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  • Küçük Resim
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    A novel electrochemical biosensor based on palladium nanoparticles decorated on reduced graphene oxide-polyaminophenol matrix for the detection and discrimination of mitomycin C-DNA and acyclovir-DNA interaction
    (Elsevier B.V., 2023) Yanık, Suzan; Emre, Deniz; Alp, Meltem; Algı, Fatih; Yılmaz, Selehattin; Bilici, Ali; Özkan Arıksoysal, Dilşat
    Both the design of molecules that will interact specifically with DNA and the determination of the mechanism of action of this drug on DNA are important as they allow the control of gene expression. In particular, rapid and precise analysis of this type of interaction is a vital element for pharmaceutical studies. In the present study, a novel reduced graphene oxide/ palladium nanoparticles/ poly(2-amino-4-chlorophenol) (rGO/Pd@PACP) nanocomposite was synthesized by chemical process to modify pencil graphite electrode (PGE) surface. Here, the performance of the newly developed nanomaterial-based biosensor for drug-DNA interaction analysis has been demonstrated. For this purpose, it was determined whether this system, which was developed by selecting a drug molecule (Mitomycin C; MC) known to interact with DNA and a drug molecule (Acyclovir; ACY) that does not interact with DNA, performs a reliable/accurate analysis. Here, ACY was used as a negative control. Compared to bare PGE, the rGO/Pd@PACP nanomaterial modified sensor exhibited 17 times higher sensitivity performance in terms of guanine oxidation signal measured by differential pulse voltammetry (DPV). Moreover, the developed nanobiosensor system provided a highly specific determination between the anticancer drug MC and ACY by discrimination the interactiBoth the design of molecules that will interact specifically with DNA and the determination of the mechanism of action of this drug on DNA are important as they allow the control of gene expression. In particular, rapid and precise analysis of this type of interaction is a vital element for pharmaceutical studies. In the present study, a novel reduced graphene oxide/ palladium nanoparticles/ poly(2-amino-4-chlorophenol) (rGO/Pd@PACP) nanocomposite was synthesized by chemical process to modify pencil graphite electrode (PGE) surface. Here, the performance of the newly developed nanomaterial-based biosensor for drug-DNA interaction analysis has been demonstrated. For this purpose, it was determined whether this system, which was developed by selecting a drug molecule (Mitomycin C; MC) known to interact with DNA and a drug molecule (Acyclovir; ACY) that does not interact with DNA, performs a reliable/accurate analysis. Here, ACY was used as a negative control. Compared to bare PGE, the rGO/Pd@PACP nanomaterial modified sensor exhibited 17 times higher sensitivity performance in terms of guanine oxidation signal measured by differential pulse voltammetry (DPV). Moreover, the developed nanobiosensor system provided a highly specific determination between the anticancer drug MC and ACY by discrimination the interactions of these drugs with double-stranded DNA (dsDNA). ACY was also preferred in studies for the optimization of the new nanobiosensor developed. ACY was detected in a concentration as low as 0.0513 ?M (51.3 nM) (LOD), and limit of quantification (LOQ) was 0.1711 ?M with a linear range from 0.1 to 0.5 ?M. ons of these drugs with double-stranded DNA (dsDNA). ACY was also preferred in studies for the optimization of the new nanobiosensor developed. ACY was detected in a concentration as low as 0.0513 ?M (51.3 nM) (LOD), and limit of quantification (LOQ) was 0.1711 ?M with a linear range from 0.1 to 0.5 ?M.
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    Eu(III)-DO3A and BODIPY dyad as a chemosensor for anthrax biomarker
    (Wiley, 2021) Alp, Meltem; Algı, Melek Pamuk; Algı, Fatih
    The sensitive and selective determination of Bacillus anthracis spores before the infection is vital for human health and safety. Dipicolinic acid (DPA) is an excellent biomarker due to its presence in the nucleus of bacterial spores at high concentrations (up to 1 M, about 15% dry weight). In the present work, a new molecular chemosensor 1, based on europium(III)-DO3A and BODIPY dyad, is developed to detect DPA in phosphate-buffered saline (PBS) buffered solution and tap water samples. Also, 1 can be used as a ratiometric optical chemosensor to track DPA.
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    Tb(III)-DO3A and BODIPY dyad as multimode responsive hypochlorite probe
    (Elsevier, 2022) Alp, Meltem; Algı, Melek Pamuk; Algı, Fatih
    A multimode responsive hypochlorite probe 1, based on Terbium (III)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (Tb(III)-DO3A) and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), is described. We have shown that probe 1 can detect ClO- by absorption, fluorescence, and phosphorescence simultaneously. The multimode response makes probe 1 a versatile ClO- probe for practical applications. We have found that probe 1 can be used in naked-eye colorimetric and fluorogenic detection of NaOCl in solution. Also, we have constructed a colorimetric test paper for visual sensing of NaOCl. Furthermore, fluorescence imaging studies indicated that probe 1 was a versatile tool for in vitro imaging of NaOCl in living cells. Thus, to the best of our knowledge, probe 1 represents one of the rare examples of multimode responsive ClO- probes.
  • Küçük Resim
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    Terbiyum kopleksi bağlı indirgenmiş grafen oksit esaslı nano malzeme geliştirilmesi: Tasarım ve sentez
    (Aksaray Üniversitesi Fen Bilimleri Enstitüsü, 2019) Alp, Meltem; Algı, Fatih
    Bu tez çalışmasında terbiyum (III) kompleksi içeren grafen esaslı bir nano malzemenin tasarımı ve geliştirilmesi üzerinde durulmuştur. Bu amaçla öncelikle grafitten grafen oksit ve indirgenmiş grafen oksit uygun yöntemler üzerinden elde edilmiştir. Tasarlanan terbiyum (III) kompleksinin sentezinin ardından indirgenmiş grafen oksit ile birleştirilerek hedeflenen nano malzeme elde edilmiştir. Bu yeni malzemenin karakterizasyonu FT-IR, SEM, TEM ve Zeta potansiyeli yöntemleri kullanılarak yapılmıştır. Son aşamada ise nano malzemenin biyolojik olarak önem arz eden bazı iyon ve/ya moleküllerin tayin ve tespitinde lüminesans sensör/belirteç olarak kullanılıp kullanılamayacağı test edilmiştir. Elde edilen sonuçlar malzemenin en önemli reaktif oksijen türlerinden biri olan hidroksil radikalinin tayin ve tespitinde kullanılabileceğini göstermektedir.