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    The effect of different additives on semen freezing of Aksaray Malaklı shepherd dog
    (Afyon Kocatepe Üniversitesi, 2019) Öztürk, Caner; Güngör, Şükrü; Demir, Halit Furkan
    The aim of this study was to investigate the spermatological parameters of Aksaray Malakli shepherd dog after freezethawing semen with various additives. Study groups were set up as Tris-based extender (TBE)+trehalose (10mM),TBE+methionine (2mM) andcontrol. Sperm was collected by bulbus glandis massage (Digital Manipulation). Diluted semensamples were aspirated into 0,25 ml French straws and equilibrated at 5 ºC for 1,5 h. After equilibration, the straws werefrozen in liquid nitrogen vapor, plunged into liquid nitrogen for storage. For determination the sperm volume, grade semencollecting tubes were used and the results were recorded in milliliters. The sperm concentration was determined withhemocytometric method as mm3. Eosin-nigrosine staining method was used for live sperm percentages. The HOS-test wasperformed to determine the ratio of intact spermatozoa membrane. When the freeze-thawing results are statisticallyexamined with one-way analysis of variance, methionine group, motility (52,5%±2,7) and HOS test analysis (43,1%±2,9),(41,5%±3,1;33,2%±2,9) shown significant differences compared control (41,5%±3,1; 33,2%±2,9) group (p <0,05). The bestresult in the live sperm percentages was observed in methionine group but there was no statistically significant differencebetween the groups (p> 0.05).In the trehalose group, motility, HOS test and live sperm percentages results were 50.0%±3,9;60,6%±3,7;38,4±3,6%, respectively. As a result of the study, it was determined that methionine 2 mM added to trisbased diluent in Aksaray Malaklı shepherd dog sperm was beneficial on spermatological parameters. The cryoprotectiveeffect of methionine was found to be stronger than trehalose and control group.