Koç Bilican, BehlülKaraduman Yeşildal, TuğçeTornacı, SelayCansaran Duman, DemetÖner, Ebru ToksoyGül, SerkanKaya, Murat2025-09-182025-09-18202520734360https://doi.org/10.3390/polym17152046https://hdl.handle.net/20.500.12451/14448Extensive research on amphibians has focused on areas such as morphological and molecular taxonomy, ecology, embryology, and molecular phylogeny. However, the structure and biotechnological potential of egg jelly—which plays a protective and nutritive role for embryos—have remained largely unexplored. This study presents, for the first time, a detailed physicochemical analysis of the egg jelly of Pelophylax ridibundus, an amphibian species, using Fourier Transform Infrared Spectroscopy, Thermogravimetric Analyzer, X-ray Diffraction, and elemental analysis. The carbohydrate content was determined via High-Performance Liquid Chromatography analysis, and the protein content was identified using Liquid Chromatography-Tandem Mass Spectrometry analysis. Additionally, it was revealed that this jelly exhibits a significant cytotoxic effect on melanoma cells (viability < 30%) while showing no cytotoxicity on healthy dermal fibroblast cells (viability > 70%). Consequently, this non-toxic, biologically derived, and cultivable material is proposed as a promising candidate for cancer applications, paving the way for further research in the field.eninfo:eu-repo/semantics/closedAccessAmphibian Egg JellyAnticancerBiocompatible MaterialMelanomaPelophylax RidibundusReal-Time Cell AnalysisArticle171510.3390/polym171520462-s2.0-105013200602Q1WOS:001550659600001Q1