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Öğe Effects of age and sex on some hematological and biochemical parameters in Hair goats(Urmia University - Faculty of Veterinary Medicine, 2022) Karaşahin, Tahir; Aksoy, Neşe Hayat; Dursun, Şükrü; Bulut, Gaye; Haydardedeoğlu, Ali Evren; Çamkerten, Güzin; Çamkerten, İlker; İlgün, RamazanThis study was performed to determine the blood reference values of hematological and biochemical parameters in Hair goats at different ages. For this aim, clinically healthy Hair goats (n = 180, ages of six month and 1-3 years male and female) were used to collect blood samples. In whole blood, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)and red cell distribution width (RDW) were evaluated. The levels of, albumin, total protein, globulin, glucose, total bilirubin, urea, creatinine, phosphorus, magnesium, calcium, aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatine kinase (CK), alanine aminotransferase (ALT) and iron levels were investigated in sera. Age and sex had no effect on the cell structures of blood. In the either sex (male or female), age had no effect. However, sex had a significant effect on the blood cell structures except for WBC, HGB, and HCT. Albumin, protein, calcium, AST, ALT, ALP and magnesium values were not different among groups. Both the age and sex of the goats had significant effects on blood levels of glucose, phosphorus, urea, cholesterol, creatinine, GGT, CK and total bilirubin. In conclusion, this was the first study reporting the hematologic and biochemical parameters in blood of the Hair goats. The results indicated that these parameters could show variations under physiological conditions due to age and gender.Öğe Efficiency of two different synchronization protocols in conception in simmental heifers(Afyon Kocatepe Üniversitesi, 2020) Bulut, Gaye; Taşdemir, UmutThis study aimed to reveal the effects of timed artificial insemination applied after the synchronization protocol known as day5 (D5) and the prostaglandin F2? (PGF2?) protocol applied in 11-day intervals (D11) on pregnancy rates in Simmental heifers. A total of 88 heifers were divided into two treatment groups. In the D5 group (n: 56), after gonadotrophin releasing hormone (GnRH) application, PGF2? injection was made on the 5th day and the second PGF2? injection was given one day later. The second GnRH was applied at the time of TAI and at the 44th hour of the second PGF2? application. In the D11 group (n: 32), double-dose PGF2? was given in 11-day intervals. GnRH was applied at the time of TAI and at the 81st hour following the second PGF2? injection. Pregnancy-associated glycoprotein visual test was carried out for determining to pregnancy on the 28th day. Additionally, for the purpose of determining embryonic deaths, the pregnancies were checked again by ultrasonography on the 63rd day following TAI. The 28th-day pregnancy rates in the synchronized heifers were 51.8% (29/56) in the D5 group and 43.8% (14/32) in the D11 group P>0.05. The 63rd-day pregnancy rates were 48.21% (27/56) in the D5 group and 40.62% (13/32) in the D11 group P>0.05. Consequently, although there was no statistically significant difference in terms of pregnancy rates between the D5 and D11 protocols, there was a numerical increase in the D5 protocol. Considering its short application time, D5 may be used for TAI in Simmental heifers.