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  • Küçük Resim
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    Development of a simple and rapid DNA extraction method for aspergillus Flavus-A
    (Sciendo, 2024) Sanio?lu Gölen, Gökçenur; Akar, Kadir
    Aspergillus species are known to be very important in human and domestic animal health. Aspergillus species commonly cause severe systemic and skin infections, as well as allergic lung diseases. With the development of PCR techniques, these methods are used to identify and diagnose fungi. DNA extraction from Aspergillus species is difficult because the fungal cell wall structure is very durable and complex. Fungal DNA extraction methods containing proteinase K and liquid nitrogen are widely used to break down the cell wall. However, these methods cause DNA loss during the extraction in Aspergillus species. In this study, on the contrary, the commonly used DNA extraction by means of ammonium hydroxide, which is generally used to break down chitin in DNA extraction of ticks and plants, is used. The efficiency of the cell wall lysis method from A. flavus with ammonium hydroxide was compared with methods containing proteinase K and liquid nitrogen. For this purpose, DNA extraction of A. flavus was tried using three different methods. As a result, the cell wall of A. flavus was lysed using ammonium hydroxide in this study. The obtained DNA's quality, concentration, and PCR performance were sufficient. This method has been evaluated as a faster, more straightforward, and more economical alternative.
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    Öğe
    Investigation of the Correlation Between ELISA and Serum Amyloid A in the Diagnosis of Bordetella bronchiseptica in Dogs
    (John Wiley and Sons Inc, 2025) Sanioğlu Gölen, Gökçenur; Akar, Kadir; Ekin, İsmail Hakkı
    Background: Bordetella bronchiseptica is an essential bacterial pathogen characterized by chronic respiratory disease in dogs known as Kennel cough. The presence of causative antibodies in animals can also be detected by lipopolysaccharide antigen-based enzyme linked immunosorbent assay (ELISA). In recent years, it has been determined that there is a significant relationship between acute phase proteins and diseases, and disease follow-up can be done within the framework of this relationship. Methods: In this study, blood sera from 150 dogs in an animal shelter in Van province were evaluated for B. bronchiseptica by the homemade ELISA method, and their correlations with serum amyloid A (SAA) were investigated. Blood serum samples were analysed for antibodies against B. bronchiseptica using a homemade ELISA method. Positive animals were also molecularly confirmed using nasal swabs by PCR. A commercial ELISA kit determined SAA levels in blood sera. Results: Eighteen (12%) of the analysed blood serum samples were found positive by the homemade ELISA method. SAA concentrations in the positive blood sera were elevated from 12.7 to ≤38.98 mg/L. SAA concentrations in blood sera serologically positive for B. bronchiseptica were statistically significant. Conclusions: In this study, in which the relationship between SAA concentration and B. bronchiseptica was investigated for the first time in Turkey, it was concluded that SAA concentration analysis may help diagnose and monitor the disease. In addition, the presence and prevalence of this critical and zoonotic agent causing chronic respiratory tract disease in dogs in Van province was revealed for the first time in this study.
  • Küçük Resim
    Öğe
    Investigation of the presence and prevalence of listeriosis in clinical samples in Van and its region
    (Harran Üniversitesi, 2024) Akar, Kadir; Sanioğlu Gölen, Gökçenur; Babaoğlu, Ali Rıza; Ekin, İbrahim Hakkı
    Listeriosis is an infection that causes abortion in humans and various animals worldwide. The causative agent is spread by livestock faeces, especially ruminants, and has a zoonotic character, transmitted by ingesting contaminated silage and feed. In this study, it was aimed to investigate the presence of Listeria spp. in 120 samples (79 abortion material, 41 brain material) of sheep. For this purpose, the polymerase chain reaction (PCR) method was performed using specific oligonucleotide pairs for Listeria spp. all of the abortion materials from Van province and its districts were found to be negative. However, a total of 2 (4.87%) samples, one each from the Erciş and Gevaş districts, from sheep with clinical nervous symptoms were found positive. As a result, it was determined that Listeria spp. was sporadic in Van province. It was concluded that this situation may be due to the low use of silage in ovine breeding in Van province. It was thought that periodical studies should be carried out to determine the course of the disease in the region.