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    A long noncoding RNA lincRNA EPS expression level in renal anemia in chronic kidney disease
    (Bayrakol MP, 2025) Güner, Veysel Berk; Turgut, Faruk; Doğan, Serdar
    Chronic Kidney Disease (CKD) is a long-term medical condition characterized by the gradual loss of kidney function over time. Anemia is one of the most important causes of morbidity in patients with CKD. Anemia negatively affects the quality of life and mortality of patients and increases the progression of kidney disease. This is the first study to indicate lincRNA_EPS expression levels in CKD patients with renal anemia. Material and Methods: A total of 59 people were included in this study with 40 patients and 19 control groups. Analyzes of lncRNA expression levels were performed by RT-qPCR method. Total RNA (including miRNA and lncRNA) was obtained from serum and plasma using an RNA isolation kit. Then, RNA concentration and purity were determined spectrophotometrically. The integrity of total RNA was determined by agarose gel electrophoresis. Results: This study concluded that the lincRNA-EPS expression values were found to be significantly higher in the CKD group. There was no statistically significant correlation between the eGFR level of the patients and the lincRNA-EPS expression levels. However, a statistically significant positive correlation was found between eGFR values and hemoglobin levels in the patient group. Discussion: Considering the increased lincRNA-EPS expression level in CKD patients with renal anemia and its inverse correlation with EPO usage, a potential mechanism for facilitating erythropoiesis may involve both elevated lincRNA-EPS expression and suppression of the pro-apoptotic gene Pycard. Larger and more clinical studies are needed to fully elucidate the mechanism of action of the renal anemia-EPOlincRNA-EPS relationship. In the light of these studies, it will be possible to identify new factors that play a role in the formation mechanisms of renal anemia and to evaluate new treatment alternatives.
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    Determination of serum perfluorooctanoic acid and perfluorooctanesulfonic acid levels with different stages of chronic kidney disease
    (Wolters Kluwer Medknow Publications, 2021) Erdal, Hüseyin; Sungur, Sana; Köroğlu, Muaz; Turgut, Faruk
    The aim of this study is to investigate the perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) levels in patients with different stages of chronic kidney disease (CKD). Sixty-one CKD stage 1-4 patients who applied to the nephrology outpatient clinic were recruited. A control group consisting of 26 age- and sex-matched healthy controls were also included in the study. Concentrations of PFOA and PFOS were determined by comparing their peak areas with their standard curves. All samples were analyzed three times. The average values of blank samples were subtracted from the detected PFOA and PFOS values. PFOA and PFOS levels were significantly higher in CKD group than the controls (11.4 ± 7.47, 0.45 ± 0.55; 0.13 ± 0. 17, 0.19 ± 0.4 ng/mL, respectively) (P = 0.001). Hemoglobin, serum albumin, and estimated glomerular filtration rate (eGFR) levels were significantly lower and potassium and uric acid levels were higher in the CKD group than the controls. PFOA and PFOS levels were significantly higher in all stages of CKD patients than healthy controls. However, there was no correlation between eGFR, and PFOS and PFOA. We have demonstrated significantly increased PFOA and PFOS concentrations in different stages of CKD patients. We could not find an association between eGFR, age, and serum PFOS and PFOA concentrations.
