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Öğe A study on the definition of some biochemical parameters and oxidative status according to milk yield in cows(TURJAF, 2021) Aksoy, Neşe Hayat; Öztürk, Caner; Karaşahin, Tahir; Dursun, ŞükrüOur study, which investigates the factors affecting milk yield at metabolic and oxidative stress levels in Holstein breed cows raised in Aksaray Region, is the first at the regional level. It was aimed to perform biochemical and antioxidant screenings according to milk yield levels in healthy Holstein cows raised in the province, and thus, together with the determination of general metabolic parameters, the differences in total antioxidant and total oxidant levels according to milk yield were investigated. Glucose, total protein, albumin, globulin, total cholesterol, triglyceride, and calcium were analysed from blood samples taken from Holstein cows that have been determined to be healthy, in milk yield age in a province’ s enterprises were analysed by using commercial test kits in a semi-analyser device. Total antioxidant and total oxidant levels were analysed spectrophotometrically with commercial kits. Statistical analysis was made with Basic Statistics Xs Display Descriptive Statistics in Minitab 16 ready-made package program. Biochemical parameters studied in sera obtained from 5 different groups from the lowest to the highest were found to be statistically significant with a positive correlation. Total oxidant level, total antioxidant level and oxidative stress indices, in which the general oxidant status was screened, were found to be correlated with milk yield in proportion to the intense metabolic and physiological status of the animals and were found to be significant. With this study, the data obtained by eliminating a deficiency in this field both contribute to the literature knowledge in the scientific area and provide useful knowledge in the field.Öğe Aksaray Malaklısı çoban köpeklerinde ehrlichia canis, anaplasma phagocytophilum, borrelia burgdorferi, dirofilara immitis enfeksiyonlarının anlık dağılımının belirlenerek hematolojik bulguların araştırılması(Harran Üniversitesi, 2019) Haydardedeoğlu, Ali Evren; Büyükleblebici, Olga; Aksoy, Neşe Hayat; Karaşahin, TahirEnfeksiyöz hastalıklar hayvan sağlığı açısından büyük önem arz etmektedir. Ekonomik değeri olan hayvanlardaki hastalıklar yanında, diğer evcil hayvanlardaki enfeksiyöz hastalıklar da hem hayvan sağlığı hem de bazılarının zoonoz olmalarından dolayı önem taşımaktadır. Evcil hayvanlar içerisinde insanla en yakın ilişkisi olan köpeklerdeki enfeksiyöz hastalıklar üzerinde yapılan çok sayıda çalışma vardır. Köpeklerde hastalığa neden olan etkenler arasında Borrelia burgdorferi, Ehrlichia canis, Anaplasma phagocytophilum ve Dirofilaria immitis önemli yer tutmaktadır. Her dört etkende kene ve sinekler aracılığıyla nakledilmekte ve vektörlüklerini yapan kene ve sinek türlerine ülkemizde sıklıkla karşılaşılmaktadır. Yaptığımız bu sero-epidemiyolojik çalışmada her dört enfeksiyon etkeni olan B. burgdorferi, E. canis, A. phagocytophilum ve D. immitis yönünden 40 olgunun negatif (-) olduğunu tespit ettik; çalışmaya aldığımız 20 erkek ve 20 dişi köpeğin daha önceden kene ile enfeste olduğu anamnezi hasta sahiplerinden alınmıştı. Köpeklerin hematolojik incelemelerinde birçoğunun kan tablosunda eozinofili olduğu görüldü. Bu dört hastalığın bölgemizde olmaması ya da subklinik olarak seyretmediği kanısı oluşmuş olmakla birlikte % 64-97 duyarlılık ile çalışan test kitlerinin kesin tanı koymaması da mümkün olabilir. Bununla birlikte hastalıkların Aksaray ilinde olmadığı da düşünülebilir.