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    Antibacterial, antioxidant, and angiotensin-I converting enzyme inhibition effects of margotia gummifera extracts rich in phenolic compounds
    (2022) Bouchekrit, Moufida; Laouer, Hocine; Çakmak, Yavuz Selim; Hajji, Mohamed; Boutellaa, Saber; Nasri, Moncef; Akkal, Salah
    Aromatic and medicinal plants have been used to substitute synthetic drugs with natural bioactive products. Objective: The current investigation was conducted to evaluate phenol and flavonoid contents, antibacterial, antioxidant, and anti-hypertensive potentials of three extracts of Margotia gummifera aerial parts. Methods: The antibacterial effect was assessed using the wells agar diffusion method against 11 strains. The antioxidant effect was evaluated using different methods, 2, 2-diphenyl-1-picrylhy-drazyl, total antioxidant capacity, ferric reducing ability power, cupric reducing antioxidant capaci-ty, and ?-carotene/linoleic acid bleaching assay. The anti-hypertensive activity was performed using hippuryl-histidyl-leucine substrates. Results: The highest yield, i.e., 19.801%, was obtained by the hydro-methanolic extract. However, ethyl acetate extract seemed to be rich in phenolic and flavonoid compounds compared to other ex-tracts, i.e., 822.14±69.10 mg GAE g-1 ext. and 117.28±1.80 mg RE g-1 ext., respectively. The high free radical scavenging activity was mentioned in ethyl acetate extract with IC50 of 48.820±1.25 ?g/ml. The same extract showed better antioxidant activity in all tested procedures. In antibacterial activity, the hydro-methanolic extract exhibited moderate effect against all tested bacteria except Salmonella enterica and Enterococcus faecalis which gave 18±2.0 and 16±1.0 mm of inhibition, whereas it seemed to be inactive towards two Gram-negative bacteria. Concerning anti-hyperten-sive activity, all extracts can inhibit the Angiotensin-I Converting Enzyme, but the potential effect was given by hydro-methanolic and aqueous extracts, 97.75% and 96.65%, respectively. Conclusion: It can be stated that the bioactive compounds of Margotia gummifera extracts had different biological activities, which confirm their therapeutic uses in traditional medicine.
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    Phytochemical Characterization and Assessment of Antioxidant and Anti-Alzheimer Effects of Algerian Seseli Tortuosum
    (Wiley, 2025) Slimani, Abdelkader; Akkal, Wail; Benmekhbi, Ahmed Acheraf; Mosbah, Asma; Çakmak, Yavuz Selim; Lefahal, Mostefa; Bousetla, Ahlem; Bensouici, Chawki; Laouer, Hocine; Akkal, Salah; Nieto, Gema
    HPLC analysis, phytochemical screening, thin layer chromatography, polyphenols and flavonoid contents were conducted to determine the bioactive contents of the Algerian Seseli tortuosum plant. Antioxidant activity was tested using DPPH and ABTS scavenging assays, reducing power, phenanthroline and silver nanoparticle (SNP) assays. The anti-Alzheimer's activity of extracts was also performed invitro and in silico. Phytochemical analysis highlighted the richness ofthe extracts in terms of coumarins, terpenoids and quercetin. The quantitative determination of total polyphenols and flavonoids showed that the highest amounts occurredinthe dichloromethane (DCME) and methanolic (MeOH) extracts. The antioxidant activities indicated a moderate potential. Compared with galantamine, DCME had a significantly greater anti-Alzheimer effect on BChE(CI50=9.14 +/- 1.74 mu g/ml and 34.75 +/- 1.99 mu g/ml respectively). An in silicostudy of anti-Alzheimer activity revealed a significant effect of quercetin(-30,13KJ/mol). Conclusion: This study demonstrated the richness of the phytochemical components of seseli tortuosum,which are responsible for several biological properties, mainly their anti-Alzheimer potential.