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Öğe Anticholinesterase, anti-?-glucosidase, antioxidant and antimicrobial effects of four Algerian propolis(Springer, 2022) Boulechfar, Safa; Zellagui, Amar; Bensouici, Chawki; Aşan Özüsağlam, Meltem; Tacer, Songül; Hanene, DjeghimThis study aimed to investigate the functional properties of four Algerian propolis collected from El-Menia, Oum el Bouaghi, El Harrouch and Collo regions. The total bioactive content, antioxidant, anti-enzymatic and antimicrobial effects of the four propolis methanolic extracts were evaluated using in vitro assays. The highest amount of total phenolic (561.99 ± 3.50 ?g GAE/mg E) and flavonoid content (76.98 ± 0.26 ?g QE/mg E) was found in propolis from El-Menia. Antioxidant tests (DPPH, ABTS, CUPRAC and ferric reducing power) revealed the strong scavenging and reducing abilities of the extracts. Anti-enzymatic assays against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and ?-glucosidase showed that all extracts possessed a potent inhibitory effect on ?-glucosidase better than acarbose and revealed the ability of extracts to inhibit BChE more effectively than AChE. However, the extract of propolis from El Menia was the best inhibitor of the three key enzymes with IC50 values of 11.40 ± 0.58 µg/mL 16.06 ± 0.85 µg/mL and 71.29 ± 2.73 µg/mL against ?-glucosidase, BChE and AChE, respectively. The antimicrobial assay indicated that all extracts were mainly active against Gram-positive bacteria and yeast and had bactericidal action in certain bacteria and bacteriostatic action in other ones. However, they had all fungistatic effect on C. albicans ATCC 10231. According to these results, Algerian propolis can be considered as a source of natural bioactive principles for dietary, pharmacological and medicinal applications.Öğe Chemical composition, antioxidant, and antimicrobial activities of two essential oils from Algerian propolis(De Gruyter Open Ltd, 2022) Boulechfar, Safia; Zellagui, Amar; Aşan Özüsağlam, Meltem; Bensouici, Chawki; Erenler, Ramazan; Yıldız, İlyas; Tacer, Songül; Boural, Hasna; Demirtaş, İbrahimThis study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of two essential oils (EOs) from Algerian propolis. The volatile constituents were analyzed by gas chromatography-mass spectrometry. Fifty components were identified from the oils. The major components were found to be: cedrol (17.0%), ?-eudesmol (7.7%), and ?-eudesmol (6.7%) in EO of propolis from Oum El Bouaghi (EOPO) whilst ?-pinene (56.1%), cis-verbenol (6.0%), and cyclohexene,3-acetoxy-4-(1-hydroxy-1-methylethyl)-1-methyl (4.4%) in EO of propolis from Batna (EOPB). The antioxidant properties of EOPO and EOPB were determined using 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) and cupric reducing antioxidant capacity (CUPRAC assays), respectively. Both EOs had more cupric ion reducing ability than scavenging ABTS•+ radicals. The antimicrobial potential of the two EOs against eight pathogens was assayed by the agar diffusion method and the mode of action was determined by microdilution assay. The results revealed that EOPB was bactericidal for all tested pathogenic bacteria and fungicidal for Candida albicans ATCC 10231, whereas, EOPO showed bacteriostatic effect against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC27853 and fungistatic effect against C. albicans ATCC 10231. Thus, the obtained results suggest the important use of propolis EOs as preservative agents.Öğe Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials(Pamukkale University, 2025) Chelgham, Abdelhakim; Saadi, Abdelkader; Merouane, Abdelaziz; Bensouici, Chawki; ÇçAKakmak, Yavuz SelimPhlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts._HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry.Öğe Phytochemical Characterization and Assessment of Antioxidant and Anti-Alzheimer Effects of Algerian Seseli Tortuosum(Wiley, 2025) Slimani, Abdelkader; Akkal, Wail; Benmekhbi, Ahmed Acheraf; Mosbah, Asma; Çakmak, Yavuz Selim; Lefahal, Mostefa; Bousetla, Ahlem; Bensouici, Chawki; Laouer, Hocine; Akkal, Salah; Nieto, GemaHPLC analysis, phytochemical screening, thin layer chromatography, polyphenols and flavonoid contents were conducted to determine the bioactive contents of the Algerian Seseli tortuosum plant. Antioxidant activity was tested using DPPH and ABTS scavenging assays, reducing power, phenanthroline and silver nanoparticle (SNP) assays. The anti-Alzheimer's activity of extracts was also performed invitro and in silico. Phytochemical analysis highlighted the richness ofthe extracts in terms of coumarins, terpenoids and quercetin. The quantitative determination of total polyphenols and flavonoids showed that the highest amounts occurredinthe dichloromethane (DCME) and methanolic (MeOH) extracts. The antioxidant activities indicated a moderate potential. Compared with galantamine, DCME had a significantly greater anti-Alzheimer effect on BChE(CI50=9.14 +/- 1.74 mu g/ml and 34.75 +/- 1.99 mu g/ml respectively). An in silicostudy of anti-Alzheimer activity revealed a significant effect of quercetin(-30,13KJ/mol). Conclusion: This study demonstrated the richness of the phytochemical components of seseli tortuosum,which are responsible for several biological properties, mainly their anti-Alzheimer potential.
