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Öğe Abomazumun sola ve sağa deplasmanlarında klinoptilolit uygulaması(Yüzüncü Yıl Üniversitesi, 2018) Erdoğan, Songül; Paşa, Serdar; Erdoğan, Hasan; Alıç Ural, Deniz; Haydardedeoğlu, Ali Evren; Ural, KeremAdnan Menderes Üniversitesi, Veteriner Fakültesi, Büyük Hayvan Kliniğine 2013-2017 tarihlerinde getirilen ya da saha koşullarındaki (araştırmacıların gözetiminde, sahadaki veteriner hekimler tarafından sunulan) sol abomazum deplasmanlı (LDA) 5 ve sağ abomazum deplasmanlı (RDA) 2 inekte 1 g/kg dozda oral klinoptilolit (%93) ile sağaltım gerçekleştirildi. Uygulanma öncesinde klinoptilolit kullanılabilirliğinin uygun olduğu belirlenen ineklerde inspeksiyon, adspeksiyon, kan gazı analizlerini içeren klinik ve laboratuvar muayene gerçekleştirildi. Çalışma sonunda tüm olgularda iyileşme sağlandı ve sürüden uzaklaştırılması gereken hayvan olmadı. Hasta sahiplerinden alınan bilgilere göre klinoptilolit uygulamasından 8 hafta sonrasında 7 inekten 6’ sının gebe kaldığı öğrenildi.Öğe D-dimer levels as a procoagulative marker in association with disease progress during giardiasis in dogs(Universidad de Córdoba, 2018) Haydardedeoglu, Ali Evren; Ural, Kerem; Orman, Abdulkadir; Ural, Deniz AlicObjective. The present study was conducted to measure D-dimer concentrations and assess their value in disease activity in dogs with giardiasis. Furthermore another purpose was to analyze correlation between cyst excretion and D-dimer levels to those of dogs naturally infected with Giardia sp. Materials and methods. D-dimer analysis were performed in three groups of dogs; (i) 15 dogs with giardiasis to those of treated with secnidazol, (ii) 10 dogs with giardiasis, left untreated as control group, then were compared to those of (iii) 17dogs without giardiasis, used to detect reference ranges for D-dimer values as control group. was a correlation between D-dimer levels and logarythmic cyst counts. Results. The D-dimer range in healthy dogs was < 0.1 mg/L. In dogs with giardiasis, the D-dimer concentrations were greater than those of healthy dogs (p<0.05) and (p<0.01), respectively. The mean initial plasma D-dimer level was 2.84 +/- 0.50 and 2.99 +/- 0.61 ng/L in treated and untreated control groups. At the final follow-up evaluation on day 10 was 0.27 +/- 0.50 and 2.14 +/- 0.61 ng/L, in treated and untreated control groups, respectively, which was significantly lower in treated group (p<0.001). The area under curve (AUC) of the receiver operating characteristics for d-dimer was 0.922 (z-value = 12.977, p<0.0001). (95% CI: 0.780-0.885). At a cut-off value of 0.1 ng/L, the D-dimer measurement had a sensitivity of 87.2%, a specificity of 90.9%. Conclusions. As a result D-dimer concentrations measured in giardiasis support the probable link between probable pro-thrombotic and inflammatory condition.Öğe Determination of D-Dimer levels in calves with cryptosporidiosis(Afyon Kocatepe Üniversitesi, 2019) Cıngı, Cenker Çağrı; Kabu, Mustafa; Erdoğan, Hasan; Haydardedeoğlu, Ali Evren; Ural, KeremThe aim of the present study was emphasized to estimation of D-dimer levels among calves withcryptosporodiosis. The study was conducted on 1-3 weeks old, 11 Holstein calves with Cryptosporidiosis (studygroup) and on 1-3 weeks old ten healthy Holstein calves (control group). Diagnosis of Cryptosporidiosis made byrapid test kits. Blood samples were taken from each animal, by puncture of the jugular vein and were collectedinto plain tubes without anticoagulant. D-dimer concentrations were detected by fluorescent immunoassaytechniques in both study and control group calves. As a result, D-dimer values were significantly increased ininfected calves when compared with the control group animals.Öğe Levels of serum 25 (OH) D3 in naturally infected lambs with giardia duodenalis(Afyon Kocatepe Üniversitesi, 2019) Çamkerten, Güzin; Erdoğan, Hasan; Alıç Ural, Ali; Çamkerten, İlker; Erdoğan, Songül; Ural, KeremGiardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. Thepathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study wasto determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis.The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalisbetween 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and bothsexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthyanimals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order todetermine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopicexamination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay devicefrom the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significantchanges were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).Öğe Serum thiol disulphide levels among sheep with sarcoptic mange(Veteriner Fakultesi Dergisi, 2019) Çamkerten, İlker; Çamkerten, Güzin; Erdo?an, Hasan; Ayan, Adnan; Erdoğan, Songül; Ural, KeremSarcoptic mange, a notable parasitic disease, causes dermatological alterations among ruminants. Thiol-disulphite hemostasis is a novel oxidative stress parameter. The aim of this study was to evaluate dynamic thiol/disulfide homeostasis in sheep with sarcoptic mange. Total of thirty-six sheep (n=15 female, n=21 male) with sarcoptic mange (Group I), and twelve healthy sheep (Group II) were used in the study. A novel method was used to determine the thiol disulphide parameters. Native thiol, total thiol and Disulphide values were statistically lower in Group I. Disulphide/native thiol, Disulphide/total thiol, and Native thiol/ total thiol proportions had no statistical differences in groups. Sarcoptic mange was probably affected by the thiol Disulphide hemostasis in infected sheep. Thus, the data obtained in this study might form base for further studies to include antioxidant molecules in the treatment protocols.