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Öğe A novel matrix for hydrophobic interaction chromatography and its application in lysozyme adsorption(Polskie Towarzystwo Biochemiczne, 2014) Gedikli, Mehmet; Ceylan, Şeyda; Erzengin, Mahmut; Odabaşı, MehmetA novel 1-naphthylamine (NA) coupled poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(L)-histidine methyl ester) [NA-PHEMAH] supermacroporous monolithic hydrophobic cryogel was prepared via covalent coupling of NA to PHEMAH for adsorption of lysozyme from aqueous solution. Firstly, PHEMAH monolithic cryogel was prepared by radical cryocopolymerization of HEMA with MAH as a functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as a crosslinker directly in a plastic syringe, and then NA molecules were covalently attached to the imidazole rings of MAH groups of the polymeric structure. The prepared, NA-PHEMAH, supermacroporous monolithic hydrophobic cryogel was characterized by scanning electron microscopy (SEM). The effects of initial lysozyme concentration, pH, salt type, temperature and flow rate on the adsorption efficiency of monolithic hydrophobic cryogel were studied in a column system. The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 86.1 mg/g polymer at pH 8.0 with a flow rate of 1 mL/min. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the NA-PHEMAH monolithic hydrophobic cryogel without significant loss of the adsorption capacity.Öğe Application of cu2+-attached magnetite nanoparticles embedded supermacroporous monolithic composite cryogels for DNA adsorption(Hacettepe Üniversitesi, 2011) Ceylan, Şeyda; Kalburcu, Tülden; Gedikli, Mehmet; Odabaşı, MehmetKriyojeller ayırma biliminde yeni nesil sabit faz olarak değerlendirilmektedirler. Kriyojeller, altakım işlemlerinde protein, enzim, DNA, hücre organelleri ve virüsler gibi biyomoleküllerin ayrılması ve saflaştırılmasında etkin adsorbentler olarak kullanılmaktadır. Bu çalışmada, Cu2+-takılı magnetit (Fe3O4) nanopartikül (Cu2+-AMNP) gömülü süpermakrogözenekli monolitik kompozit kriyojellerin DNA adsorpsiyon performansı incelenmiştir. Öncelikle, 3-aminopropyltriethoxysilane (APTES) ile kaplanmış magnetit NP’ler silanizasyon reaksiyonu ile hazırlanmıştır. Daha sonra, silanlanmış magnetit NP’lere, amin grupları üzerinden Cu2+ iyonları takılmıştır. Bundan sonra, Cu2+-AMNP’ler DNA adsorpsiyon çalışmaları için süpermakrogözenekli monolitik kompozit kriyojellere gömülmüştür. Hazırlanan kriyojel taramalı elektron mikroskobu (SEM), elementel analiz, FTIR ve şişme testi ile karakterize edilmiştir. SEM analizleri kriyojellerin, donmuş su kristallerinin porojen etkisi ile oluşan birbirine bağlı 10-50 ?m boyutunda gözenekleri ile heterogözenekli bir yapıya sahip olduğunu göstermiştir. Maksimum DNA adsorpsiyon miktarı pH 8’de 19.97 mg/g polymer olarak bulunmuştur.Öğe Hepatotoxic microcystin removal using pumice embedded monolithic composite cryogel as an alternative water treatment method(Pergamon-Elsevier Science Ltd, 2016) Gürbüz, Fatma; Ceylan, Şeyda; Odabaşi, Mehmet; Codd, Geoffrey A.Microcystins are the most commonly encountered water-borne cyanotoxins which present short- and long-term risks to human health. Guidelines at international and national level, and legislation in some countries, have been introduced for the effective health risk management of these potent hepatotoxic, tumour-promoters. The stable cyclic structure of microcystins and their common production by cyanobacteria in water bodies at times of high total dissolved organic carbon content presents challenges to drinking water treatment facilities, with conventional, advanced and novel strategies under evaluation. Here, we have studied the removal of microcystins using three different forms of pumice particles (PPs), which are embedded into macroporous cryogel columns. Macroporous composite cryogel columns (MCCs) are a new generation of separation media designed to face this challenging task. Three different MCCs were prepared by adding plain PPs, Cu2+-attached PPs and Fe3+-attached PPs to reaction media before the cryogelation step. Column studies showed that MCCs could be successfully used as an alternative water treatment method for successful microcystin removal. (C) 2016 Elsevier Ltd. All rights reserved.