İnanan, Burak Evren2019-11-042019-11-042020https://dx.doi.org/10.1111/are.14368https://hdl.handle.net/20.500.12451/6943The aim of this study was to evaluate the effects of Roswell Park Memorial Institute(RPMI) 1,640 medium on chilled storage of eggs and spermatozoa of rainbow trout (Oncorhynchus mykiss). After 3 days of storage, eggs in RPMI 1,640 media (pH 8.2, 9 and 10), Cortland medium and coelomic fluid were inseminated with fresh sperma?tozoa (Experiment 1). Eggs in RPMI 1,640 medium at pH 8.2 shown the lowest thio?barbituric acid?reactive substances (TBARS, 0.053 ± 0.003 nmol/ml) and pH changes (from 8.20 ± 0.01 to 8.18 ± 0.01), the highest fertilization rate (82 ± 3%). Undiluted and diluted spermatozoa at ratios of 1:2 and 1:9 with RPMI 1,640 media (pH 8.2, 9 and 10) and Cortland medium were inseminated with fresh eggs (Experiment 2). Spermatozoa in RPMI 1,640 medium at pH 9 (1:9) caused the lowest TBARS content (0.037 ± 0.002 nmol/ml) and pH changes (from 9.00 ± 0.01 to 8.98 ± 0.01), the high?est fertilization rate (77 ± 2%) and motility parameters. Based on Experiments 1 and 2, eggs and spermatozoa were stored for another 3 days in RPMI 1,640 medium at pH 8.2 and 9 (1:9) respectively (Experiment 3). Fertilization rate of storage eggs and spermatozoa in Experiment 3 was 79 ± 5%, showing successfully storage of rainbowtrout gametes with the same medium....eninfo:eu-repo/semantics/embargoedAccessChilled StorageEggsFertilizationOncorhynchus MykissSpermatozoaArticle----10.1111/are.14368Q2N/A