Karahaliloğlu, ZeynepKılıçay, Ebru2022-05-162022-05-1620221229-91971875-0052https:/dx.doi.org/10.1007/s12221-022-4830-8https://hdl.handle.net/20.500.12451/9389Guided tissue (GTR) and guided bone regeneration (GBR) are well-established surgical procedures in orthopedics and dentistry. Recently, the most prevalent reason of unsuccessful GBR membranes-mediated tissue regeneration is infection. Several antibiotics have been frequently used to overcome this problem. Further, the antibiotic resistance limits their profitability, and therefore, alternative antibacterial GBR membranes are highly in demand. With this focus, the goal of the study was to develop a novel antibacterial GBR membrane composed of a Poly[(R)-3-hydroxybutyric acid] (PHB) fibrillar membrane loaded with lauric acid (LA) for bone regeneration. New type of GBR composite membranes were fabricated by PHB/LA composite fibers with three different PHB to LA ratios (PHB:LA=98:2, 90:10, and 80:20, wt%) via electrospinning. Atomic force microscopy (AFM) results of the membranes showed that surface roughness values increased twofold in 20 wt% LA added membrane compared with the pure PHB. On the other hand, colony counting test results implied that 100PHB/LA caused a significant reduction in the amount of viable S. aureus and E. coli, (7.3 and 8.6 log CFU/ml) as compared to the pure PHB (10.1 and 10.9 log CFU/ml), respectively (p<0.05). Hence, it can be concluded that electrospun PHB/LA composite membranes provide great antibacterial effects against two bacteria species while closely mimicking physiological bone environment through their surface properties.Guided tissue (GTR) and guided bone regeneration (GBR) are well-established surgical procedures in orthopedics and dentistry. Recently, the most prevalent reason of unsuccessful GBR membranes-mediated tissue regeneration is infection. Several antibiotics have been frequently used to overcome this problem. Further, the antibiotic resistance limits their profitability, and therefore, alternative antibacterial GBR membranes are highly in demand. With this focus, the goal of the study was to develop a novel antibacterial GBR membrane composed of a Poly[(R)-3-hydroxybutyric acid] (PHB) fibrillar membrane loaded with lauric acid (LA) for bone regeneration. New type of GBR composite membranes were fabricated by PHB/LA composite fibers with three different PHB to LA ratios (PHB:LA=98:2, 90:10, and 80:20, wt%) via electrospinning. Atomic force microscopy (AFM) results of the membranes showed that surface roughness values increased twofold in 20 wt% LA added membrane compared with the pure PHB. On the other hand, colony counting test results implied that 100PHB/LA caused a significant reduction in the amount of viable S. aureus and E. coli, (7.3 and 8.6 log CFU/ml) as compared to the pure PHB (10.1 and 10.9 log CFU/ml), respectively (p<0.05). Hence, it can be concluded that electrospun PHB/LA composite membranes provide great antibacterial effects against two bacteria species while closely mimicking physiological bone environment through their surface properties.eninfo:eu-repo/semantics/embargoedAccessAntibacterial MembraneAntibacterial MembraneElectrospinningElectrospinningGuided Bone RegenerationGuided Bone RegenerationLauric AcidLauric AcidPoly[(R)-3-hydroxybutyric acid] (PHB)Poly[(R)-3-hydroxybutyric acid] (PHB)Article----10.1007/s12221-022-4830-8Q2WOS:000780754500039Q2