Yazar "Altun, Nurver" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Characterization of chitin and description of its antimicrobial properties obtained from Cydalima perspectalis adults
    (Springer Science and Business Media Deutschland GmbH, 2024) Kılcı, Leyla; Altun, Nurver; Karaoğlu, Şengül Alpay; Karaduman Yeşildal, Tuğçe
    Chitin is the most abundant biopolymer group after cellulose and forms the exoskeleton of arthropods, the largest animal group. The morphology of chitin differs between and within species. In this study, we determined the physicochemical and biological activity of chitin samples obtained from different body parts of the boxwood moth Cydalima perspectalis for its application as a biotechnological material. The collected chitin samples were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and elemental analysis. FTIR confirms that the isolation of chitin is successful. The SEM results showed that the surface morphology of the obtained chitins was both fibrous and porous and had a rough surface. As a result of the elemental analysis, the %N values of chitins were calculated as 6.60 on average, and the values were shown to be close to each other. We also investigated the biocompatibility and antimicrobial properties of these chitin samples. We used L929 (mouse fibroblast) cells to perform indirect cytotoxicity experiments and investigated their viability by performing the MTT assay. Our findings showed that the samples had no cytotoxic effect on the L929 cells at 24, 48, and 72 h. The cytotoxic study showed that Escherichia coli, Bacillus subtilis, Staphylococcus aureus bacteria, and Candida albican fungi adhered to chitin surfaces regarding biofilm production. The chitin contents were determined as 21.02% for the head, 5.74% for the body, 32.22% for the wing, 33.53% for the legs, and 2.65% for the pupal shell. Chitin is a material with high potential for use in various fields. Our findings suggested that Cydalima perspectalis can be used as an alternative source of chitin in biomedical application.
  • Küçük Resim
    Öğe
    Isolation, characterization, antimicrobial and cytotoxic properties of Cydalima perspectalis silk proteins
    (Elsevier B.V., 2025) Kılcı, Leyla; Altun, Nurver; Bozdeveci, Arif; Karaduman Yeşildal, Tuğçe
    Natural silk produced by silkworms and some arthropod spiders stands out as a traditional protein polymer. Fibroin and sericin, which are silk proteins obtained from cocoons, have been used in the medical field for centuries. This study is the first to evaluate the physicochemical properties and biological activities of these proteins, which were isolated from the boxwood moth (Cydalima perspectalis), as a biotechnological material. This followed this separation of the fibroin and sericin proteins obtained from the cocoons using a high-temperature and high-pressure method. The morphological, thermal, structural, and elemental properties of the obtained fibroin and sericin were analyzed using SEM, FTIR, TGA, XRD as well as elemental analysis. These studies were followed by biocompatibility and antimicrobial studies of the proteins. L929 (mouse fibroblast) cells were used in cytotoxicity assays, with cell viability evaluated using the MTT method. The study showed that the serine had no cytotoxic effect on NIH3T3 cells, whereas fibroin was cytotoxic for L929 cells. Fibroin surfaces exhibited antimicrobial activity by supporting the adhesion of, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and the fungus Candida albicans. This study demonstrates that silk proteins derived from other organisms can be used as alternative biomedical materials.