Öğe Determination of the relationship between serum anti-Mullerian hormone level and superovulatory response in Simmental cows(Wliey, 2019) Sevgi, Ramazan; Erdem, Hüseyin; Karaşahin, Tahir; Yılmaz, Mehmet Ali; Satılmış, Muharrem; Okuroğlu, Alaeddin; Ünal, İlker; Dursun, Şükrü; Alkan, Hasan; Satılmış, FatmaThe most significant focal points of the embryo transfer technology are as follows: the selection of donors, the response of the selected donor to the superovulation protocol and the obtained number of the transferable embryos. For this purpose, it is suggested that donor selection can be done by anti-Mullerian hormone (AMH) levels, and embryo production is evaluated. AMH is secreted by the granulosa cells of primordial, pre-antral and antral follicles below 4 mm in the ovary, independent of FSH. Therefore, the aim of this study was to investigate the relationship between serum AMH levels and the number of corpus luteum (CL), total embryos and transferable embryos that were shaped after a uniform superovulation protocol. For this reason, 48 Simmental cows, which were located at General Directory of Agricultural Enterprises (region, province, etc. instead of the general directorate), were used as donors for the embryo transfer. Blood samples were taken at random, regardless of the stage of animal's sexual cycle. AMH levels were measured by enzyme-linked fluorescent assay (ELFA) method of the miniVIDAS (R) (bioMerieux SA) using AMH Bovine Test Kit. According to the statistical analyses of the obtained data, AMH levels were positively correlated with CL and total embryos (p .05). No significant correlations between AMH and transferable embryos were approved (p > .05). It was also determined that each 200 pg/ml increase in serum AMH level resulted in one increase in CL number. Overall, considering the positive correlation between AMH level and the obtained number of CL and total embryos after a superovulation treatment, it was concluded that measuring blood AMH level prior to any further costly implementation may be an effective method in donor selection.Öğe Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers(Springer, 2020) Erdem, Hüseyin; Karaşahin, Tahir; Alkan, Hasan; Dursun, Şükrü; Satılmış, Fatma; Güler, MehmetThe objective of this study was to evaluate the effect of embryo quality and developmental stages on pregnancy rate in beef heifer recipients. The present study used 168 Simmental breed cows as donors, and 618 beef cattle breed heifers as recipients. The quality and developmental stages of the collected embryos were evaluated according to the criteria specified by the International Embryo Technology Society. Accordingly, the embryos in the compact morula, early blastocyst, blastocyst, and expanded blastocyst stages that were of Code I (excellent) and Code II (good) quality levels were transferred as fresh embryos to the recipient heifers. Prior to the transfer, the recipients were synchronized using the Ovsynch protocol, and the embryos obtained were transferred to 618 beef heifers. Pregnancy examinations were performed on days 30 and 60. On day 30, the pregnancy rates with Code I and Code II embryos were determined as 44.15% and 32.58%, respectively. According to the developmental stages, the pregnancy rates with Code I quality compact morula, early blastocyst, blastocyst, and expanded blastocyst were determined as 44.64%, 45.67%, 45.83%, and 33.33%, respectively. The rates of pregnancy with Code II quality compact morula, early blastocyst, and blastocyst were determined as 32.03%, 32.14%, and 50.0%, respectively. In conclusion, the pregnancy rates with Code I quality embryos were found to be higher compared with Code II embryos (P < 0.05). It was also determined that the embryonic developmental stages had no effect on the pregnancy rate (P > 0.05).Öğe Effect of flunixin meglumine treatment during and after embryo transfer on the pregnancy rate in cattle(John Wiley and Sons Inc, 2021) Karaşahin, Tahir; Alkan, Hasan; Satılmış, Fatma; Dursun, Şükrü; Erdem, HüseyinThis study aimed to determine the effect of flunixin meglumine treatment during and after the transfer of in vivo produced embryos to Angus (cows) and Holstein (cows and heifers) breeds of cattle on pregnancy rate. Holstein cows were used as donors in the study. A double dose of prostaglandin F2? was administered to the recipient animals for synchronization. Uterine flushing was performed in donors on day 7 after artificial insemination. A total of 295 transferable embryos were obtained. These embryos were transferred to Angus cows (n = 85), Holstein heifers (n = 80) and Holstein cows (n = 130). After the transfer, these animals were divided into three subgroups. The first subgroup (TI) was administered flunixin meglumine during embryo transfer, and the second subgroup (TII) was administered flunixin meglumine both during embryo transfer and