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    Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on ITS1-5.8S rRNA from Van Province, Turkey
    (University of Agriculture, 2025) Yılmaz, Ali Bilgin; Azizoğlu, Erkan; Adizel, Özdemir; Göz, Yaşar; Çelik, Burcak Aslan; Çelik, Özgür Yaşar; Ayan, Özge Oktay; Akhmetzhanova, Aizhan; Ayan, Adnan; Yasul, Muhammed; Myrzhiyeva, Assem; Uslu, Uğur
    This study aimed to calculate the occurence of Moniezia species in cattle, sheep, and goats in Van province and to identify these species using morphological and molecular methods (ITS1-5.8S rRNA gene region analysis). Additionally, the study aimed to identify the genetic differences between Moniezia expansa and Moniezia benedeni. During the summer of 2022, intestinal contents were collected from 150 ruminants (50 cattle, 50 sheep, and 50 goats) slaughtered in slaughterhouses in Van province. The parasites were examined using Aceto-Carmine staining, and species identification was based on interproglottidal glands. Examination of the intestinal contents revealed that 2 out of 50 cattle (4%), 14 out of 50 sheep (28%), and 9 out of 50 goats (18%) were infected with Moniezia. Morphological and molecular analyses showed that the cattle samples were identified as Moniezia benedeni, goats samples as Moniezia expansa and those from sheep as 11 Moniezia expansa and 3 Moniezia benedeni. Following DNA extraction, the ITS1-5.8S rRNA gene region was amplified using PCR and subjected to sequence analysis. The relationship between species was examined by phylogenetic tree. This study confirms the prevalence of Moniezia spp. in Van/Türkiye by using the ITS1-5.8S rRNA gene.
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    Serum thiol disulphide levels among sheep with sarcoptic mange
    (Veteriner Fakultesi Dergisi, 2019) Çamkerten, İlker; Çamkerten, Güzin; Erdo?an, Hasan; Ayan, Adnan; Erdoğan, Songül; Ural, Kerem
    Sarcoptic mange, a notable parasitic disease, causes dermatological alterations among ruminants. Thiol-disulphite hemostasis is a novel oxidative stress parameter. The aim of this study was to evaluate dynamic thiol/disulfide homeostasis in sheep with sarcoptic mange. Total of thirty-six sheep (n=15 female, n=21 male) with sarcoptic mange (Group I), and twelve healthy sheep (Group II) were used in the study. A novel method was used to determine the thiol disulphide parameters. Native thiol, total thiol and Disulphide values were statistically lower in Group I. Disulphide/native thiol, Disulphide/total thiol, and Native thiol/ total thiol proportions had no statistical differences in groups. Sarcoptic mange was probably affected by the thiol Disulphide hemostasis in infected sheep. Thus, the data obtained in this study might form base for further studies to include antioxidant molecules in the treatment protocols.