Yazar "Akyol, Numan" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    Investigation of bull effect on in vitro embryo production
    (Kafkas Üniversitesi, 2014) Akyol, Numan; Kızıl, Sedat Hamdi; Satılmış, Muharrem; Karaşahin, Tahir
    The aim of the study was to show whether there were some differences among 9 Holstein bulls and within their replications on their ability of in vitro fertilization for in vitro embryo production as cleavage and coming into the blastocyst stage. Semen collected and frozen from nine Holstein bulls with satisfactory in vivo fertilization capabilities for artificial insemination was used for in vitro fertilization. Direct washing method by Brackett and Oliphant medium and 5 or 6 h incubation period were used for in vitro fertilization. Charles Rosenkrans medium was used for in vitro embryo culture. An atmosphere with a higher than 95% relative humidity, 39°C, 5% CO2 and was used for all in vitro embryo production processes. Totally 2519 A and B quality oocytes were treated for in vitro embryo production. As a result statistically significant (P0.05) between the replications for each bull as cleavage and coming into blastocyst stage. The results showed varied capabilities of bulls for in vitro fertilization and embryo production and male factor can affect success of in vitro embryo production
  • Küçük Resim
    Öğe
    Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos
    (TÜBİTAK, 2014) Karaşahin, Tahir; Akyol, Numan; Satılmış, Muharrem; Bucak, Mustafa Numan; Çoyan, Kenan
    The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Sevenday-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively. The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%.
  • Küçük Resim
    Öğe
    Yerli kara düvelerde farklı süperovulasyon protokolleri ile elde edilen cevapların araştırılması
    (T.C. Tarım ve Orman Bakanlığı Uluslararası Hayvancılık Araştırma ve Eğitim Merkezi Müdürlüğü, 2017) Satılmış, Muharrem; Akyol, Numan; Kızıl, Sedat Hamdi; Karaşahin, Tahir
    Sunulan çalışmanın amacı, süperovulasyon uygulamalarına yanıtı düşük olan Yerli Kara düvelerde, farklı FSH protokolleri ile oluşturulan uygulamaların süperovulasyon etkinliğine yönelik sonuçlarının incelenmesidir. Çalışmada kullanılan 24 baş Yerli Kara düve, rastgele seçilerek dört gruba ayrılmış ve dört farklı süperovulasyon protokolü gerçekleştirilmiştir. Çalışma sonunda, tespit edilen korpus luteum (CL) sayıları üzerine farklı superovulasyon protokollerinin etkinliği görülmemiştir (P>0.05). Eksojen progesteron uygulanmamış grupta (G1), fertilize olmamış oosit (UFO) oranları diğerlerine göre daha yüksek (%43.8) bulunmuştur (P<0.01). Dördüncü grupta (G4) ise diğer gruplara göre daha yüksek oranda embriyo (%72.7) elde edilmiştir (P<0.001). Sonuç olarak Yerli Kara düvelerde uygulanan farklı süperovulasyon protokollerine rağmen yeterli düzeyde cevap gelişmediği gözlenmiştir.