Yazar "Akyuz, Bahar" seçeneğine göre listele

Listeleniyor 1 - 1 / 1
  • Küçük Resim
    Öğe
    Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies
    (ELSEVIER SCIENCE BV, 2016) Kaya, Murat; Mulercikas, Povilas; Sargın, İdris; Kazlauskaite, Sonata; Baublys, Vykintas; Akyuz, Bahar; Bulut, Esra; Tubelyte, Vaida
    Physicochemical characterization of new chitin isolates can provide valuable insights into designing of biomimetic materials. Chitin isolates with a definite three-dimensional (3D) structure can exhibit characteristics that distinguish them from other chitin specimens that are in form of powder or flakes without a definite and uniform shape. Herein, 3D chitin rings were produced from body segments of a diplopod (Archispirostreptus gigas) inhabiting tropical regions. This organism is cultured easily and can reach 38 cm in length, which makes it a suitable source for isolation of chitin. The chitin rings were characterized via TGA, FT-IR, SEM and XRD analyses. Enzymatic digestion test with chitinase demonstrated that chitin isolates had high purity (digestion rate: 97.4%). The source organism had high chitin content; 21.02 +/- 2.23% on dry weight. Interactions of the chitin rings with bovine serum albumin (BSA) protein were studied under different conditions (pH: 4.0-8.0, chitin amount: 6-14 mg, contact time: 30-360 min, protein concentration: 0.2-1 mg/mL). The highest BSA adsorption was observed at pH 5.0 at 20 degrees C. The adsorption equilibrium data exhibited a better fit to Langmuir adsorption and the pseudo-first order kinetic models. The findings presented here can be useful for further studies aiming to develop biocompatible and nontoxic biomaterials.