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  • Küçük Resim
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    A novel electrochemical biosensor based on palladium nanoparticles decorated on reduced graphene oxide-polyaminophenol matrix for the detection and discrimination of mitomycin C-DNA and acyclovir-DNA interaction
    (Elsevier B.V., 2023) Yanık, Suzan; Emre, Deniz; Alp, Meltem; Algı, Fatih; Yılmaz, Selehattin; Bilici, Ali; Özkan Arıksoysal, Dilşat
    Both the design of molecules that will interact specifically with DNA and the determination of the mechanism of action of this drug on DNA are important as they allow the control of gene expression. In particular, rapid and precise analysis of this type of interaction is a vital element for pharmaceutical studies. In the present study, a novel reduced graphene oxide/ palladium nanoparticles/ poly(2-amino-4-chlorophenol) (rGO/Pd@PACP) nanocomposite was synthesized by chemical process to modify pencil graphite electrode (PGE) surface. Here, the performance of the newly developed nanomaterial-based biosensor for drug-DNA interaction analysis has been demonstrated. For this purpose, it was determined whether this system, which was developed by selecting a drug molecule (Mitomycin C; MC) known to interact with DNA and a drug molecule (Acyclovir; ACY) that does not interact with DNA, performs a reliable/accurate analysis. Here, ACY was used as a negative control. Compared to bare PGE, the rGO/Pd@PACP nanomaterial modified sensor exhibited 17 times higher sensitivity performance in terms of guanine oxidation signal measured by differential pulse voltammetry (DPV). Moreover, the developed nanobiosensor system provided a highly specific determination between the anticancer drug MC and ACY by discrimination the interactiBoth the design of molecules that will interact specifically with DNA and the determination of the mechanism of action of this drug on DNA are important as they allow the control of gene expression. In particular, rapid and precise analysis of this type of interaction is a vital element for pharmaceutical studies. In the present study, a novel reduced graphene oxide/ palladium nanoparticles/ poly(2-amino-4-chlorophenol) (rGO/Pd@PACP) nanocomposite was synthesized by chemical process to modify pencil graphite electrode (PGE) surface. Here, the performance of the newly developed nanomaterial-based biosensor for drug-DNA interaction analysis has been demonstrated. For this purpose, it was determined whether this system, which was developed by selecting a drug molecule (Mitomycin C; MC) known to interact with DNA and a drug molecule (Acyclovir; ACY) that does not interact with DNA, performs a reliable/accurate analysis. Here, ACY was used as a negative control. Compared to bare PGE, the rGO/Pd@PACP nanomaterial modified sensor exhibited 17 times higher sensitivity performance in terms of guanine oxidation signal measured by differential pulse voltammetry (DPV). Moreover, the developed nanobiosensor system provided a highly specific determination between the anticancer drug MC and ACY by discrimination the interactions of these drugs with double-stranded DNA (dsDNA). ACY was also preferred in studies for the optimization of the new nanobiosensor developed. ACY was detected in a concentration as low as 0.0513 ?M (51.3 nM) (LOD), and limit of quantification (LOQ) was 0.1711 ?M with a linear range from 0.1 to 0.5 ?M. ons of these drugs with double-stranded DNA (dsDNA). ACY was also preferred in studies for the optimization of the new nanobiosensor developed. ACY was detected in a concentration as low as 0.0513 ?M (51.3 nM) (LOD), and limit of quantification (LOQ) was 0.1711 ?M with a linear range from 0.1 to 0.5 ?M.
  • Küçük Resim
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    Fabrication of PAMP/Au and GO/PAMP/Au nanosensors for electrochemical detection of paracetamol in pharmaceutical preparations
    (Springer, 2021) Bilici, Ali; Denizhan, Nuray; Emre, Deniz; Soylukan, Caner; Algı, Fatih; Yılmaz, Selehattin
    This paper reports on the modification of Pencil Graphite Electrode (PGE) surface with the poly(2-amino-4-methylphenol)/gold (PAMP/Au) and graphene oxide/poly(2-amino-4-methylphenol)/gold (GO/PAMP/Au) nanocomposites, in two steps. The first step is based on the one-pot preparation of composites by template-free chemical oxidation process. In the second step, composites are deposited at PGE surface by electro-oxidation process. Both nanocomposites and modified PGE surfaces are characterized by X-Ray Diffraction method (XRD), Scanning Electron Microscopy (SEM), EDAX, and CV analyses. The electrochemical performances of modified electrodes (abbreviated as PAMP/Au-PGE and GO/PAMP/Au-PGE) were investigated. The limit of detection values for PGE, PAMP/Au-PGE, and GO/PAMP/Au-PGE were found to be 2.74 × 10–6, 5.29 × 10–7, and 2.91 × 10–8 mol/dm3, respectively. The limit of quantification values were determined as 9.14 × 10–6, 1.76 × 10–6, and 9.69 × 10–8 mol/dm3 for PGE, PAMP/Au-PGE, and GO/PAMP/Au-PGE, respectively.
  • Küçük Resim
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    Graphene quantum dots-polyfluorene hybrid nanobiosensor for mitomycin C-DNA interaction sensing
    (Elsevier, 2024) Emre, Deniz; Denizhan, Nuray; Özkan Arıksoysal, Dilşat; Bilici, Ali; Sonkaya, Ömer; Algı, Fatih; Algı, Fatih; Yılmaz, Selehattin
    A novel graphene quantum dots (GQD) / polyfluorene (PF) nanocomposite was deposited on the disposable pencil tip graphite electrode (PGE) and proven to be an efficient nanosensor for analysis of the electrochemical interaction between the antitumor compound mitomycin C (MC) with double stranded DNA (ds-DNA). This modified electrode (GQD@PF-PGE) was prepared in four steps: hydrothermal, chemical oxidation, ultra-sonication and electro-oxidation processes. GQD, PF, GQD@PF and GQD@PF-PGE have been characterized by different analytical techniques such as SEM, TEM, XRD, FTIR, UV-Vis, EIS. Compared to bare PGE, GQD@PF modified PGE performed approximately 56 times more sensitive analysis when evaluating the guanine oxidation signals measured by DPV. CV and EIS measurements also showed that GQD@PF-PGE possesses a fast electron transfer as compared to bare electrode and exhibit a remarkable electrocatalytic activity towards both guanine and MC electrooxidation. Comprehensive optimization studies have also been carried out for the developed new nanobiosensor.