Jabir, Majid S.Abood, Noor A.Jawad, Marwa H.Öztürk, KamileKadhim, HaiderAlbukhaty, SalimAl-Shammari, AhmedAlMalki, Faizah A.Albaqami, JawaherSulaiman, Ghassan M.2023-01-162023-01-1620221066-7857https:/dx.doi.org/10.1080/10667857.2022.2133073https://hdl.handle.net/20.500.12451/9943We investigated the anti-cancer properties of gold nanoparticles loaded TNF- and CALNN peptides, which we proposed as a potential drug delivery system using in vitro and in vivo models. The binding of GNPs-TNF- and GNPs-TNF-CALNN was characterized using a UV, ELISA and SEM analysis. The outcomes demonstrated that a novel drug delivery system had an anti-proliferative activity against breast cancer cell lines through a mechanism of apoptosis induction. In vivo model involved studying the cytotoxic influence of a drug delivery system GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN when applied to the transplanted AN-3 cell line. tumor sections were examined using microarray. In-vivo studies demonstrated that GNPs alone had less of a growth inhibitory effect on tumors implanted in mice when compared to GNPs-TNF–CALNN combined therapy. The cytotoxic assay showed that GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN exhibit selective toxicity towards cancer cells, inducing cell apoptosis through activation of caspase-3 and 7, p53 protein.eninfo:eu-repo/semantics/embargoedAccessApoptosisBreast CancerCALNNGold NanoparticlesTNF- ?Article37143152316610.1080/10667857.2022.2133073Q1WOS:000867615300001Q3