on days 8 and 9 after the transfer. The third subgroup (TIII) was not administered anything and it was considered the control group. Pregnancy examination of the recipients was performed on days 30–35 after the transfer using real-time ultrasonography. The pregnancy rates after embryo transfer were found to be 43.52% in Angus cows, 42.5% in Holstein heifers, and 24.61% in Holstein cows (p <.05). When the animals were not classified according to breed, the pregnancy rates in subgroups TI, TII and TIII were found to be 29.29%, 45.10% and 29.79%, respectively (p <.05). In addition, the pregnancy rates were higher in TII and TIII subgroups of Angus cows and Holstein heifers compared to that of Holstein cows (p <.05). As a result, the pregnancy rates obtained after embryo transfer in Angus cows and Holstein heifers were found to be higher than that in Holstein cows. In addition, it was concluded that the administration of flunixin meglumine during and during/after embryo transfer has a positive effect on pregnancy rates in Angus cows and Holstein heifers.Öğe Effect of GnRH administration on different days following embryo transfer on conception rates in beef heifers(Herman Otto Intezet, 2024) Satılmış, Fatma; Erdem, Hüseyin; Karaşahin, Tahir; Aalkan, Hasan; Dursun, Şükrü; Güler, MehmetThis study investigated the effect of GnRH, administered on days 4 and 5 after the transfer of fresh embryos to beef heifers, on the conception rate. For this purpo-se, thirty Simmental cows were used as donors in the study. Progesterone-based protocol (eleven-day) was applied to the donors for estrus synchronization. In the synchronization of the recipients, the classical ovsynch protocol was applied. Uterine flushing was performed on the 7th day following artificial insemination of the donors. Code I and II quality fresh embryos were transferred to 117 recipient heifers suitable for transfer (?15 mm CL). Recipient heifers were divided into three groups; GnRH-4, GnRH-5, and control. After the embryo transfer intramuscular GnRH (10 ?g, Buserelin Acetate) was administered to the recipients on the 11th and 12th days of the cycle (4 and 5 days following the transfer). No administration was performed on heifers in the control group. On day 23 following the transfer, pregnancy examinations were performed with real-time ultrasound. Consequently, the conception rates of GnRH-4, GnRH-5, and control groups were 38.7%, 43.3%, and 33.9%, respectively (p > 0.05). As a result, GnRH administration on the 4th and 5th days following the transfer did not increase significantly the pregnancy rate. Since the pregnancy rates obtained in a high-cost biotechnological method such as embryo transfer are very important, the post-transfer GnRH administration can be recommended because there is a statistically insignificant numerical increase (approximately 10%) in the presented study. However, this strategy should be applied to a larger number of animals and be molecularly and endocrinologically evaluated.Öğe Effect of low doses of FSH and season on the in vitro maturation, fertilization and embryo development of bovine oocytes(Indian Council of Agricultural Research, 2020) Karaşahin, TahirThis study was aimed at determining the effect of follicle-stimulating hormone (FSH) and season on the in vitro maturation, fertilization and embryo development of bovine oocytes. Bovine ovaries obtained from a local slaughter house were transported to the laboratory within 2–3 h in a thermos flask containing antibiotic-supplemented physiological saline (0.9%) and at a fixed temperature of 30°C. Bovine oocytes collected in spring and autumn were incubated in culture media containing FSH at concentrations of 0.2 and 0.8 ?g/ml. After maturation, oocytes were fertilized. Fertilized oocytes were incubated in CR1aa culture medium for 7 days at 38.5°C for in vitro development. The assessment made after the completion of the maturation process revealed that, for both FSH doses, the maturation rates obtained with the oocytes collected in spring were higher than those obtained with the oocytes collected in autumn. The incubation of the oocytes collected in autumn in culture media supplemented with 0.2 ?g/ml of FSH resulted in a low level of oocyte maturation. After maturation, oocytes were subjected to fertilization. Fertilized oocytes were incubated in CR1aa culture medium for 7 days at 38.5°C for in vitro development. In both seasons, 0.8 ?g/ml FSH application was higher than the maturation values obtained with 0.2 ?g/ml FSH in terms of fertilization and embryo development rates. The study was repeated 9 times for each season. Although there was no significant difference between fertilizations and embryo development in the seasons, better results were obtained in spring season.