Tekin, NeslihanÖztürk, KamileBaran, TalatKerimoğlu, BarışTarhan, MehtapMenteş, Ayfer13.07.20192019-07-1613.07.20192019-07-1620171060-13251520-5738https://doi.org/10.1080/10601325.2017.1294449https://hdl.handle.net/20.500.12451/4397In the investigation for alternative chemotherapeutic strategies against leukemia, Pd(II) complexes were synthesized and investigated for cytotoxic and apoptotic properties on two human leukemia cell lines (HL-60 and K562). Pd(II) complexes (Pd-5a and Pd-6a) with 5a and 6a as ligands were synthesized and characterized by H-1-NMR and F-TIR. The cytotoxicity of the compounds was quantified using MTT method. Bax, Bcl-2, and caspase 3 gene expression levels were estimated using RT-qPCR. Here we show that Pd(II) complexes have important cytotoxic activity on human leukemia cell lines. RT-qPCR indicated that Bax and caspase 3 gene expression levels were increased after 24h treatment with Pd-5a and Pd-6a complexes in both HL-60 and K562 cells at some selected dose. Furthermore, Bcl-2 gene expression level decreased after 24h treatment with Pd-5a and Pd-6a complexes in K562 cells at all selected dose. In HL-60 cells, only one selected Pd-5a dose (25 mu M) decreased the gene expression level of Bcl-2. The results obtained in the present investigation indicate that these two newly synthesized Pd(II) complexes have apoptotic effects at appropriate doses through caspase 3 and Bax genes and might represent a novel potentially active agents for the management of human leukemia cell lines.eninfo:eu-repo/semantics/closedAccessApoptosisBaxBcl-2Caspase 3LeukemiaPalladium (II) ComplexesArticle54526327010.1080/10601325.2017.1294449Q2WOS:000399809000001N/A