Öğe Stress responses of stallions during transport period: The variations of complete blood count and serum biochemistry(Atatürk Üniversitesi, 2017) Haydardedeoğlu, Ali Evren; Yağcı, Buğrahan Bekir; Ural, Kerem; Güzelbekteş, HasanIn the present study the objective was to determine the effect of one transport journey on some stress indicators in horses. 11 adult stallion (all stallion) age 3-12 years including 7 Thoroughbred, 2 Arabian, 1 Dutch Warmblood, and 1 Friesian were enrolled. White blood cell counts and other relevant parameters were determined by an automated cell counter before and after transportation. Serum activities of relevant biomarkers were determined by use of an autoanalyzer. Serum cortisole level was detected by a microplate immunoassay technique. Sodium (Na), Potassium (K) and Clor (Cl) and Calsium (Ca) levels were also measured by use of autoanalyser. Mean Platelet (PLT), and Mean corpuscular hemoglobin concentration (MCHC) White Blood Cell (WBC) counts did not exhibit a variation for healthy horses during the study whereas RBC, Hb and Hct levels exhibited significant increases after transportation (P<0.05). Mean daily glucose concentration showed an increase after transportation (P< 0.05). Mean total protein, albumine, AST and CK levels presented increases on day 2, after transportation (P< 0.05). Mean blood urea nitrogen, creatinine, total bilirubine, ALT, ALP, LDH and GGT did not show significant changes throughout the study. Mean Sodium (Na), Potassium (K) and Clor (Cl) levels showed alterations after transportation (P< 0.05), whereas Calsium (Ca) levels showed significant decreases after transportation (P< 0.05).Öğe The effct of hot-iron disbudding on Thiol-Disulphide Homeostasis in calves(Kafkas Üniversitesi, 2019) Erdoğan, Hasan; Çamkerten, İlker; Çamkerten, Güzin; Ural, Kerem; Erdoğan, Songül; Günal, İsmail; Erel, ÖzcanWe aimed to examine the effct of hot-iron disbudding on serum thiol-disulphide homeostasis levels as a marker of oxidant stress in relationship with trauma in calves under sedation, local anaesthesia, and the non-steroidal anti-inflmmatory drug ketoprofen. A total of 30 Holstein calves were enrolled in the study and allocated into three groups: disbudded following sedation with xylazine (n=10) (group I); disbudded following sedation (xylazine) and local anaesthesia with lidocaine (n=10) (group II); and disbudded after sedation (xylazine), local anaesthesia (lidocaine), and ketoprofen (n=10) (group III). Blood samples were withdrawn before (0. min) and 30, 60, 90, and 120 min after dehorning. Serum native thiols, total thiols, and disulphide levels were detected with a novel assay. Native thiol and total thiol levels were reduced in all groups without any significance during the study period. At the 90th min of the study, native thiol levels in group II were significantly lower than in groups I and III. There were no significant alterations in total thiol levels in both groups. Disulphide levels showed no significant changes in group, time, and group by time interactions, but at the 60th min, groups I and III had the lowest levels. Disulphite/ native thiol, disulphite/total thiol, and native thiol/total thiol levels had significant group alterations in the 60th min. The reduction of native thiol and total thiol levels in all groups without significance might be related to the antioxidant activity of plasma; however, it is thought that the pain management procedures should be related to the sensitive oxidative balance by thiols.Öğe The effect of hot-iron disbudding on thiol-disulphide homeostasis in calves(Kafkas Üniversitesi, 2019) Erdoğan, Hasan; Çamkerten, İlker; Çamkerten, Güzin; Ural, Kerem; Erdoğan, Songül; Günal, İsmail; Erel, ÖzcanWe aimed to examine the effect of hot-iron disbudding on serum thiol-disulphide homeostasis levels as a marker of oxidant stress in relationship with trauma in calves under sedation, local anaesthesia, and the non-steroidal anti-inflammatory drug ketoprofen. A total of 30 Holstein calves were enrolled in the study and allocated into three groups: disbudded following sedation with xylazine (n=10) (group I); disbudded following sedation (xylazine) and local anaesthesia with lidocaine (n=10) (group II); and disbudded after sedation (xylazine), local anaesthesia (lidocaine), and ketoprofen (n=10) (group Ill). Blood samples were withdrawn before (0. min) and 30, 60, 90, and 120 min after dehorning. Serum native thiols, total thiols, and disulphide levels were detected with a novel assay. Native thiol and total thiol levels were reduced in all groups without any significance during the study period. At the 90th min of the study, native thiol levels in group II were significantly lower than in groups I and Ill. There were no significant alterations in total thiol levels in both groups. Disulphide levels showed no significant changes in group, time, and group by time interactions, but at the 60m min, groups I and Ill had the lowest levels. Disulphite/native thiol, disulphite/total thiol, and native thiol/total thiol levels had significant group alterations in the 60th min. The reduction of native thiol and total thiol levels in all groups without significance might be related to the antioxidant activity of plasma; however, it is thought that the pain management procedures should be related to the sensitive oxidative balance by thiols.
