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Öğe Comparison of the effects of Autologous Cytokine-rich Serum (ACRS) and Platelet-Rich Plasma (PRP) on skin wound healing(Sciendo, 2025) Erol, Hanifi; Karakaş, Erol; Ermiş, Mustafa; Akçakavak, Gökhan; Aslan, Nevzat Emre; Karataş, ÖzhanWound healing is one of the most complex biological events, involving physiological processes such as tissue restoration and intricate cellular and molecular activities. The aim of this study was to investigate the effects of Autologous Cytokine Rich Serum (ACRS) and Platelet-rich plasma (PRP) on wound healing and to compare their impact on tissue repair using histopathological and immunohistochemical methods. A total of 42 healthy Wistar-Albino rats were used as material. The histopathological and immunohistochemical evaluations showed that ACRS is more effective than PRP on wound healing. The superior efficacy of ACRS is attributed to its stimulation of anti-inflammatory cytokines and provision of essential nutrients such as amino acids, vitamins, and lipids. Further detailed studies are recommended to explore these findings.Öğe Comparison of thermographic imaging and other diagnostic techniques in diagnosis of cattle with laminitis(Universidade Federal do Rio Grande do Sul, 2018) Arıcan, Mustafa; Hatipo?lu, Fatih; Erol, Hanifi; Kanat, Özgür; Yavuz, Orhan; Parlak, Kurtuluş; Koç, OsmanBackground: Lameness results in major economic losses on dairy farms. The proportion of lameness in cows are between 4-55%, the origin of 90% lameness are caused by foot diseases. Hoof abnormalities such as laminitis are major contributors to lameness. Aim of the study, compared with thermographic imaging (IRT) and radiographic, magnetic resonans (MR), computer tomography (CT), histopathological analysis on the diagnosis of laminitis. Materials, Methods & Results: Preliminary, the animals (20 Holstein-Fresian) were selected by lameness examination for evaluation of the for any obvious signs of disease. The animals were examined when rising and walking of the free stalls. Walking was very painful and clinical symptoms of general distress become present for laminitic group. The cows were scored on a 1 to 5 scale for their locomotion (1 = normal locomotion; 5 = severely lame). Thermographic examinations were performed the all animals after the routine clinical examination methods. The infrared images were taken from the dorsal view of all hooves to monitor the temperature of coronary band. All images were scanned using a hand-held portable infrared camera (Wahl, Thermal Imager HSI3000 Series), which was calibrated to ambient temperature and absorptive conditions. Incentives radiological examinations, dorso-palmar and latero-medial shooting was done for using of (Regius Model 110 Konica, Minolta) 70 kV and 30 mAs for each claws. MRI images of all isolated digits were performed at the Department of Radiology, by using Siemens Symphony Erlangen, Germany. Continuous series of transversal, sagital and dorsoplantar scan were obtained from all digit. Computer tomography (CT), isolated digits were performed at the Imaging Department of Radiology, by using Siemens Symphony Erlangen, Germany. CT scanner was used to obtain contiguous slices from the region of the proximal sesamoid bones to the toe. Anterior side (laminar and perilaminar tissues) of the claws was uncovered with electric wheel. Tissue samples were taken 10% buffered formaldehyde solution after establishing a routine laboratory procedure following the prepared paraffin blocks 5 micrometers thick sections with hematoxylineosin dye method according to the staining. It was finally examined by light microscopy. The computer soft ware of the infrared thermal camera showed increased local temperature, by 0.5-1.5°C, between the normal and suspected regions for laminitic cases. Clinically, animals suspected of laminitis were showed general stiffness, lameness during walking difficulty. Examination of the claws was demonstrated of rings and plump appearance was evident in paries ungulae. The main damage was seen during trimming of the sole from no hemorrhages or discoloration of control group. The transversal MRI images provided excellent depictions of anatomical structures when compared to their corresponding pictures. Identifiable anatomic structure was labeled on the line drawings of the limb sections and on the corresponding CT images. Twenty out of 30 claws (65%) were shown laminitis statistical with histopathological examination. In histopathological examination; mononuclear cells and neutrophil granulocytes infilrations and edema, haemorrhagia, mild hyperemia in the lamellar region was remarkable in laminitic cases. Discussion: As a results, thermographic examination may have potential as a detection tool for laminitis. MR transversal images provided excellent depiction of anatomical structures and many biometric research in the bovine hoof can be easily investigated. The usefulness of IRT, MRI, CT in evaluating laminitis in the acute patient remains stil open. The use of diagnostic techniques during the initial active phase of laminitis as a means to increase the understanding of the disease and also serves as a justification for the development of an experiment involving live cows induces laminitis.