Öğe Effects of age and sex on some hematological and biochemical parameters in Hair goats(Urmia University - Faculty of Veterinary Medicine, 2022) Karaşahin, Tahir; Aksoy, Neşe Hayat; Dursun, Şükrü; Bulut, Gaye; Haydardedeoğlu, Ali Evren; Çamkerten, Güzin; Çamkerten, İlker; İlgün, RamazanThis study was performed to determine the blood reference values of hematological and biochemical parameters in Hair goats at different ages. For this aim, clinically healthy Hair goats (n = 180, ages of six month and 1-3 years male and female) were used to collect blood samples. In whole blood, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)and red cell distribution width (RDW) were evaluated. The levels of, albumin, total protein, globulin, glucose, total bilirubin, urea, creatinine, phosphorus, magnesium, calcium, aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatine kinase (CK), alanine aminotransferase (ALT) and iron levels were investigated in sera. Age and sex had no effect on the cell structures of blood. In the either sex (male or female), age had no effect. However, sex had a significant effect on the blood cell structures except for WBC, HGB, and HCT. Albumin, protein, calcium, AST, ALT, ALP and magnesium values were not different among groups. Both the age and sex of the goats had significant effects on blood levels of glucose, phosphorus, urea, cholesterol, creatinine, GGT, CK and total bilirubin. In conclusion, this was the first study reporting the hematologic and biochemical parameters in blood of the Hair goats. The results indicated that these parameters could show variations under physiological conditions due to age and gender.Öğe Effects of glutamine on growth performance, nutrient content, fatty acid profile, and blood parameters of rainbow trout (Oncorhynchus mykiss)(John Wiley and Sons Inc, 2024) Öz, Mustafa; İnanan, Burak Evren; Karaşahin, Tahir; Dikel, SuatIn this study, different amounts of glutamine were added to the diet of rainbow trout, and they were then fed for a period of 90 days. The current research investigated the effects of glutamine on various aspects of rainbow trout, including growth performance, condition factor, viscerosomatic index, hepatosomatic index, carcass composition, fatty acid profile, hematological parameters, and biochemical parameters. The study's findings revealed that adding glutamine to the diet of rainbow trout had a beneficial impact on their growth features. The rainbow trout group that was fed a 2% concentration of glutamine experienced the most notable increase in growth rate. A statistically significant difference in growth was observed among all groups (p < 0.05). Adding glutamine to the diet increased the amount of protein and decreased the fat content in the flesh of the fish. Glutamine exerted an influence on the blood and biochemistry parameters of fish, as well as their fatty acid composition. In conclusion, the inclusion of glutamine in the diet of fish could potentially enhance their immune system, improve the quality of their muscles, and enhance their growth performance.Öğe Evaluation of the factors that affect the pregnancy rates during embryo transfer in beef heifers(Blackwell Publishing Ltd, 2020) Alkan, Hasan; Karaşahin, Tahir; Dursun, Şükrü; Erdem, Hüseyin; Satılmış, Fatma; Güler, MehmetThe aim of this study was to evaluate the effects of the transfer side, transfer location, cervix transfer score, type and diameter of corpus luteum (CL) during embryo transfer on pregnancy rates in beef heifers. Progesterone-based synchronization and superovulation protocol were applied to Simmental cows used as donors (n = 168). Uterine flushings were performed on day 7 following artificial insemination. Obtained Code I (excellent or