Güngör, Şükrüİnanç, Muhammed EnesAvdatek, FatihTürkmen, RuhiTaşdemir, Umut2021-12-132021-12-132021https:/dx.doi.org/10.9775/kvfd.2021.26023https://hdl.handle.net/20.500.12451/8927This study aimed to determine the probable protective effect of gallic acid (GA) on the spermatological parameters of frozen-thawed bull semen. Ejaculates were collected from four Holstein bulls. The mixed ejaculate was divided into four equal groups as control (0 pg/mL GA), 50, 100 and 200 mu g/mL GA with a Tris-based extender. All extended groups were cooled, equilibrated into a +4 degrees C cold cabinet and loaded into straws. The straws were then frozen and stored In a liquid nitrogen container (-196 degrees C). Subsequently, samples were thawed in a water bath for analyzing motility and kinematic parameters, morphological integrity, DNA damage and biochemical alterations. GA50 (28.76 +/- 0.51%) and GA100 (29.32 +/- 0.31%) had improved progressive motility in comparison to the other groups (P<0.05). The highest total motility (69.71 +/- 0.52%) was detected in GA100 (P<0.05). Besides, the lowest DNA damage and total abnormality values were detected in the GA100 group (P<0.05). GA100 had the highest total antioxidant capacity, as well as the lowest malondialdehyde (MDA) level (P<0.05). In conclusion, GA 50 and 100 mu g/mL protects the progressive motility, morphological and DNA integrity by improving the total antioxidant status from the harmful effects of the freezing and thawing protocol.enAttribution-NonCommercial-NoDerivs 3.0 United Statesinfo:eu-repo/semantics/openAccessAntioxidantBull SemenDNA DamageGallic AcidArticle27563363910.9775/kvfd.2021.26023WOS:000704282200013Q4