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    Investigation of the Effect of Serotonin on Proliferation of Adipose Tissue-Derived Mesenchymal Stem Cells via Wnt-? Signaling Pathway
    (Aksaray Üniversitesi, 2023) Semerci Sevimli, Tuğba; Demir Cevizlidere, Bahar; Ghorban, Aynaz; Gakhiyeva, Fidan; Sevimli, Murat
    Aim: Mesenchymal stem cells (MSCs) are among the most studied cells due to their properties in terms of cellular therapies. In particular, the continuity of MSCs is essential in clinical studies. The Wnt signal plays a vital role in regulating the proliferation and differentiation of MSCs. In this study, the effect of serotonin (5-hydroxytryptophan [5-HT]) on the proliferative properties of adipose tissue-derived MSCs (AD-MSCs) was investigated through the Wnt-? signaling pathway. Materials and Methods: AD-MSCs were used in the study. Firstly, the effect of serotonin concentrations ranging from 1-100 µM on AD-MSCs was analyzed by MTT test. The apoptotic effect on the cells was determined by Annexin V/PI analysis. Finally, the expression levels of WNT2, AXIN, and CTNNB1 genes were analyzed at the molecular level by qPCR. Results: The MTT analysis revealed that 75 µM serotonin reduced proliferation at 48 hours. After 48 hours of treatment with 75 µM serotonin, the apoptotic cell rate increased to 2.05%, while the early apoptotic cell rate was determined as 0.77%. When compared with the control group, WNT2, and AXIN gene expressions were up-regulated (p<0.05), while the CTNNB1 expression was down-regulated in the cells treated with serotonin (p<0.01). Conclusion: The results show that serotonin can increase the proliferative effect of mesenchymal stem cells through the Wnt signaling pathway. However, further in vivo and advanced molecular studies are required to support these findings.