Öğe Evaluation of functional structures in the ovaries pre and post ovulation by doppler ultrasonography in bitches(Atatürk Üniversitesi Veteriner Fakültesi, 2021) Bulut, GayeIn this article, functional structures on the ovaries of bitches before and after ovulation are evaluated with Doppler ultrasonography and information concerning the interpretations of findings are transferred. Doppler effect is the first step of Doppler imaging. This effect is the shift in the frequency of the transmitted ultrasound. Spectral and/or color Doppler ultrasonography is the easiest and the most important diagnostic technique in assessing ovarian hemodynamic. The Doppler shift and the Doppler angle are used by the instrument for computing blood velocity. Spectral Doppler contains quantitative information. This information includes the peak systolic volume, end-diastolic volume, and time-averaged maximum velocities, Doppler indices (pulsatility index; PI; resistance index, RI). Doppler indices are used to assess vascular perfusion and computed using values PSV, EDV, and TAMV. The presence of blood flow in a color doppler image is shown as color-encoded and superimposed on a real-time B-mode image. Color Doppler imaging provides detailed monitoring of the local blood flow in ovarian follicles and corpus luteum. The discovery of Doppler ultrasonography that includes blood flow information has led to the formation of research issues in veterinary medicine. It is a developing technology in veterinary medicine. This technology is provided an understanding of hemodynamics and the relationship between blood flow and physiological-pathophysiological processes.Öğe Foliküler Kistli Holstein Irkı Düvelerde Ovsynch Protokolünün Gebelik Oranları Üzerine Etkisi(Erciyes Üniversitesi, 2023) Dursun, Şükrü; Köse, Mehmet; Kızıl, Seda Hamdi; Bulut, Gaye; Öztürk, Caner; Atlı, Mehmet; Karaşahin, TahirBu çalışmada, foliküler kistli Holstein ırkı düvelerde Ovsynch programının tedavideki etkinliğini belirlemek amaçlan- dı. Özel bir süt sığırı işletmesinde, iki veya üç tohumlamadan sonra gebe kalmayan, sık düzensiz, uzun veya sürekli östrüs belirtileri gösteren 2018 yılında 19 baş, 2022 yılında 17 baş, toplam 36 baş Holstein ırkı düve on gün arayla ikişer kez rektal palpasyon (RP) ve ultrasonografi (USG) ile tekniğine uygun olarak muayene edildi. İkinci muayeneden sonra, korpus luteum (CL) yokluğunda, 25 mm'den büyük çapta folikül taşıyan düveler (2018 yılında 13, 2022 yılında 12 olmak üzere toplam 25), foliküler kist grubuna dahil edilirken, kalan (2018’de 6, 2022 ‘de 5) 11 düve kontrol grubu olarak ayrıldı. Her iki gruptaki düvelere Ovsynch protokolü uygulandı. Düveler aynı ortamda aynı rasyon ile beslendi. Düvelerin gebelik muayeneleri tohumlamadan 45 gün sonra RP ve USG ile yapıldı. Elde edilen verilerin istatistiksel analizinde SPSS hazır paket programı kullanıldı. Gebelik oranı foliküler kist grubunda %88.0 (22/25), kontrol grubunda %72.73 (8/11) olarak tespit edildi. Grupların gebelik oranları arasında anlamlı fark yoktu (P?0.05). Sonuç olarak, Ovsynch protokolünün foliküler kisti olan düvelerde kullanılabileceği kanısına varıldı.Öğe Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats(Chartered Inst. of Building Services Engineers, 2022) Boztok Özgermen, Başak; Bulut, Gaye; Alparslan Pınarlı, Ferda; Gültekin, Salih Sinan; Özen, Doğukan; Yavuz, Orhan; Haydardedeoğlu, Ali EvrenThe potential protective effects of mesenchymal stem cells (MSCs) on some kidney diseases have been reported. However, the effect of the fetal kidney–derived (FKD)MSCs on doxorubicin-induced nephropathy has not been studied yet. This study aimed to treat rats with doxorubicin-induced kidney injuries by transplantation of –FKD-MSCs. Twenty-four Sprague-Dawley rats were divided into three groups as control, doxorubicin nephropathy (Sham), and doxorubicin + MSC treated group. Serum biochemistry analysis was performed at the beginning and the end of the study. Functional changes in kidneys were evaluated by scintigraphy. In the doxorubicin nephropathy group, histopathological findings such as mesangial cell proliferation, tubular cast, and glomerular hypertrophy were observed, whereas in the MSC group these findings were significantly reduced. CD133 and CD24 positive immunoreactions were the most severe and frequently observed in the MSC group. While positive staining was detected in the tubular epithelium, there was no immunostaining observed in the glomerulus. The results showed that both functional and histological improvements were achieved in the MSC group compared to the Sham group. In conclusion, transplantation of fetal kidney - derived MSCs into patients with renal damage is thought to contribute to the healing of the renal tissue.