Acet, ÖmürAli Noma, Samir AbbasÖnal Acet, BurcuDikici, EmrahOsman, BilgenOdabaşı, Mehmet2023-02-232023-02-2320230731-7085https:/dx.doi.org/10.1016/j.jpba.2023.115250https://hdl.handle.net/20.500.12451/10273In this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 °C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.eninfo:eu-repo/semantics/embargoedAccessAsparagineL-asparaginase ActivityMolecular ImprintingRemovArticle22610.1016/j.jpba.2023.11525036657352Q1WOS:000971451800001Q2