Hadroug, AldjiaZengin, GökhanBelhattab, RachidÇakmak, Yavuz Selim2024-11-192024-11-192024https:/dx.doi.org/10.1080/14786419.2024.2419498https://hdl.handle.net/20.500.12451/12653This research explored the impact of methanolic extracts from the fruits and leaves of Ilex aquifolium L., a rare Algerian species, on several enzymes, focusing on tyrosinase, ?-amylase, ?-glucosidase, and cholinesterases, as well as their antioxidant potential in vitro assays, including phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and MCA. HPLC-DAD analysis identified chlorogenic acid, rosmarinic acid, hesperidin, and quercetin as predominant phenolic components. Both extracts exhibited notable antioxidant properties, up to 250.08?mg TE/g for fruits and 237.08?mg TE/g for leaf extracts. Enzyme inhibitory assays demonstrated significant inhibition against AChE and BChE with maximum activities of 2.34?mg GALAE/g in leaves and 4.32?mg GALAE/g in fruits. Additionally, considerable tyrosinase inhibition was observed in leaves at 39.69?mg KAE/g. These findings highlight I. aquifolium L. as a valuable source for investigating natural antioxidants and enzyme inhibitors, particularly for possible cosmetics and food additives applications.eninfo:eu-repo/semantics/embargoedAccessIlex Aquifolium LBioactive CompoundsAntioxidant ActivityEnzyme Inhibition EffectArticle10.1080/14786419.2024.2419498Q1