ?-glucosidase, BChE and AChE, respectively. The antimicrobial assay indicated that all extracts were mainly active against Gram-positive bacteria and yeast and had bactericidal action in certain bacteria and bacteriostatic action in other ones. However, they had all fungistatic effect on C. albicans ATCC 10231. According to these results, Algerian propolis can be considered as a source of natural bioactive principles for dietary, pharmacological and medicinal applications.
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    Öğe
    Chemical composition, antioxidant, and antimicrobial activities of two essential oils from Algerian propolis
    (De Gruyter Open Ltd, 2022) Boulechfar, Safia; Zellagui, Amar; Aşan Özüsağlam, Meltem; Bensouici, Chawki; Erenler, Ramazan; Yıldız, İlyas; Tacer, Songül; Boural, Hasna; Demirtaş, İbrahim
    This study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of two essential oils (EOs) from Algerian propolis. The volatile constituents were analyzed by gas chromatography-mass spectrometry. Fifty components were identified from the oils. The major components were found to be: cedrol (17.0%), ?-eudesmol (7.7%), and ?-eudesmol (6.7%) in EO of propolis from Oum El Bouaghi (EOPO) whilst ?-pinene (56.1%), cis-verbenol (6.0%), and cyclohexene,3-acetoxy-4-(1-hydroxy-1-methylethyl)-1-methyl (4.4%) in EO of propolis from Batna (EOPB). The antioxidant properties of EOPO and EOPB were determined using 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) and cupric reducing antioxidant capacity (CUPRAC assays), respectively. Both EOs had more cupric ion reducing ability than scavenging ABTS•+ radicals. The antimicrobial potential of the two EOs against eight pathogens was assayed by the agar diffusion method and the mode of action was determined by microdilution assay. The results revealed that EOPB was bactericidal for all tested pathogenic bacteria and fungicidal for Candida albicans ATCC 10231, whereas, EOPO showed bacteriostatic effect against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC27853 and fungistatic effect against C. albicans ATCC 10231. Thus, the obtained results suggest the important use of propolis EOs as preservative agents.