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Öğe Antimicrobial and antioxidant activity of various solvent extracts of Salsola stenoptera wagenitz and Petrosimonia nigdeensis aellen (Chenopodiaceae) plants(Chiang Mai University, 2015) Aşan Özüsağlam, Meltem; Erzengin, Mahmut; Darılmaz, Derya Önal; Erkul, Seher Karaman; Tekşen, Mehtap; Karakoca, KübraThis study was conducted to examine the in vitro antimicrobial and antioxidant activities of various extracts (ethanol, methanol, n-hexane, dicholoromethane and water) prepared from the stem and leaf+fruit of Salsola stenoptera Wagenitz and Petrosimonia nigdeensis Aellen (Chenopodiaceae) plants. The antimicrobial activities of the plant extracts were tested according to agar well diffusion and microdilution broth methods. The extracts showed a strong antimicrobial activity and a broad antimicrobial spectrum for all tested microorganisms. Minimal bactericidal (MBC) or fungicidal (MFC) concentrations for microorganisms which were sensitive to the stem and fruit+leaf extracts of S. stenoptera and P. nigdeensis in the agar well diffusion assay were in the range of 5.63-180.00 mg/mL and 11.25-180.00 mg/mL, respectively. The extracts were also subjected to screening for their possible antioxidant activity with scavenging activity of DPPH radicals, hydrogen peroxide scavenging activity and chelating ability on ferrous ions. All tested extracts showed varying degrees of efficacy in each assay in a dose-dependent manner. In general, polar extracts exhibited stronger activities than non-polar extracts. The ethanol and n-hexane extracts of S. stenoptera and P. nigdeensis stem showed the highest scavenging abilitiy on DPPH radicals with the IC50 values of 29.84 µg/mL and 83.56 µg/mL, respectively. The total phenolic and flavonoid contents were also determined. The highest gallic acid equivalents (GAE) and quercetin equivalents (QE) values, for both plants, were 44.78 µg GAE/mg and 66.69 µg QE/mg, respectively. The results indicate that both plant species may have health benefits as sources of natural antioxidants and antimicrobial agents for use in functional foods and/or in pharmaceutical industry. © 2015, Chiang Mai University. All rights reserved.Öğe Antioxidative, antimicrobial and cytotoxic properties of Isatis floribunda Boiss. ex Bornm. extracts(2013) Karakoca, Kübra; Aşan Özüsağlam, Meltem; Çakmak, Yavuz Selim; Erkul, Seher KaramanThe Isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of Anatolian-Turkey. In the present study, the antioxidant activities of Isatis floribunda Boiss. ex Bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. The total phenolic compounds and flavonoids were also examined for the extracts. The antimicrobial activities of the extracts were investigated by using the disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The phenolic composition of I. floribunda root methanolic extract was analyzed by high performance liquid chromatography (HPLC). The major component in the extract was cholorogenic acid (1980.20 µg/g). The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.Öğe Karyological studies on Oxytropis (Fabaceae) from Turkey(UNIV FLORENCE BOTANY INST, 2015) Martin, Esra; Erkul, Seher Karaman; Aytaç, ZekiThe karyological analyses of 11 species, one taxon of which is endemic of Oxytropis (Fabaceae), distributed in Turkey were examined in this study. Among these taxa, the chromosome numbers were as follows: 2n = 16 in Oxytropis kotschyana, O. pallasii, O. pilosa, O. savellanica, O. persica, O. albana, O. argyroleuca, O. aucheri, O. karjaginii, O. lupinoides; and 2n = 96 in O. lazica. The karyotype analysis of taxa belonging to the genus Oxytropis was performed using Image Analysis System (Bs200Pro).Öğe Production and characterization of chitosan based edible films from Berberis crataegina's fruit extract and seed oil(Elsevier, 2018) Kaya, Murat; Ravikumar, Preethi; İlk, Sedef; Mujtaba, Muhammad; Akyüz, Lalehan; Labidi, Jalel; Salaberria, Asier M.; Çakmak, Yavuz S.; Erkul, Seher KaramanChitosan-based edible films were prepared by supplementing Berberis crataegina DC.'s seed oil and fruit extract into chitosan matrix. The produced films have characterized both physiochemically (SEM, DSC, FT-IR, UV-vis, contact angle and mechanical analysis) and biologically (anti-quorum sensing, antimicrobial and antioxidant). Chitosan-fruit extract film revealed higher thermal stability, antioxidant, antimicrobial and anti-quorum sensing activity compared to other films. Addition of B. crataegina's seed oil and fruit extract into the chitosan film notably decreased the UV-vis transmittance but ameliorate the tensile strength values. Hydrophobicity of the chitosan-seed oil film was observed to be higher (92.64 +/- 4.17) than chitosan-control film (84.67 +/- 1.50) while chitosan-fruit extract film exhibited slightly lower hydrophobicity (73.82 +/- 7.42) than chitosan film. The overall high thermal stability, antioxidant and antimicrobial activity of chitosan-fruit extract film revealed that B. crataegina's fruit extract can be used as an effective ingredient for the production of the edible film with enhanced physicochemical and biological properties.Öğe Ranunculus aydogdui (Ranunculaceae), a new species from Turkey(Wiley, 2021) Erkul, Seher Karaman; Tekşen, Mehtap; Duman, HayriRanunculus aydogdui sp. nov. is described from Tuz Golu (Salt Lake), Aksaray, Turkey. It is compared to three similar species, R. flammula, R. pulchellus var. pulchellus and R. indivisus and it is found that R. aydogdui is distinguished from them e.g. by having tuber roots. A comprehensive description, ecology, distribution map, detailed photographs, pollen morphology and comparison of the new species with closely related species are provided. The new species is assessed as Critically Endangered (CR) following IUCN Red List Categories and Criteria.Öğe The in vitro antimicrobial and antioxidant activities of various extracts of endemic Salsola Stenoptera Wagenitz (Chenopodiaceae)(Current Biology, 2011) Aşan Özüsağlam, Meltem; Erzengin, Mahmut; Darılmaz, Derya Önal; Erkul, Seher Karaman; Teksen, Mehtap; Karakoca, Kubra[Abstract Not Available]
