Yazar "Soyocak, Ahu" seçeneğine göre listele
Listeleniyor 1 - 7 / 7
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Evaluation of relationship between SOD1 50-bp deletion gene polymorphism, Cu, Zn Level, and viscosity in postmenopausal osteoporosis patients with vertebral fractures(Humana Press, 2023) Soyocak, Ahu; Doğaner, Fulya; Ergün, Dilek Düzgün; Budak, Metin; Coşan, Didem Turgut; Özgen, MerihOxidative stress plays a role in the pathogenesis of bone loss, causing low bone mineral density (BMD) and associated osteoporotic fractures. In our study, we aimed to investigate the relationship of SOD1 50-bp insertion( Ins)/deletion( Del) polymorphism that is involved in oxidative stress metabolism, Cu and Zn element concentrations, and plasma viscosity level, with postmenopausal osteoporosis and related vertebral fractures. The study included 167 voluntary individuals. The 50- bp Ins/Del polymorphism of SOD1 was determined by allele-specific PCR. Plasma Cu and Zn levels were measured by atomic absorption spectrophotometry (AAS). The plasma viscosity was determined using the Harkness Capillary Viscometer device. In our study, the distribution of SOD1 promoter 50-bp Ins/Del polymorphism did not indicate a significant difference between the groups and in postmenopausal osteoporosis patients with and without fractures (p > 0.05). The Ins/Ins genotype was found to be common in individuals in both groups. The Cu and Zn levels of the study group were found to be between the normal reference values (p > 0.05). It was determined that plasma viscosity increased significantly in the group of osteoporotic patients and in patients with postmenopausal osteoporosis with fractures (p < 0.01). In addition, plasma viscosity was found to significantly increase in patients with Ins/Ins genotype and fractures (p < 0.01). Postmenopausal osteoporosis and associated vertebral fracture were found not to be directly related to SOD1 50-bp polymorphism and Cu and Zn element levels. Plasma viscosity levels were found to increase due to the increase in oxidative stress products. Further studies are needed to clarify the roles and relationships of SOD genes and trace elements in the development of postmenopausal osteoporosis and vertebral fracture.Öğe Genetic variation in NOD1/CARD4 and NOD2/CARD15 immune sensors and risk of osteoporosis(Portland Press Ltd, 2020) Soyocak, Ahu; Özgen, Merih; Turgut Coşan, Didem; Kurt, Hülyam; Doğaner, Fulya; Armag?n, Onur; De?irmenci, İrfan; Mutlu, Fezan ŞahinThe present study was aimed to investigate the relationship between NOD1/CARD4 and NOD2/CARD15 gene polymorphisms and osteoporosis in the Turkish population. The first time we thought that the functional polymorphisms in NOD1/CARD4 and NOD2/CARD15 genes might have triggered the development of osteoporosis. The objective of our study was to determine the relationship between NOD1/CARD4 and NOD2/CARD15 SNPs and osteoporosis. The NOD1/CARD4 (rs5743336) and NOD2/CARD15 (rs2066847) SNPs were analyzed by PCR restriction fragment length polymorphism (PCR-RFLP) in 94 healthy controls and 164 subjects with osteoporosis. PCR products were digested with restriction enzymes AvaI for NOD1/CARD4 and ApaI for NOD2/CARD15. We found that NOD1/CARD4 genotype distribution of AA, GA and GG were 15, 44 and 41% for patients and 17, 46 and 37% for controls, respectively. NOD2/CARD15 mutation was found only in three patients (1.8%) as heterozygote. The results did not show any statistical difference between NOD1/CARD4 and NOD2/CARD15 genotype distribution of patients and healthy groups (?2 = 1.740, P=0.187; ?2 = 1.311, P=0.519). However, the most frequent AG genotype (46%) of NOD1/CARD4 was observed in healthy controls, GG genotype (44%) of NOD1/CARD4 was observed as the most frequent in osteoporotic patients. NOD2/CARD15 WT/WT genotype, the most frequent genotype, was observed in both groups. Statistical analysis revealed that NOD1/CARD4 and NOD2/CARD15 polymorphisms are not associated with osteoporosis. However, a definite judgement is difficult to be made due to restricted number of patients and small size of control group. Further research is sorely warranted in this direction.