good) and II (fair) quality embryos were transferred to recipient beef heifers (n = 561). During embryo transfer, side of transfer (right or left), transfer location (the cranial or middle third of uterine horn), cervix transfer score (easy, moderate or difficult) and type (CLa, CLb and CLc) and diameter of CL were determined. Pregnancy rates following the transfer of Code I and II embryos were 44.66% and 33.07%, respectively (p <.05). The rates of pregnancy after transfers to the right and left uterine horn were 37% and 42.2%, respectively (p >.05). The pregnancy rates were 41.2%, 34.9% and 30.3% for cervix transfer scores as easy, moderate and difficult, respectively (p >.05). Pregnancy rates after transfer to the cranial third and middle third were 41.06% and 29.67%, respectively (p <.05). According to types of CL, pregnancy rates were 31.7%, 40.4% and 45.3% for CLa, CLb and CLc, respectively (p <.05). Moreover, it was found that as the CL diameter increased, the pregnancy rates increased. As a result, it was concluded that there was no effect of side of transfer and cervix transfer score, but embryo quality, transfer location, type and diameter of CL had significant effects on the pregnancy rate during embryo transfer in beef heifers.Öğe Evaluation of the relationship between serum paraoxonase-1 activity and superovulation response/embryo yield in holstein cows(Japanese Society of Veterinary Science, 2021) Alkan, Hasan; Satılmış, Fatma; Karaşahin, Tahir; Dursun, Şükrü; Erdem, HüseyinIn this study, the effect of serum paraoxonase-1 (PON-1) activity on superovulation response and embryo yield was evaluated. The study material comprised 50 Holstein cows aged 3–4 years on postpartum day 90–120 with a body condition score of 3–3.25. A progesterone-based estrus synchronization protocol was initially administered to the selected donors. For this purpose, progesterone source was inserted intravaginally (day 0) and gonadotropin-releasing hormone injection was performed (day 6). Seven days after the insertion of progesterone device, follicle-stimulating hormone injections (total dose of 500 µg in decreasing doses for 4 days) were administered for superovulation. On the morning of the ninth day, prostaglandin (PG) F2? was administered, and the progesterone device was removed from the vagina in the evening on the same day. Two days after PGF2? administration, fixed-time artificial insemination was performed in the morning and in the evening. On the day of artificial insemination, blood samples were taken from the donors to determine the serum PON-1 activity. Uterine flushing was performed seven days after insemination. The results revealed that the serum PON-1 activity (mean ± SD, 562.71 ± 140.23 U/l) of the cows that responded to superovulation (donors with total corpus luteum count of ?3 in both ovaries) was higher than those (389.91 ± 80.51 U/l) that did not (P<0.05). On the day of insemination, a positive correlation was determined between serum PON-1 activity and the counts of total corpus luteum (r=0.398), total oocyte/embryo (r=0.468), transferable embryo (r=0.453), and Code I embryos (r=0.315, P<0.05). Unlike the Code I embryos, there was no significant correlation between serum PON-1 activity and the number of Code III embryos. Moreover, no significant difference in the number of Code III embryos between the two PON-1 groups was observed. However, embryo yield and quality were found to have increased with increased PON-1 activity. Therefore, it was concluded that serum PON-1 activity may be associated with superovulation response, embryo yield and quality in donor cows.