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Öğe Comparison of bovine serum albumin adsorption capacities of alpha-chitin isolated from an insect and beta-chitin from cuttlebone(Elsevier, 2016) Kaya, Murat; Sargın, İdris; Aylanç, Volkan; Tomruk, Muhammed Nebi; Gevrek, Seda; Karatoprak, Işıl; Çolak, Nazlıcan; Sak, Yaşar Gül; Bulut, EsraBovine serum albumin is the key protein in blood. Chitin is a widely used biocompatible polymer in bioengineering and it is mainly found as two allomorphs (alpha, beta). In this study the interactions of alpha-chitin (from the insect Omophlus sp.) and beta-chitin (from the cuttlebone of Sepia sp.) with BSA were studied. Chitin isolates were examined by SEM, XRD, BET, TGA and FFIR. beta-Chitin exhibited a far higher affinity for BSA than alpha-chitin, indicating alpha-chitin can be used in applications where surface-protein interactions should be limited, and beta-chitin can be used in chitin-based materials on which protein adsorption is desired.Öğe In vitro studies on different extracts of fenugreek (Trigonella spruneriana BOISS.): Phytochemical profile, antioxidant activity, and enzyme inhibition potential(Blackwell Publishing Ltd, 2020) Aylanç, Volkan; Eskin, Bülent; Zengin, Gökhan; Dursun, MehmetThe side effects of synthetic antioxidants make it necessary to find a natural alternative. Therefore, the current study investigates the potential of T. spruneriana as a new alternative in terms of natural bioactive components. In this context, antioxidant activity, enzyme inhibition, and phenolic compounds of different extracts including ethanol, methanol, ethyl acetate, and aqueous were identified. The results show that the ethyl acetate (113.59 ± 2.73 mg GAE/g) has the highest phenolic content, but ethanol extract has the highest scavenging activity for DPPH and TAC. The ethanol extract showed stronger inhibition on cholinesterase and ?-amylase compared to other extracts. Besides, 12 bioactive compounds were characterized in T. spruneriana extracts by HPLC-DAD. Our findings support that T. spruneriana could be considered as a new source of active phytochemicals, as well as provide remarkable data on biological activities of some main enzymes playing role in the healing of hyperpigmentation, Alzheimer, and diabetes. Practical applications: This study reports the total content, types and amounts of bioactive compounds and potential beneficial bioactivities of the different extracts of T. spruneriana. Trigonella is abundant in nature and spread over a wide geographical area, and is used in making cheese, pastries, spices, and sausages in different countries, as well as for antidiabetic purposes. Trigonella leaves are a good source of bioactive compounds that contain compounds like quercetin, catechin, cinnamic acid, and coumaric acid, along with it have also a high content of soluble fibers and is suggested for body weight control. Apart from being the first study conducted to point out the potential of T. spruneriana as being a natural food additive, this study also demonstrated its medicinal importance by revealing the anti-hyperpigmentation, antidiabetic, neuroprotective, and antioxidant properties of T. spruneriana.Öğe Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae)(İstanbul Üniversitesi, 2020) Pamukçu, Damla; Aylanç, Volkan; Eskin, Bülent; Zengin, Gökhan; Dursun, Mehmet; Çakmak. Yusuf SelimBackground and Aims: Most of the Medicago species are considered good quality forage crops due to their rich protein content. This study reports antioxidant properties, total phenolic and flavonoid content, enzyme (acetylcholinesterase (AChE), tyrosinase, alpha-amylase and alpha-glucosidase) inhibition activities of methanol (MeOH), ethyl acetate (EA) and, aqueous (AQ) extracts of Medicago murex (M. murex). Methods: The antioxidant activity of the different extracts of M. murex were evaluated using FRAP and CUPRAC assays. The contents of total phenolic and flavonoid were determined by the Folin-Ciocalteu and the Aluminium chloride (AlCl3) cotorimetric methods. Also, the enzyme inhibition activities were shown spectrophotometrically for AChE, alpha-amylase, alpha-glucosidase and tyrosinase enzymes. Results: Total phenolic and flavonoid contents of different extracts were determined between 83.70-163.93 mg GAE/g extract and 24.48-26.05 mu g QE/g extract, respectively. AQ extracts were the most active extracts in FRAP while being the best inhibitor of the AChE enzyme. On the other hand, EA extracts were the most active extracts in CUPRAC methods and the best inhibitors for other enzymes. Conclusion: Considering the findings, M. murex appears to bean important source of natural antioxidants and enzyme inhibitors, and it provides important baseline data for the food and pharmaceutical industries.Öğe Natural ?-chitin-protein complex film obtained from waste razor shells for transdermal capsaicin carrier(Elsevier B.V., 2020) Aylanç, Volkan; Ertosun, Şeymanur; Akyüz, Lalehan; Koç Bilican, Behlül; Gökdağ, Semih; Bilican, İsmail; Çakmak, Yavuz Selim; Akyüz Yılmaz, Bahar; Kaya, MuratIn the literature, the produced ?-chitin samples are in powder or flake forms but there is no natural ?-chitin based film. Also, the commercially available transdermal patches are produced from synthetic polymers. In this regard, we produced natural ?-chitin-protein complex (CPC) film from the waste shells of Ensis spp. The obtained natural film was characterized by FTIR, TGA and SEM. Additionally, swelling, thickness, contact angle and antioxidant tests were done to learn more about the films. After production and characterization of the film, capsaicin, which is commonly used for pain relief was loaded into the film. The loading capacity was recorded as 5.79%. The kinetic models were studied in three different pH, then the results were fitted with Higuchi model with high correlation at pH 7.4. After considering all the obtained results, the capsaicin loaded CPC film may be an alternative candidate for transdermal patch instead of the synthetic ones.
