Acet, ÖmürÖnal, BurcuSanz, RaulSanz-Perez, Eloy S.Erdönmez, DemetOdabaşı, Mehmet13.07.20192019-07-1613.07.20192019-07-1620190167-73221873-3166https://doi.org/10.1016/j.molliq.2018.12.037https://hdl.handle.net/20.500.12451/4600Silica based SBA-15 nanoparticles functionalized with aminopropyl-trimethoxysilane (AP) groups were prepared, and then Cu2+ ions were decorated onto them via AP (Cu+2-SBA-15), and calculated by ICP-OES as 137.9 mg/g nanoparticles. The characterizations of SBA-15 and SBA-15-AP were examined by XRD, FTIR and BET analysis. Prepared Cu+2-SBA-15 nanoparticles were embedded into cryogel column (Cu+2-SBA-15 NEC) photographed by SEM, and tried to investigate some adsorption parameters against to lysozyme selected as model protein. Lysozyme adsorption studies were conducted in a continuous system. The maximum adsorption capacity (1275.2 mg/g particles) of Cu+2-SBA-15 nanoparticles was obtained at phosphate buffer of pH 7.0 with initial lysozyme concentration of 1 mg/mL. According to examined Langmuir and Freundlich adsorption isotherms, Langmuir isotherm was fitted the best to this study. Composite cryogels were also used for 30 times in adsorption-desorption experiments. Purification capability of matrix was expressed as 88.5% with 913 purification yield from hen egg white (HEW) by means of SDS-PAGE. (C) 2018 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/embargoedAccessSBA-15 NanoparticlesEmbedding CryogelsIMACMonolithLysozyme AdsorptionArticle27648048710.1016/j.molliq.2018.12.037Q1WOS:000459528600053N/A