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    Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp)
    (Elsevier Ltd., 2016) Kaya, Murat; Sofi, Karwan; Sargın, İdris; Mujtaba, Muhammad
    It is already known that chitin in a single organism can exhibit huge differences depending on the functions it serves in different body parts, but the alterations in the characteristics of chitin in course of developmental stages of an organism still remain unknown. This study presents findings on how chitin matrix is changing physicochemically through discrete morphological stages - larva, pupa and adult - of an insect (Vespa crabro). Chitin content of the organisms were found to increase gradually as the organism grew; 2.1, 6.2 and 10.3%, with a dramatic increase in chitin deposition (nearly 3 folds) during the instar from larva to pupa. Enzymatic digestion test demonstrated that chitin isolates were close to pure. Chitin isolates were also subjected to thermal pyrolysis and no variations were observed in the thermal stability of the samples. However, it was observed that surface characteristics of chitin changed greatly as the insect grew.
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    Fluctuation in physicochemical properties of chitins extracted from different body parts of honeybee
    (Elsevier, 2015) Kaya, Murat; Mujtaba, Muhammad; Bulut, Esra; Akyüz, Bahar; Zelencova, Laura; Sofi, Karwan
    It is well known that physicochemical properties of chitin are related with the extraction method. Recently, it was revealed that some physicochemical properties of chitin are also related with taxonomical relationship. For the first time in this study, it was tested how these properties of chitin are affected by different body parts of one organism. The chitins were extracted from five different body parts (head, thorax, abdomen, legs and wings) of honeybee. These chitins were physicochemically characterized and differences among these body parts were identified. Highest chitin content was observed in legs (13.25%) while the lowest from thorax (6.79%). The surface morphologies of the isolated chitin structures from five different body parts were analyzed with SEM, as a result, five different types of surface morphologies were recorded. However, three different types of surface morphologies were observed only in abdomen. Maximum degradation temperatures (DTG(max)) of thorax, abdomen, legs and wings were recorded between 359 and 367 degrees C while DTG(max) value of head chitin was found as 308 degrees C. (C) 2015 Elsevier Ltd. All rights reserved.