Tan, Gamzeİlk, SedefFoto, Fatma Z.Foto, EgemenSağlam, Necdet2021-05-252021-05-252021https:/dx.doi.org/10.12989/anr.2021.10.2.139https://hdl.handle.net/20.500.12451/7963Tan, Gamze ( Aksaray, Yazar )In this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells -lung (A549), cervix (HeLa) and colon (WiDr) for 24, 48 and 72 h to evaluate their anti -proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti -cancer agents, providing greater efficacy against cancer cells.eninfo:eu-repo/semantics/closedAccessPropolissilver NanoparticlesDPPHSRB AssayAnti -cancer ActivityCytotoxicityArticle10213915010.12989/anr.2021.10.2.139N/AWOS:000643257800004Q1