Kazak, FilizAkçakavak, GökhanAlakuş, İbrahimAlakuş, HalilKırgız, ÖmerKarataş, ÖzhanDeveci, Mehmet Zeki YılmazCoşkun, Pınar2024-07-242024-07-242024https://hdl.handle.net/20.500.12451/12208Testicular torsion is an urological emergency and can lead to ischemia damage and testicular loss if not diagnosed in time. Proanthocyanidin is reported to have anti-inflammatory and antioxidant properties. The current study aimed to examine the possible effects of proanthocyanidin (P) on the testis in torsion/detorsion (T/D)-induced testicular ischemia/reperfusion (I/R) injury in rats. Forty rats were divided into four groups (n=10 for each): sham-operated (sham), I/R, I/R + P100 (100?mg/kg, 30?min before torsion), and I/R + P200 (200?mg/kg, 30?min before torsion). Testicular T/D was performed on the left testicle by 3?hours of torsion at 720° clockwise, followed by 3?hours of detorsion. In the I/R group, an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH), vitamin C (Vit C), glutathione peroxidase (GPx), glucose-6-phosphate dehydrogenase (G6PD) values were determined compared to the sham group (p<0.001). Moreover, an increase in the expression of cleaved caspase-3 and Bcl2-associated X protein (Bax), a decrease in the expression of B-cell lymphoma 2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) were detected in the I/R group (p<0.001). Histopathologically, it was determined that the Johnsen and Cosentino scores of the testicles were irregular in the I/R group (p<0.001). Proanthocyanidin treatment caused a decrease in MDA, cleaved caspase-3 and Bax levels and an increase in GSH, Vit C, GPx, G6PD, Bcl-2 and PCNA values. Additionally, Johnsen and Cosentino rearranged the scores. The present findings revealed the protective and curative effects of proanthocyanidin in organ damage due to testicular torsion/detorsion-induced ischemia/reperfusion with their antioxidative and antiapoptotic properties.eninfo:eu-repo/semantics/embargoedAccessOxidative StressBaxBcl-2Cleaved Caspase-3ProanthocyanidinTorsion/detorsionArticle89-Q3N/A