Öğe Impact of tannic acid on blood pressure, oxidative stress and urinary parameters in L-NNA-induced hypertensive rats(Springer, 2015) Coşan, Didem Turgut; Saydam, Faruk; Özbayer, Cansu; Doğaner, Fulya; Soyocak, Ahu; Güneş, Hasan Veysi; Değirmenci, İrfan; Kurt, Hülyam; Üstüner, Mehmet Cengiz; Bal, CengizHypertension is a major health problem with increasing prevalence around the world. Tannic acid is water-soluble polyphenol that is present in tea, green tea, coffee, red wine, nuts, fruits and many plant foods. It has been reported to serve as an antioxidant or a pro-oxidant depending on the type of cells and its concentration. The purpose of our study was to evaluate the effect of tannic acid on systolic blood pressure, oxidative stress and some urinary parameters in the rat model of essential hypertension. Blood pressures of all rats were measured using the tail-cuff method. The nitric oxide synthase inhibitor N (omega)-nitro-L-arginine was administered orally at a dose of 0.5 g/l/day for 15 days to rats in order to create an animal model of hypertension. Tannic acid was intraperitoneally injected at a dose of 50 mg/kg for 15 days. Superoxide dismutase, catalase activity and the concentration of malondialdehyde (MDA) were determined in blood plasma and homogenates of heart, liver and kidney. In order to evaluate renal functions, urine pH, urine volume, urine creatine, uric acid, and urea nitrogen values were measured. Compared with the hypertension group, a decrease in MDA concentrations of heart tissue (p < 0.01), urea nitrogen values (p < 0.01) and urine volumes (p < 0.001) were established in hypertension + tannic acid group. There was also a decrease in blood pressure values (20th and 30th days) of this group, but there was no a statistical difference according to hypertension group. The findings of our research show the effect of tannic acid in lowering blood pressure in hypertensive rats.Öğe Is there any association between osteoporotic vertebral fracture and vitamin K epoxide reductase complex subunit-1 polymorphism in Turkish society? A pilot study(Hospital Clinicas, Sao Paul, 2019) Özgen, Merih; Coşan, Didem Turgut; Doğaner, Fulya; Soyocak, Ahu; Armağan, Onur; Kuzgun, Selen; Aydoğan, Ayşe Merve; Güneş, Hasan Veysi; Değirmenci, İrfan; Mutlu, FezanOBJECTIVE: In this study, the relationship between osteoporotic vertebral fractures and 9041 Guanine/Adenine and 3673 Guanine/Adenine polymorphisms related to the vitamin K epoxide reductase complex subunit-1 (VKORC1) gene in postmenopausal women with osteoporosis was investigated. METHOD: DNA was isolated from blood samples collected from 150 women with postmenopausal osteoporosis. Genotyping of the two polymorphic regions (9041 Guanine/Adenine and 3673 Guanine/Adenine) in VKORC1 was performed using polymerase chain reaction-restriction fragment length polymorphism analysis. The presence of radiographic fractures among the 150 patients was ascertained by using the Genant method. RESULT: At least one fracture was detected in 98 patients, and no fracture was observed in 52 patients on radiological images. We found no association between the 9041 Guanine/Adenine (p=0.283) and 3673 Guanine/Adenine (p=0.232) polymorphisms of the VKORC1 gene and the development of secondary postosteoporotic fractures in our study. CONCLUSION: There was no relationship between osteoporotic vertebral fracture and VKORC1 gene polymorphism in a postmenopausal Turkish population.