Dikici, EmrahÖnal Acet, BurcuBozdoğan, BetülAcet, ÖmürHalets-Bui, InessaShcharbin, DzmitryOdabaşı, Mehmet2025-04-172025-04-17202519961944https://dx.doi.org/10.3390/ma18010108https://hdl.handle.net/20.500.12451/13069Peptide-based nanomaterials can be easily functionalized due to their functional groups, as well as being biocompatible, stable under physiological conditions, and nontoxic. Here, diphenylalanineamide-based nanomaterials (FFANMs) were synthesized, decorated with Ca2+ ions to set the surface charge, and characterized for possible use in gene delivery and drug release studies. FFANMs were characterized by SEM, TEM, dynamic light scattering (DLS), and LC-MS/MS. Corona formation and biocompatible studies were also carried out. Some of the data obtained are as follows: FFANMs have a diameter of approximately 87.93 nm. While the zeta potentials of FFANMs and Ca2+@FFANMs were −20.1 mV and +9.3 mV, respectively, after corona formation with HSA and IgG proteins, they were shifted to −7.6 mV and −3.7 mV, respectively. For gene delivery studies, zeta potentials of Ca2+@FFANMs and DNA interactions were also studied and found to shift to −9.7 mV. Cytotoxicity and biocompatibility studies of NMs were also studied on HeLa and HT29 cell lines, and decreases of about 5% and 10% in viability at the end of 24 h and 72 h incubation times were found. We think that the results obtained from this study will assist the groups working in the relevant field. © 2024 by the authors.eninfo:eu-repo/semantics/openAccessDiphenylalanineamide DipeptideNanomaterialsProtein CoronaSelf-assemblyBiocompatibilityArticle18110.3390/ma180101082-s2.0-85214449427Q2