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Öğe Innovation of strategies and challenges for fungal nanobiotechnology(Springer, 2016) Sağlam, Necdet; Yeşilada, Özfer; Çabuk, Ahmet; Şam, Mesut; Sagğam, Semran; İlk, Sedef; Emül, Ezgi; Çelik, Pınar Aytar; Gürel, Ekrem; Prasad, RNanotechnology involves the study and use of materials under the 100 nm scale, exploiting the different physiochemical properties exhibited by these materials at the nanoscale level. Microorganisms are the best model and role of action for the nano/biotechnological applications. This technology has become increasingly important for the biotechnology and the related sectors. Promising applications have been already employed in the areas of drug delivery systems using bioactive nanoencapsulation, biosensors to detect and quantify pathogens, chemical and organic compounds, alteration of food compositions, and high-performance sensors and film to preserve fruits and vegetables. Moreover, the taste of food and food safety can be improved by new nano-materials from the microbiological sources. The huge benefits from this technology have led to increases in the market investments in nanoscience and nanoproducts in several areas.Öğe Lantibiotics nanotechnology, bioengineering, and biotechnology(Springer Science and Business Media B.V., 2021) Şam, Mesut; Sağlam, Semran; Altındağ, Serap; Köse, Kazım; Emül, Ezgi; Uzun, Lokman; Sağlam, NecdetThe term “antibiotics” refers to antibacterial agents synthesized by certain lactic bacteria. These microorganisms produce antibiotics to prevent pathogens from settling in the same environment and to prevent pathogens’ nourishment. Lantibiotics are antimicrobial peptides produced by most gram-negative and gram-positive bacteria. These bacteriosins are called LANTIBIOTIC and present themselves as an interesting addition to the “traditional” antimicrobials when the use of antibiotic resistance is compromised. Bacteriocins are antimicrobial components of protein structure that are synthesized and secreted by bacteria. Inhibition effects are observed mostly on neighbor species. Although there are many varieties with different properties, nisin, which is obtained from lactic acid bacteria, is safely and widely used in foods. Thus, knowing the properties of other bacteriocins along with nisin may enable the effective use of these antimicrobial agents in many food products.Öğe Synthesis and characterization of silver nanoparticles integrated in polyvinyl alcohol nanofibers for bionanotechnological applications(TÜBİTAK, 2016) Tan, Gamze; Sağlam, Semran; Emül, Ezgi; Erdönmez, Demet; Sağlam, NecdetCharacterization and microbial growth activity of polyvinyl alcohol (PVA) nanofibers (NFs) produced by an electrospinning technique containing different amounts of silver nanoparticles (AgNPs) was investigated. AgNPs were synthesized by chemical reduction of silver nitrate (AgNO3 ) (in different weight ratios) in PVA solution followed by electrospinning to produce PVA/AgNPs NFs. The properties of PVA/AgNPs NFs were studied as a function of the silver content. The morphology and distribution of NFs and AgNPs were characterized by transmission and scanning electron microscopy. The size of NFs was 135–160 nm, and the size of AgNPs was 15–27 nm. TEM images clearly showed that spherical AgNPs were homogeneously integrated in PVA NFs. The microbial activity of NFs containing different amounts of AgNPs was examined against both Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Bacillus megaterium and Staphylococcus aureus) bacteria and against Candida albicans. PVA NFs containing AgNPs exhibited increasing inhibitory effects against microbial pathogenic strains with increasing AgNPs amounts. The results suggest that PVA/AgNPs NFs could be used for wound dressing components, protective coatings, biomedical devices, and water purification.