Öğe Foliküler Kistli Holstein Irkı Düvelerde Ovsynch Protokolünün Gebelik Oranları Üzerine Etkisi(Erciyes Üniversitesi, 2023) Dursun, Şükrü; Köse, Mehmet; Kızıl, Seda Hamdi; Bulut, Gaye; Öztürk, Caner; Atlı, Mehmet; Karaşahin, TahirBu çalışmada, foliküler kistli Holstein ırkı düvelerde Ovsynch programının tedavideki etkinliğini belirlemek amaçlan- dı. Özel bir süt sığırı işletmesinde, iki veya üç tohumlamadan sonra gebe kalmayan, sık düzensiz, uzun veya sürekli östrüs belirtileri gösteren 2018 yılında 19 baş, 2022 yılında 17 baş, toplam 36 baş Holstein ırkı düve on gün arayla ikişer kez rektal palpasyon (RP) ve ultrasonografi (USG) ile tekniğine uygun olarak muayene edildi. İkinci muayeneden sonra, korpus luteum (CL) yokluğunda, 25 mm'den büyük çapta folikül taşıyan düveler (2018 yılında 13, 2022 yılında 12 olmak üzere toplam 25), foliküler kist grubuna dahil edilirken, kalan (2018’de 6, 2022 ‘de 5) 11 düve kontrol grubu olarak ayrıldı. Her iki gruptaki düvelere Ovsynch protokolü uygulandı. Düveler aynı ortamda aynı rasyon ile beslendi. Düvelerin gebelik muayeneleri tohumlamadan 45 gün sonra RP ve USG ile yapıldı. Elde edilen verilerin istatistiksel analizinde SPSS hazır paket programı kullanıldı. Gebelik oranı foliküler kist grubunda %88.0 (22/25), kontrol grubunda %72.73 (8/11) olarak tespit edildi. Grupların gebelik oranları arasında anlamlı fark yoktu (P?0.05). Sonuç olarak, Ovsynch protokolünün foliküler kisti olan düvelerde kullanılabileceği kanısına varıldı.Öğe Harmful effects of dietary supplementation of boron on blood parameters of Rainbow Trout (Oncorhynchus mykiss)(Hellenic Veterinary Medical Society, 2020) Öz, Mustafa; Karaşahin, Tahir; Aksoy, Neşe Hayat; İnanan, Burak EvrenEven though boron (B), as a trace micronutrient, occurs in natural waters and organisms, its high concentration could cause harmful and even toxic effects for organisms. Lately, studies about positive and negative effects of B on aquatic organisms have been increased with the growing scientific awareness. The aim of the present study was to determine the effects of B-containing feed (0.01%, 0.05%, 0.10%, and 0.20% of B in feed) on hematological and serum biochemical parameters of Rainbow Trout (Oncorhynchus mykiss) compared to the control feed without B. Among the most remarkable results, red blood cell, hemoglobin, and hematocrit values were dramatically decreased in the 0.20 % of B group compared to the control (P<0.05). Also, activities of liver enzymes increased with the increasing B level in the feed. Consequently, B supplementation (> 0.01%) to feed induced negative changes in blood parameters of rainbow trout.Öğe Hasak ve hasmer melez koyun ırklarına ait bazı hematolojik ve biyokimyasal parametreler(Atatürk Üniversitesi, 2015) Şimşek, Özkan; Karaşahin, Tahir; Güner, Bayram; Dursun, ŞükrüMevcut çalışma, farklı yaş aralıklarında bulunan Hasak ve Hasmer melez koyun ırklarına ait hematolojik ve biyokimyasal referans kan değerlerinin ortaya koyulması amacıyla yapıldı. Bu amaçla, klinik olarak sağlıklı oldukları belirlenen, yavru (6 ay) ve yetişkin (1-3 yaş) olmak üzere toplam 120 adet Hasak (n=60) ve Hasmer (n=60) koyunundan kan örnekleri alındı. Tam kanda, akyuvar (WBC), lenfosit (LYM), monosit (MON), granülosit (GRA), alyuvar (RBC), hemoglobin (Hb), hematokrit (PCV), ortalama alyuvar hacmi (MCV), ortalama alyuvar hemoglobini (MCH) ve ortalama alyuvar hemoglobin derişimi (MCHC) değerleri belirlendi. Serumda ise alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), gama glutamil transferaz (GGT) enzim aktiviteleri ile üre, kreatinin, albümin, glikoz, total protein ve total kolesterol düzeyleri incelendi. Her iki ırkta da, yetişkin koyunlarda LYM sayısı, AST, GGT aktiviteleri ve glikoz düzeyinin daha düşük, MCV, MCH ve total kolesterol düzeylerinin ise istatiksel olarak daha yüksek olduğu belirlendi. Bununla birlikte, diğer hematolojik ve biyokimyasal parametrelerde, yaşa bağlı istatiksel olarak önemli bir fark bulunmadı. Sonuç olarak, bu çalışmadan elde edilen verilerin, Hasak ve Hasmer melez koyun ırkları için referans kan değerleri olarak kabul edilebileceği düşünülmektedir.Öğe Investigation of bull effect on in vitro embryo production(Kafkas Üniversitesi, 2014) Akyol, Numan; Kızıl, Sedat Hamdi; Satılmış, Muharrem; Karaşahin, TahirThe aim of the study was to show whether there were some differences among 9 Holstein bulls and within their replications on their ability of in vitro fertilization for in vitro embryo production as cleavage and coming into the blastocyst stage. Semen collected and frozen from nine Holstein bulls with satisfactory in vivo fertilization capabilities for artificial insemination was used for in vitro fertilization. Direct washing method by Brackett and Oliphant medium and 5 or 6 h incubation period were used for in vitro fertilization. Charles Rosenkrans medium was used for in vitro embryo culture. An atmosphere