Alkan, HüseyinCömert, Şeyda CeylanGürbüz, FatmaDoğru, Mehmet İlkerOdabaşı, Mehmet13.07.20192019-07-2913.07.20192019-07-2920172169-14012169-141Xhttps://doi.org/10.3109/21691401.2015.1129627https://hdl.handle.net/20.500.12451/6243In this study, chromatographic performance of Cu2+-attached pumice particles embedded to monolithic cryogels (Cu2+-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.eninfo:eu-repo/semantics/closedAccessComposite CryogelHuman Serum Albumin ChromatographyMetal ChelateParticle EmbeddingPumiceArticle451909710.3109/21691401.2015.112962726755205Q1WOS:000391459800014N/A