Alper, MeltemAydemir, T.Köçkar, F.2021-07-052021-07-0520210026-8933https:/dx.doi.org/10.1134/S0026893321030031https://hdl.handle.net/20.500.12451/8309*Alper, Meltem ( Aksaray, Yazar )Abstract: Collagens are the main components of human tissues. Various regulatory factors and cytokines may influence expression levels for collagen-encoding genes, and, therefore, contrubite to some collagen-associated pathologies. In this study, we demonstrate regulatory effects of USF1 on expression of genes encoding fibrillar collagen types I, II, and III in osteoblastic Saos-2 and MG-63 cells. An ectopic expression of the human USF1 led to a decrease in both mRNA and protein expression levels of the collagen-encoding genes mentioned above. ADAMTS-3 is a proteinase primarily responsible for the amino-terminal cleavage of type I and type II collagen precursors. The ADAMTS-3 promoter region contains potential binding sites for USF1. Here we show that an overexpression of USF1 lead to a decrease in ADAMTS-3 mRNA and protein expression levels. In co-transfection studies, USF1 negatively regulated ADAMTS-3 promoter activity. Further, in EMSA studies, we showed that USF1 binds to the ADAMTS-3 promoter region. In conclusion, it seems that ADAMTS-3 and USF1 contribute to the regulation of collagen encoding genes in osteosarcoma.eninfo:eu-repo/semantics/closedAccessADAMTS-3CollagenOsteosarcomaTranscriptional RegulationUSF1Article----10.1134/S002689332103003134432781Q4WOS:000656110200003Q4