Öğe Effect of autologous cytokine-rich serum and platelet-rich plasma administration on oxidative status, minerals and proinflammatory cytokines in brain and serum in cyclophosphamide-induced ovarian failure(Springer Science and Business Media B.V., 2025) Ermiş, Mustafa; Karakaş, Erol; Erol, Hanifi; Akçakavak, Gökhan; Acı, Recai; Ümit, Furkan; Karataş, Özhan; Çiftçi, GülayCyclophosphamide (CP) is one of the most commonly used chemotherapy agents and carries a high risk of ovarian damage. This study aimed to evaluate the effects of autologous cytokine-rich serum (ACRS) and platelet-rich plasma (PRP) on brain oxidative status, mineral levels, and proinflammatory cytokines in rats with CP-induced ovarian failure. A total of 42 female Wistar rats (12-weeks-old) were used in the study. Six of these rats were allocated as donors, and the remaining 36 rats were randomly distributed into six groups (n = 6 per group). Group 1 received no treatment. On the 1st and 7th days, 75 mg/kg of CP was administered intraperitoneally to Groups 4, 5, and 6. On day 1, PRP was administered intraovarianly to Groups 2 and 5, while ACRS was administered intraovarianly to Groups 3 and 6. Additionally, PRP and ACRS were administered intraperitoneally to the respective groups on 7th and 14th days.The study was terminated at the end of the 31st day. Brain tissue and blood samples were collected for biochemical analyses and ovarian tissue samples were collected for histomorphological examinations. Morphological analysis using Hematoxylin–Eosin (HE) staining and immunohistochemical evaluation for AMH, α-SMA, and IL-1β were conducted on the ovaries. Proinflammatory cytokines and insulin levels were measured using ELISA test kits. TAS/TOS levels were assessed using Relassay Diagnostic kits. Biochemical parameters and mineral levels were measured using autoanalyzer. Histopathological evaluation revealed that follicular degeneration, congestion, hemorrhage, edema, and inflammatory cell infiltration, as well as the number of atretic follicles and IL-1β immunoreactivity, were observed at the highest levels in the CP group (Group 4). In contrast, the numbers of primordial, primary, secondary, and tertiary follicles, along with AMH and α-SMA immunoreactivity levels, were found to be the lowest in this group. However, positive therapeutic effects were observed in the CP-treated groups (Groups 5 and 6). In the serum, increased levels of AST, ALT, creatinine, glucose, LDL, TOS, Ca, Fe, Mg, IL-1β, IL-1α, TNF-α, and NF-kB were detected in the CP groups (G4, G5, G6) compared to the control groups (G1, G2, and G3). In brain tissue, a decrease of total protein and total cholesterol levels were observed in the CP groups (G4, G5, G6) compared to the control groups, while increases in Na, Cl, Fe, IL-1β, IL-1α, TNF-α, and NF-kB levels were detected. In conclusion, PRP and ACRS therapies from the patient's own blood have a potential as supportive or chemopreventive strategies with reduced side effects and treatment costs.Öğe Investigation of the Effect of Autologous Cytokine Rich Serum (ACRS) and Platelet Rich Plasma (PRP) Treatment on Experimental Uterine Horn Peritoneal Adhesion Model in Rats(Springer International Publishing, 2025) Erol, Hanifi; Karakaş, Erol; Akçakavak, Gökhan; Aslan, Nevzat Emre; Karataş, ÖzhanAdhesion formation is a complex biological process defined as adhesion of intra-abdominal organs to one another or to the abdominal wall with fibrous bands. In recent years, various methods and drugs have been used for preventing the abdominal adhesion. Autologous cytokine rich serum (ACRS) is a blood-derived product obtained by incubating and centrifuging whole blood. It contains various cytokines and factors. Platelet rich plasma (PRP) is a blood product and rich in growth factors. Objective: The aim of this study is to compare and investigate the effects of PRP and ACRS on uterine horn peritoneal adhesion. Methods: The animal material of the study was consisted 42 healthy, 4 months old, non-pregnant female Wistar-Albino rats with 150–200 g, body weight. While 6 of these 42 animals included in the study were used as donors to PRP and ACRS, 36 animals were divided into six groups with 6 rats in each group. Result: Adhesion, inflammatory cell infiltration, TNF-α, IL-6, Col-I, α-SMA scores were compared between groups and ACRS group’s scores were significantly lower than other groups. Conclusion: As result of this study, ACRS treatment has shown to provide more effective manipulation of the inflammatory response. Especially, ACRS treatment has more effective anti-inflammatory effect on peritoneal adhesion was seen with the histopathologic findings of this study. Our current findings suggest that ACRS has an anti-adhesion role by reducing the inflammatory response in adhesion tissues.