Öğe Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms in Turkish postmenopausal women with osteoporosis(Taylor & Francis, 2024) Doğaner, Fulya; Soyocak, Ahu; Turgut Coşan, Didem; Özgen, Merih; Berkan, Funda; Şahin Mutlu, Fezan; Değirmenci, İrfan; Güneş, Hasan VeysiOsteoporosis is a common age-related skeletal disease, characterized by changes in the microarchitectural structure of bone tissue and decreased bone mass, especially affecting postmenopausal women. Genetic and environmental factors affecting bone metabolism play a role in the development of osteoporosis. Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme involved in the conversion of homocysteine to methionine. Genetic variations in the MTHFR gene lead to impaired function or inactivation of this enzyme. A decrease in MTHFR enzyme activity and an increase in homocysteine levels affect bone metabolism. In this study, we aimed to investigate the relationship between C677T and A1298C polymorphisms and osteoporosis in Turkish postmenopausal women. DNA samples were extracted from 200 volunteers. The PCR-RFLP technique was used to identify the MTHFR gene polymorphisms C677T and A1298C. The statistical significance of the analysis’s results was assessed. C677T genotype and allele frequency distributions were not statistically different between postmenopausal osteoporosis and healthy control groups (p=0.249, p=0.754), while A1298C genotype and allele frequency distributions were found to be statistically significant (p=0.002, p=0.013). The results of our study showed that the A1298C polymorphism may be a genetic factor associated with osteoporosis in this specific population. However, the C677T polymorphism did not show a significant connection. To gain a more comprehensive understanding of the genetic basis of osteoporosis, future research with larger sample sizes and the consideration of additional genetic and environmental factors is essential. Additionally, it is crucial to account for ethnic disparities, gene-gene interactions, and gene-environment interplays. These insights can inform the development of personalized preventive and therapeutic strategies for individuals at risk of osteoporosis in diverse populations.Öğe Postmenopozal Osteoporozda Paraoksonaz 1 L55M ve Q192R Polimorfizminin ilişkisinin değerlendirilmesi(Eskişehir Osmangazi Üniversitesi, 2020) Özgen, Merih; Turgut Coşan, Didem; Atik, Zeliha; Saydam, Faruk; Çolak, Emine; Soyocak, Ahu; Doğaner, Fulya; Berkan, Funda; Değirmenci, İrfan; Mutlu, FezanOsteoporoz, toplumda yaygın görünen, kemik mikro mimarisinde bozulma ve düşük kemik kütlesi ile karakterize sistemik bir hastalıktır. Çalışmamızın amacı paraoksonaz (PON1) geninin 584A>G(Gln192Arg) ve 172T>A(Leu55Met) polimorfizmleri ile postmenopozal osteoporoz ile ilişkisinin olup olmadığını saptamaktır. Eskişehir Osmangazi Üniversitesi Tıp Fakültesi Fiziksel Tıp ve Rehabilitasyon Anabilim Dalı Kliniği’nde takipli 143 postmenopozal osteoporozu olan ve 102 sağlıklı kadın çalışmaya dahil edildi. Çalışma retrospektif olarak planlandı. Genotipler alel-spesifik DNA primeri ve floresan özellikli DNA probu kullanılarak qPCR cihazında (The Applied Biosystems® StepOne™) belirlendi. PON1 584A>G polimorfizminin genotip ve alel frekanslarında gruplar arasında istatistiksel olarak anlamlı bir fark bulunmamıştır (p=0.362 ve p=0.318). PON1 172T>A polimorfizminin genotip ve alel frekansları karşılaştırıldığında ise, sağlıklı bireylere göre postmenopozal osteoporozu olanlarda T alelinin istatistiksel olarak anlamlı derecede yüksek bulunduğu (sırasıyla p<0.001, p<0.001) gözlendi. Çalışmamızda PON1 172T>A(Leu55Met) gen polimorfizmi ile postmenopozal osteoporoz arasında ilişki olduğu gösterildi.Öğe The association between VKORC1 gene 9041G/A polymorphism and fracture in postmenopausal osteoporosis(Elsevier Science Bv, 2014) Turgut Coşan, Didem; Doğaner, Fulya; Özgen, Merih; Soyocak, Ahu; Armağan, Onur; Guneş, Hasan Veysi; Değirmenci, İrfan; Kuzgun, Selen; Mutlu, Fezan Sahin[Abstract Not Available]
