Yazar "Akkurt, Mustafa Yenal" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    Genomewide association of male reproductive traits in Aksaray Malakli dogs
    (Wiley, 2018) İnanç, Muhammed Enes; Tekin, Koray; Akkurt, Mustafa Yenal; Olgaç, Kemal Tuna; Yılmaz, Burak; Çil, Beste; Kızılaslan, Mehmet; Taşdemir, Umut; Tuncer, Purhan Barbaros; Büyükleblebici, Serhat; Uysal, Ongun; Çınar Kul, Bengi
    Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) genotype of the investigated characteristics and reproductive loci. A total of 46 male dogs are divided into three age groups (I <= 3 years, n = 19; II 4-6 years; n = 19, and III >= 7 years, n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The Affymetrix Canine 127 K SNP genotyping array v2 (Affymetrix Inc., California, USA) was used for SNP genotyping. In the primary results, the scrotal circumference was found to be higher in group II compared to other groups (p < 0.05) and the lowest total abnormal spermatozoa rate was found in group I (p < 0.05). The normal spermatozoa rate was found to be significantly above the threshold in relation to the SNP in chromosome 17. In conclusion, this study represents an exciting first step towards SNP association with dog semen spermatological parameters. Future studies might be undertaken to evaluate this SNP region for gene-knockout and expression analysis and for fine mapping to validate and/or discover the exact position of the effect region.
  • Küçük Resim
    Öğe
    Investigation of ivermectin susceptibility in Kangal and Akbaş Dogs via MDR1 gene mutation
    (Harran Üniversitesi, 2024) Baydan, Emine; Çınar Kul, Bengi; Akkurt, Mustafa Yenal; Yıldırım, Ebru; Arslanbaş, Emre; Gönül Aydıni Farah
    This research aimed to investigate the sensitivity to the drug (ivermectin) in Kangal and Akbaş breed dogs, which are dog breeds native to Turkey, via the MDR1 gene mutation. For the research, blood, hair and intraoral swap samples were taken from 30 Kangal and 20 Akbaş breed dogs (male-female mixed, adult) with ethical permission and approval forms. Kangal dog samples were taken from the dogs bred in the farms in Sivas center and Kangal village, and Akbaş dog samples were taken from the dogs bred in the farms in the Sivrihisar center, Ankara and Eskişehir regions. The samples taken were evaluated based on polymerase chain reaction (PCR) from the wool and subsequent two-way dideoxy chain termination reaction in the presence of 4 base farm deletions (c.296-299delAGAT) in the 4th exon of the ABCB1 gene that encodes the P-glycoprotein (P-gp) drug transporter protein. According to the obtained DNA sequence results, the deletion of “AGAT” was not determined in any of the individuals screened. The results of this study, which is preliminary research, showed that Kangal and Akbaş breed dogs are safe in terms of sensitivity to drugs that are set to be absorbed and excreted by the P-gp pump, especially ivermectin. However, it would be useful to repeat the analysis of both breeds with more examples.