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    Invitro decontamination effect of zinc oxide nanoparticles (ZnO-NPs) on important foodborne pathogens
    (Veteriner Kontrol Merkez Araştırma Enstitüsü, 2021) Kevenk, Tahsin Onur; Koluman, Ahmet
    Zinc oxide (ZnO) has been used for many years in the pharmaceutical, cosmetic, paint, textile, and food industries for coating surfaces, absorbing UV rays and due to its antimicrobial properties in nanoscale, it has been identified as an important chemical for decontamination. Zinc can be found in many foods as well and its allowed daily intake for adults has been reported as 8-11 mg. Zinc Oxide Nanoparticles (ZnO-NPs) are generally regarded as safe (GRAS) for it being stable under hard processing conditions. Compared to organic acids, ZnO-NPs have better durability, selectivity, and heat resistance. In the present study, it was aimed to understand the decontamination effect of ZnO-NPs on S. enteritidis, S. typhimurium, S. aureus, L. monocytogenes, and E. coli O157 to develop novel, safe decontamination agents for the food industry. For this purpose, <50 µ ZnO-NPs were added into Tryptic Soy Broth in 20 mMolar final concentration for the understanding of the antimicrobial effect. After inoculation of the pathogens, a counting procedure was performed using the Tryptic Soy Agar by the pour on plate method at 0, 1st, 2nd, 4th, 6th, 8th, 12th, and 24th hours. As a result, S. enteritidis and S. aureus 3 Log CFU/mL, S. typhimurium and E. coli O157 4 Log CFU/mL, L. monocytogenes 2 Log CFU/mL decreased in 24 hours.
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    METHOD VALIDATION AND PREVALENCE OF L. Monocytogenes CONTAMINATION IN GROUND BEEF WITH VIDAS: AS AN ALTERNATIVE METHOD COMPARED WITH ISO 11290
    (Parlar Scientific Publications, 2022) Kevenk, Tahsin Onur; Koluman, Ahmet
    L. monocytogenes, which is recognized among the leading foodborne pathogen has been identified as the causative agent of listeriosis, which causes important symptoms such as abortion, septicemia, and meningitis. In the first stage of our study, the effectiveness of VIDAS LMO2 and ISO 11290:1 methods, which are frequently used today in the diagnosis of this pathogen, were compared in artificially contaminated samples. In the second stage, the presence of L. monocytogenes in ground beef samples sold in the market was revealed by both methods. According to the data achieved in the study, the relative sensitivity, specificity, accuracy, false positive, and false negative of the VIDAS LMO2 method against ISO 11290-1:2017 was found 99.236%, 73,684%, 90%, 0.263%, and 0.007% in ground beef, respectively. Although no significant difference was detected between the two methods, VIDAS LMO2 was found to be superior in terms of analysis time. Since it is known that rapid and reliable diagnosis is very important in food poisoning, the VIDAS method can be preferred more desirable due to this superiority.