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    Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages
    (Elsevıer Scıence Bv, 2018) İnanç, Muhammed Enes; Tekin, Koray; Olgaç, Kemal Tuna; Yılmaz, Burak; Çil, Beste; Taşdemir, Umut; Tuncer, Purhan Barbaros; Büyükleblebici Serhat
    The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): <= 3 years, n: 20; middle age (M): 4-6 years, n: 20; old age (O): >= 7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5 mg/120 x 10(6) CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48 h. Later, the frozen straws were thawed in a water bath for spermatological evaluation. Significant differences were observed between different age groups in terms of the spermatological parameters (p < 0.05). The evidence suggests that increasing age is associated with poor in-vitro spermatological parameters and CLC was able to protect the acrosome integrity from cryo-damage during the freeze-thawing process. Better semen freezability characteristics were obtained at young ages, considering the overall parameters.
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    Genomewide association of male reproductive traits in Aksaray Malakli dogs
    (Wiley, 2018) İnanç, Muhammed Enes; Tekin, Koray; Akkurt, Mustafa Yenal; Olgaç, Kemal Tuna; Yılmaz, Burak; Çil, Beste; Kızılaslan, Mehmet; Taşdemir, Umut; Tuncer, Purhan Barbaros; Büyükleblebici, Serhat; Uysal, Ongun; Çınar Kul, Bengi
    Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) genotype of the investigated characteristics and reproductive loci. A total of 46 male dogs are divided into three age groups (I <= 3 years, n = 19; II 4-6 years; n = 19, and III >= 7 years, n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The Affymetrix Canine 127 K SNP genotyping array v2 (Affymetrix Inc., California, USA) was used for SNP genotyping. In the primary results, the scrotal circumference was found to be higher in group II compared to other groups (p < 0.05) and the lowest total abnormal spermatozoa rate was found in group I (p < 0.05). The normal spermatozoa rate was found to be significantly above the threshold in relation to the SNP in chromosome 17. In conclusion, this study represents an exciting first step towards SNP association with dog semen spermatological parameters. Future studies might be undertaken to evaluate this SNP region for gene-knockout and expression analysis and for fine mapping to validate and/or discover the exact position of the effect region.
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    Use of infrared thermography during ejaculation process and Its link with semen quality and freezability in dogs
    (MDPI, 2021) Tekin, Koray; İnanç, Muhammed Enes; Özen, Doğukan; Çil, Beste; Olğaç, Kemal Tuna; Yılmaz, Burak; Taşdemir, Umut; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Daşkın, Ali; Uysal, Ongun; Stelletta, Calogero
    This study aimed to describe the thermal variation of external reproductive tracts during ejaculation in relation to sperm quality in dogs. Forty-six adult fertile dogs were monitored using a thermal camera before, during and after the semen collection, taking into account penile and scrotal temperatures as reproductive thermal patterns while eye and perianal temperatures were recorded as complementary thermal patterns of behavioral response. The parameters were classified depending on age (?4 years and >4 years), body weight (BW) (?75 kg and >75 kg), sperm concentration (CON) (?300 million and >300 million), total testicular volume (TTV) (?600 cm3 and >600 cm3) and total ejaculation time (TET) (?800 s and >800 s) of the animals from which semen was collected successfully. Heavier males (p < 0.05) that have more consistent testicles (p < 0.01) as well as quicker ejaculate responders (p < 0.001) and lower scrotal temperature had better semen (? motility) freezability. The lower eye temperature prior to the ejaculation (p < 0.01), lower scrotal temperature following ejaculation (p < 0.01), and conversely, higher penile temperature during the ejaculation (p < 0.001) had a higher sperm concentration. Furthermore, the sperm freezability was negatively correlated with total ejaculation time (r = -0.39, p < 0.05) and sperm abnormalities were lower in the ejaculate of dogs having a higher temperature of the scrotum, bulbus and penis. In conclusion, infrared monitoring throughout semen collection in dogs can provide information on behavioral reactions during human manipulation, as well as semen quality and testicular functionality.