Öğe Koç spermasının kısa süreli saklanmasında katkı maddelerinin etkisi(İlker ÇAMKERTEN, 2021) Öztürk, Caner; Dursun, Şükrü; Bulut, Gaye; Karaşahin, TahirBu çalışmanın amacı, kısa süreli saklanan koç spermasının (24 saatlik aralıklarla) 72 saate kadar spermatolojik parametreler üzerine etkilerini araştırmaktı. Çalışmada % motilite, canlılık ve akrozom bütünlüğü verileri değerlendirildi. Çalışmada her koçtan (4 baş Merinos koçu) alınan dört ejakülat kullanıldı. Ejakülatlar pooling yapıldıktan sonra eşit hacimli yedi parçaya bölündü. Çalışma grupları, tris bazlı sulandırıcıya eklenen resveratrol (2 ve 4 mM), troloks (2 ve 4 mM), BSA (3 ve 6 mg/ml) ve kontrol olmak üzere oluşturuldu. Sulandırılan örnekler 72. saate kadar 4°C de muhafaza edildi. Spermatozoa motilitesi, canlılık ve akrozom bütünlüğü 0, 24, 48 ve 72. saatte değerlendirildi. Motilite muayenesi faz kontrast ataçmanlı mikroskopta 400X lük büyütmede, canlılık ve akrozom bütünlüğü ise floresan mikroskop ile değerlendirildi. Çalışmanın 72. saatinde, BSA (3 ve 6mM) grupları motilite (53,75±2,50%; 55,00±4,08%) ve akrozom bütünlüğü (53,70±3,39%; 57,10±4,68%) değerlendirmelerinde kontrol grubu (43,75±2,50%; 46,53±3,58) ile istatistiksel farklılık (p<0,05) gösterdi. Troloks (4mM) grubunda (67,22±3,71%), tüm gruplara kıyasla 72. saatte en yüksek canlılık sonucuna ulaşılmış ayrıca kontrol grubu (46,53±3,53) ile istatistiksel farklılık (p<0,05) belirlenmiştir. Çalışmanın sonucunda koç sperma sulandırıcısına eklenen BSA ve troloksun koç spermasının kısa süreli saklamasında faydalı olduğu ve spermatolojik parametreler üzerine koruyucu etki gösterdiği belirlenmiştir.Öğe Relationship between total antioxidant/oxidant status, and oxidative stress index and superovulation response in donor cows(Elsevier, 2021) Karaşahin, Tahir; Alkan, Hasan; Satılmış, Fatma; Dursun, Şükrü; Öztürk, Caner; Bulut, Gaye; Aksoy, Neşe Hayat; Tekindal, Mustafa Agah; Çağlayan, Tamer; Yeşilkaya, Ömer Faruk; Erdem, HüseyinThe aim of the present study was to determine the effects of serum total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) on counts of corpus luteum, transferable embryo, and embryo quality in cows treated with superovulation protocol. A total of 60 Simmental cows (postpartum 90 to 120 days) were used. The donors were treated with a 9 day-progesterone based estrus synchronization protocol. On the 7th day of the progesterone treatment, donors were treated with FSH twice daily as decreasing doses for 4 days. On day 9, PGF 2 injection was performed in the morning and progesterone source was removed in the evening. The cows were inseminated twice artificially on day 11 at 12 hours interval. On the day of artificial insemination, blood samples were collected to determine serum TAS, TOS and OSI concentrations. Uterine flushing was performed on day 7 after artificial insemination. Serum TAS, TOS and OSI concentrations of cows were 1.62 mu mol/L, 5.44 mu mol/L and 0.45 AU, respectively. There was a positive correlation between TAS concentration and the counts of transferable embryo, Code I and II quality embryo (P<0.05). TOS and OSI concentrations were negatively correlated with the counts of transferable embryo and Code I embryo, and positively correlated with unfertilized oocyte counts (P<0.05). As a result, it was concluded that TAS, TOS, and OSI affected the counts of transferable embryo, embryo quality, and unfertilized oocyte in donor cows.