with a higher than 95% relative humidity, 39°C, 5% CO2 and was used for all in vitro embryo production processes. Totally 2519 A and B quality oocytes were treated for in vitro embryo production. As a result statistically significant (P0.05) between the replications for each bull as cleavage and coming into blastocyst stage. The results showed varied capabilities of bulls for in vitro fertilization and embryo production and male factor can affect success of in vitro embryo productionÖğe Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos(TÜBİTAK, 2014) Karaşahin, Tahir; Akyol, Numan; Satılmış, Muharrem; Bucak, Mustafa Numan; Çoyan, KenanThe objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Sevenday-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively. The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%.Öğe Relationship between total antioxidant/oxidant status, and oxidative stress index and superovulation response in donor cows(Elsevier, 2021) Karaşahin, Tahir; Alkan, Hasan; Satılmış, Fatma; Dursun, Şükrü; Öztürk, Caner; Bulut, Gaye; Aksoy, Neşe Hayat; Tekindal, Mustafa Agah; Çağlayan, Tamer; Yeşilkaya, Ömer Faruk; Erdem, HüseyinThe aim of the present study was to determine the effects of serum total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) on counts of corpus luteum, transferable embryo, and embryo quality in cows treated with superovulation protocol. A total of 60 Simmental cows (postpartum 90 to 120 days) were used. The donors were treated with a 9 day-progesterone based estrus synchronization protocol. On the 7th day of the progesterone treatment, donors were treated with FSH twice daily as decreasing doses for 4 days. On day 9, PGF 2 injection was performed in the morning and progesterone source was removed in the evening. The cows were inseminated twice artificially on day 11 at 12 hours interval. On the day of artificial insemination, blood samples were collected to determine serum TAS, TOS and OSI concentrations. Uterine flushing was performed on day 7 after artificial insemination. Serum TAS, TOS and OSI concentrations of cows were 1.62 mu mol/L, 5.44 mu mol/L and 0.45 AU, respectively. There was a positive correlation between TAS concentration and the counts of transferable embryo, Code I and II quality embryo (P<0.05). TOS and OSI concentrations were negatively correlated with the counts of transferable embryo and Code I embryo, and positively correlated with unfertilized oocyte counts (P<0.05). As a result, it was concluded that TAS, TOS, and OSI affected the counts of transferable embryo, embryo quality, and unfertilized oocyte in donor cows.Öğe Serum cholesterol levels in hair goats of Aksaray Region(Agricultural Research Communication Centre, 2019) Karaşahin, Tahir; Aksoy, Neşe Hayat; Haydardedeoğlu, Ali Evren; Dursun, Şükrü; Bulut, Gaye; Çamkerten, Güzin; Çamkerten, İlker; İlgün, RamazanThis study was conducted to determine the levels of serum total cholesterol in Hair goats in different gender and age ranges. For this purpose, the blood samples from 120 Hair goats, young (<= 6 months) and adult (2-4 years), which were found to be clinically healthy, were taken from the vena jugularis. Triglyceride, total cholesterol, HDL and LDL cholesterol levels were measured in sera samples. The mean values in adult female goats, young female goats, adult male goats and young male goats were found. Triglyceride levels were found to be higher in male goats than in females. It was observed that the HDL values obtained from male kids were lower than the other ones. The LDL results obtained from the goats were close to each other and statistically insignificant. Thus, in this study, the data obtained is considered to contribute to the formation of reference values in the Hair goats.Öğe The effect of oleic and linoleic acids on in vitro bovineembryonic development and embryo quality(Turkish Journal of Veterinary and Animal Sciences, 2015) Karaşahin, Tahir; Arıkan, ŞevketThe present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morulablastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.Öğe The effects of low doses of FSH and season on the in vitro maturation of bovine oocytes(Wiley-Blackwell, 2015) Karaşahin, Tahir; Arıkan, Şevket[Abstract Not Available]
