METHOD VALIDATION AND PREVALENCE OF L. Monocytogenes CONTAMINATION IN GROUND BEEF WITH VIDAS: AS AN ALTERNATIVE METHOD COMPARED WITH ISO 11290

Abstract

L. monocytogenes, which is recognized among the leading foodborne pathogen has been identified as the causative agent of listeriosis, which causes important symptoms such as abortion, septicemia, and meningitis. In the first stage of our study, the effectiveness of VIDAS LMO2 and ISO 11290:1 methods, which are frequently used today in the diagnosis of this pathogen, were compared in artificially contaminated samples. In the second stage, the presence of L. monocytogenes in ground beef samples sold in the market was revealed by both methods. According to the data achieved in the study, the relative sensitivity, specificity, accuracy, false positive, and false negative of the VIDAS LMO2 method against ISO 11290-1:2017 was found 99.236%, 73,684%, 90%, 0.263%, and 0.007% in ground beef, respectively. Although no significant difference was detected between the two methods, VIDAS LMO2 was found to be superior in terms of analysis time. Since it is known that rapid and reliable diagnosis is very important in food poisoning, the VIDAS method can be preferred more desirable due to this superiority.