Öğe Serum cholesterol levels in hair goats of Aksaray Region(Agricultural Research Communication Centre, 2019) Karaşahin, Tahir; Aksoy, Neşe Hayat; Haydardedeoğlu, Ali Evren; Dursun, Şükrü; Bulut, Gaye; Çamkerten, Güzin; Çamkerten, İlker; İlgün, RamazanThis study was conducted to determine the levels of serum total cholesterol in Hair goats in different gender and age ranges. For this purpose, the blood samples from 120 Hair goats, young (<= 6 months) and adult (2-4 years), which were found to be clinically healthy, were taken from the vena jugularis. Triglyceride, total cholesterol, HDL and LDL cholesterol levels were measured in sera samples. The mean values in adult female goats, young female goats, adult male goats and young male goats were found. Triglyceride levels were found to be higher in male goats than in females. It was observed that the HDL values obtained from male kids were lower than the other ones. The LDL results obtained from the goats were close to each other and statistically insignificant. Thus, in this study, the data obtained is considered to contribute to the formation of reference values in the Hair goats.Öğe The effects of dry-off therapy on milk somatic cell count in saanen goats(Scientific Technical Research Council Turkey-TÜBİTAK, 2015) Baştan, Ayhan; Salar, Seçkin; Baki Acar, Duygu; Demirel, Murşide Ayşe; Cengiz, Mehmet; Darbaz, İsfendiyar; Bulut, GayeThe aim of this study was to determine the effectiveness of dry-off antibiotic therapy and teat sealant on somatic cell count (SCC) in Saanen goats. The goats were randomly divided into 3 groups. In Groups I (n = 50) and II (n = 50), the goats were treated with intramammary antibiotics and a combination of intramammary antibiotics and internal teat sealant at dry-off, respectively. The animals in Group III (n = 50) were designated as the control group. For the SCC analysis and bacteriological examination, milk samples (n = 900) were collected separately from each udder half during routine morning milking, prior to drying off, and at months 1, 2, 3, 4, and 5 of following lactation. The pretreatment prevalence of intramammary infection at dry-off was 4.7% (7/150) for halves. Isolated pathogens were coagulase-negative staphylococci (57.1%) and S. aureus (42.9%) from infected halves. Dry-off antibiotic and dry-off antibiotic with teat sealant therapy reduced milk SCC levels significantly (P < 0.01) when compared to the control group. On the other hand, the difference in SCC between dry-off antibiotic and dry-off antibiotic with teat sealant therapy groups was not statistically significant (P > 0.01). In conclusion, intramammary antibiotics administered alone and a combination of intramammary antibiotics and teat sealant reduced milk SCC levels significantly.Öğe The incidence of neospora caninum in dairy cows with abortion and infertility problems in Aksaray providence(Afyon Kocatepe Üniversitesi, 2021) Bulut, Gaye; Ün, Hikmet; Sanioğlu Gölen, Gökçenur; Camkerten, İlkerThe contribution of animal husbandry to the national economy cannot be ignored. The causes of infertility are various and complex. Economic losses are increasing in dairy cattle breeding due to infectious and non-infectious causes. The percentage of abortion due to infectious agents is not fully known, but infectious agents lie in about 90% of the cases whose etiology can be determined. Neospora caninum is considered to be one of the most important abortion factors of cattle. Although, this protozoa is always ignored. It causes abortion in cows. In this study, it was aimed to detect N. caninum from the blood serum obtained from 137 dairy cattle brought to Aksaray University Faculty of Veterinary Medicine between 2017-2019 and suffering from abortion and infertility problems. For serological diagnosis, ELISA (Enzyme-Linked ImmunoSorbent Assay) test was performed.As a result, N. caninum agent was detected in 37.22 % (51/137) of the samples that belong to cattle with abortion and infertility problems. It was concluded that economic loss due to infertility in dairy cattle and protozoa, which are one of the infection factors, should be given importance.