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    Plasma Apelin Levels and Thiol/Disulfide Balance in Patients with Type 2 Diabetes Mellitus
    (Murat Demir, 2023) Gezici Güneş, Ümran; Turgut, Faruk; Erdal, Hüseyin; Doğan, Serdar
    The main objective of this research is to investigate the relationship between the plasma apelin concentrations, oxidative stress biomarkers (dynamic thiol/disulfide balance), and albuminuria in patients with type 2 diabetes mellitus. Methods: The study was carried out with 87 patients with type 2 diabetes mellitus and 24 age- and sex-matched healthy control group. Serum apelin concentrations were studied using the enzyme-linked immunosorbent assay method. Colorimetric method was used to determine native and total thiol levels. Concentrations of spot urine albumin and creatinine were measured to calculate the albumin creatinine ratio (mg/g). Results: Serum apelin concentrations were significantly lower in patients with type 2 diabetes mellitus compared to the control group (P < .001). Native and total thiol ratios were also significantly lower in diabetic patients compared to healthy patients (P < .001). The calculated disulfide levels of the patients and control groups were similar (P = .182). A negative and significant correlation was detected between serum apelin concentrations, and glucose and hemoglobin A1c levels in diabetic patients (r = ?0.272, P = .004, r = ?0.280, P = .003, respectively). A negative and significant correlation was also observed between native and total thiol levels and albumin (r = ?0.338, P = .001, r = ?0.328, P = .001, respectively). Conclusion: We found significantly lowered serum apelin concentrations and native and total thiol levels in patients with type 2 diabetes mellitus. An association was also observed between serum apelin concentrations and glycemic control. The role of apelin and thiol/disulfide balance in diabetic kidney disease requires more detailed studies.
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    The relationship between duration of hemodialysis, helicobacter pylori and CagA seropositivity in patients with chronic Kidney Disease
    (Bilimsel Tıp Yayınevi, 2020) Özcan, Oğuzhan; Erdal, Hüseyin; Özer, Burçin; Kaçmaz, Filiz; Turgut, Faruk
    Introduction: The aim of this study was to investigate the relationship between Helicobacter pylori occurrence and, Cytotoxin-associated gene A (CagA) seropositivity with hemodialysis (HD) duration, Serum C-reactive protein (CRP) and hemogram parameters in hemodialysis patients. Materials and Methods: This study included 165 prevalent HD patients. Blood samples were collected before dialysis treatment of the patients. The samples were then centrifuged at 1500 x g for 10 min. immediately after collection. The presence of serum IgG antibody for H. pylori was determined using commercial ELISA kits. Serum anti-CagA IgG antibody of H. pylori positive patients was determined by ELISA method using commercial ELISA kits. CRP levels were measured by nephelometric method, and hemogram parameters were analyzed using fully automated hematology analyzer. Results: We found that the CRP levels of the patients having H. pylori were significantly high. However, there was no significant difference between CRP levels of long-term hemodialysis (LTHD) and short -term hemodialysis (STHD) patients. The possibility of being seropositive of CagA for STHD patients having H. pylori was found to be 2.3 times higher than that of LTHD patients. In addition to that, the periodicity of gastrointestinal (GI) bleeding was significantly high in STHD patients. Conclusion: In conclusion, we found no relationship between dialysis duration and H. pylori prevalence. However, we observed significantly higher odds ratio for CagA seropositivity in patients receiving STHD. These findings suggest that longer dialysis treatment can cause improving effects on H. pylori virulence. Further studies are needed to verify these findings.
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    Thiol/disulfide homeostasis as a new oxidative stress marker in patients with Fabry disease
    (SAGE Publications Inc., 2023) Erdal, Hüseyin; Turgut, Faruk
    This is the first study to show both dynamic thiol-disulfide balance and oxidative stress levels in patients with Fabry disease (FD). This prospective study consists of 30 FD patients and 30 healthy controls. Thiol and disulfide values of the study groups were evaluated using a new, cost-effective and fully automatic colorimetric method. A total of 60 subjects were included in the study. A statistically significant difference was found between the patient and control groups for native and total thiol levels (p < 0.001). In addition, disulfide levels were significantly higher in FD patients compared with the control group (p < 0.003). Native thiol levels showed significantly negative correlation with lysosomal globotriaosylceramide, total oxidant status (TOS), and oxidative stress index (OSI) levels. In addition, a positive correlation was found between disulfide/natural thiol and disulfide/total thiol ratios and TOS, OSI, and blood urea nitrogen. We found total antioxidant status levels were lower in the patient group compared to the control group, while TOS and OSI levels were higher and were statistically significant. This study highlights for the first time a novel, cost-effective and fully automated measurement of thiol-disulfide levels in patients with FD.