Öğe Investigation of protein adsorption performance of Ni2+-attached diatomite particles embedded in composite monolithic cryogels(Wiley Periodicals, 2011) Ünlü, Nuri; Ceylan, Şeyda; Erzengin, Mahmut; Odabaşı, MehmetAs a low-cost natural adsorbent, diatomite (DA) (2 mu m) has several advantages including high surface area, chemical reactivity, hydrophilicity and lack of toxicity. In this study, the protein adsorption performance of supermacroporous composite cryogels embedded with Ni2+-attached DA particles (Ni2+-ADAPs) was investigated. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic composite cryogel column embedded with Ni2+-ADAPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as crosslinker directly in a plastic syringe for affinity purification of human serum albumin (H SA) both from aqueous solutions and human serum. The chemical composition and surface area of DA was determined by XRF and BET method, respectively. The characterization of composite cryogel was investigated by SEM. The effect of pH, and embedded Ni2+-ADAPs amount, initial HSA concentration, temperature and flow rate on adsorption were studied. The maximum amount of HSA adsorption from aqueous solution at pH 8.0 phosphate buffer was very high (485.15 mg/g DA). It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Ni2+-ADAPs in poly(2-hydroxyethyl methacrylate) composite cryogel without significant loss of adsorption capacity. The efficiency of albumin adsorption from human serum before and after albumin adsorption was also investigated with SDS-PAGE analyses.Öğe Phema mikroküre gömülü süper makrogözenekli kriyojel kompozit membranların üretilmesi ve protein adsorpsiyon kapasitelerinin incelenmesi(Aksaray Üniversitesi Fen Bilimleri Enstitüsü, 2011) Ceylan, Şeyda; Odabaşı, MehmetBu çalışmada, lizozimin etkin bir şekilde ayrılması için Afinite kromatografisi yöntemleri arasında, son yıllarda yoğun bir uygulama alanı bulan, İmmobilize metal afinite kromatografisi (İMAK) kullanılmıştır. Bu amaçla, yaklaşık 2 ?m çapında poli(2-hidroksietil metakrilat) (PHEMA) mikroküreler hazırlanmış, bu kürelere iminodiasetik asit (İDA) kovalent olarak bağlanmış, daha sonra da İDA üzerinden Cu2+ takılmıştır. Son olarak hazırlanan bu partiküller ile PHEMA partikül gömülü süpermakrogözenekli kriyojel kompozitler hazırlanmıştır. Bu amaçla, monomer olarak poli(2-hidroksietil metakrilat), çapraz bağlayıcı olarak N,N?-metilen-bis-akrilamid, başlatıcı/aktivatör olarak N,N,N´,N´-tetrametilen diamin (TEMED)/amonyum persülfat (APS) kullanılmış ve radikal kriyo-kopolimerizasyon yöntemiyle 5 mL?lik plastik tüplerde sentezlenmiştir. Hazırlanan Cu2+-takılı PHEMA mikroküreler gömülü süpermakrogözenekli kriyojeller yüzey alan tayini, şişme testleri, taramalı elektron mikroskobu (SEM), FTIR ile karakterize edilmiştir. Sulu çözeltiden lizozim adsorpsiyonuna pH?nın, tamponun derişimin, akış hızının, iyonik şiddetinin, ligand miktarının ve sıcaklığın etkisi incelenmiştir. Maksimum adsorpsiyon kapasitesi pH 9 tris-baz tamponunda, 5 mg/mL başlangıç lizozim derişiminde 107,14 mg/g polimer olarak belirlenmiştir. Ayrıca, Cu2+-takılı PHEMA mikroküreler gömülü süpermakrogözenekli kriyojeller üzerine enzimin defelarca adsorplanıp desorplanabildiği belirlenmiştir.
